Q5HED0 (ACPS_STAAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holo-[acyl-carrier-protein] synthase
Short name=Holo-ACP synthase
EC=2.7.8.7

Alternative name(s):
4'-phosphopantetheinyl transferase AcpS

Gene names

Name:	acpS
Ordered Locus Names:	SACOL2061

Organism

Staphylococcus aureus (strain COL) [Complete proteome] [HAMAP]

Taxonomic identifier

93062 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein By similarity. HAMAP MF_00101
Catalytic activity	CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]. HAMAP MF_00101
Cofactor	Magnesium By similarity. HAMAP MF_00101
Subcellular location	Cytoplasm By similarity HAMAP MF_00101.
Sequence similarities	Belongs to the P-Pant transferase superfamily. AcpS family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW macromolecule biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	holo-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 119

119

Holo-[acyl-carrier-protein] synthase HAMAP MF_00101

PRO_0000175700

Sites

Metal binding

8

1

Magnesium By similarity

Metal binding

59

1

Magnesium By similarity

Secondary structure

1

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119

Helix Strand Turn

Details...

Beta strand

8 – 11

4

Helix

12 – 20

9

Helix

23 – 25

3

Helix

26 – 29

4

Helix

32 – 39

8

Helix

44 – 51

8

Helix

55 – 64

10

Helix

75 – 77

3

Beta strand

80 – 82

3

Beta strand

88 – 90

3

Beta strand

96 – 103

8

Beta strand

105 – 115

11

Sequences

	Sequence		Length	Mass (Da)
	Q5HED0 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 0B828A811774B138	FASTA	119	13,606
	10 20 30 40 50 60 MIHGIGVDLI EIDRIQALYS KQPKLVERIL TKNEQHKFNN FTHEQRKIEF LAGRFATKEA 70 80 90 100 110 FSKALGTGLG KHVAFNDIDC YNDELGKPKI DYEGFIVHVS ISHTEHYAMS QVVLEKSAF

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: COL.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CP000046 Genomic DNA. Translation: AAW37023.1.

RefSeq

YP_186877.1. NC_002951.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3F09	X-ray	1.82	A/B/C	1-119	[»]

ProteinModelPortal

Q5HED0.

ModBase

Search...

Protein-protein interaction databases

STRING

Q5HED0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBSTAT00000009170; EBSTAP00000008905; EBSTAG00000009169.

GeneID

3238092.

GenomeReviews

Gene locus SACOL2061 in contig CP000046_GR.

KEGG

sac:SACOL2061.

PATRIC

19530397. VBIStaAur112458_2010.

TIGR

SACOL2061.

Phylogenomic databases

eggNOG

COG0736.

GeneTree

EBGT00050000025046.

HOGENOM

HBG734479.

OMA

VHVSITH.

ProtClustDB

PRK00070.

Enzyme and pathway databases

BioCyc

SAUR93062:SACOL2061-MONOMER.

Family and domain databases

HAMAP

MF_00101. AcpS.
[Tree]

InterPro

IPR008278. 4-PPantetheinyl_Trfase.
IPR002582. ACPS.
IPR004568. PPantethiene-prot_Trfase.
[Graphical view]

Gene3D

G3DSA:3.90.470.20. G3DSA:3.90.470.20. 1 hit.

KO

K00997.

Pfam

PF01648. ACPS. 1 hit.
[Graphical view]

ProDom

PD004282. PPantethiene-prot_Trfase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF56214. 4-PPT_transf. 1 hit.

TIGRFAMs

TIGR00516. AcpS. 1 hit.
TIGR00556. Pantethn_trn. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ACPS_STAAC

Accession

Primary (citable) accession number: Q5HED0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program