Q5HE66 (LUXS_STAAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-ribosylhomocysteine lyase
EC=4.4.1.21

Alternative name(s):
AI-2 synthesis protein
Autoinducer-2 production protein LuxS

Gene names

Name:	luxS
Ordered Locus Names:	SACOL2126

Organism

Staphylococcus aureus (strain COL) [Complete proteome] [HAMAP]

Taxonomic identifier

93062 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity. HAMAP MF_00091
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091
Cofactor	Binds 1 iron ion per subunit By similarity. HAMAP MF_00091
Subunit structure	Homodimer By similarity. HAMAP MF_00091
Sequence similarities	Belongs to the LuxS family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Quorum sensing
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	quorum sensing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	S-ribosylhomocysteine lyase HAMAP MF_00091		PRO_0000172253
Sites
	Metal binding	56	1	Iron By similarity
	Metal binding	60	1	Iron By similarity
	Metal binding	123	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HE66 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: CC19EAE44C14C248	FASTA	156	17,514
	10 20 30 40 50 60 MTKMNVESFN LDHTKVVAPF IRLAGTMEGL NGDVIHKYDI RFKQPNKEHM DMPGLHSLEH 70 80 90 100 110 120 LMAENIRNHS DKVVDLSPMG CQTGFYVSFI NHDNYDDVLN IVEATLNDVL NATEVPACNE 130 140 150 VQCGWAASHS LEGAKTIAQA FLDKRNEWHD VFGTGK

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: COL.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000046 Genomic DNA. Translation: AAW38436.1.
RefSeq	YP_186941.1. NC_002951.2.
3D structure databases
ProteinModelPortal	Q5HE66.
SMR	Q5HE66. Positions 3-151.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HE66.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000009354; EBSTAP00000009089; EBSTAG00000009353.
GeneID	3237462.
GenomeReviews	Gene locus SACOL2126 in contig CP000046_GR.
KEGG	sac:SACOL2126.
PATRIC	19530519. VBIStaAur112458_2071.
TIGR	SACOL2126.
Phylogenomic databases
eggNOG	COG1854.
GeneTree	EBGT00050000025271.
HOGENOM	HBG347473.
OMA	PHGDTIT.
ProtClustDB	PRK02260.
Enzyme and pathway databases
BioCyc	SAUR93062:SACOL2126-MONOMER.
Family and domain databases
HAMAP	MF_00091. LuxS. [Tree]
InterPro	IPR011249. Metalloenz_metal-bd. IPR003815. S-ribosylhomocysteinase. [Graphical view]
Gene3D	G3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
KO	K07173.
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. S-ribosylhomocysteinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF63411. Metalloenz_metal-bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LUXS_STAAC

Accession

Primary (citable) accession number: Q5HE66

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program