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Protein

Mannitol-specific phosphotransferase enzyme IIA component

Gene

mtlF

Organism
Staphylococcus aureus (strain COL)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport (By similarity).By similarity

Catalytic activityi

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei63 – 631Tele-phosphohistidine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciSAUR93062:GCEP-2141-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannitol-specific phosphotransferase enzyme IIA component (EC:2.7.1.-)
Alternative name(s):
EIIA-Mtl
EIII-Mtl
PTS system mannitol-specific EIIA component
Gene namesi
Name:mtlF
Synonyms:mtlA
Ordered Locus Names:SACOL2148
OrganismiStaphylococcus aureus (strain COL)
Taxonomic identifieri93062 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000530 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 144143Mannitol-specific phosphotransferase enzyme IIA componentPRO_0000186636Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotrimer. Seems to be a monomer when not phosphorylated (By similarity).By similarity

Protein-protein interaction databases

STRINGi93062.SACOL2148.

Structurei

3D structure databases

ProteinModelPortaliQ5HE46.
SMRiQ5HE46. Positions 8-139.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 142140PTS EIIA type-2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similaritiesi

Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DTZ. Bacteria.
COG4668. LUCA.
HOGENOMiHOG000227563.
KOiK02798.
OMAiISIVQVP.
OrthoDBiEOG6XDGX2.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5HE46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELFSNDNI FLNVNVNSQN EAIEKAGKAL VDSGAVTDAY IQAMKDREQV
60 70 80 90 100
VSTFMGNGLA IPHGTDEAKT NVIHSGLTLL QIPEGVDWDG EVVKVVVGIA
110 120 130 140
GKDGEHLDLL SKIAITFSEE ENVDRIVQAK SAEEIKQVFE EADA
Length:144
Mass (Da):15,542
Last modified:January 23, 2007 - v3
Checksum:iB166851EEBC3A5AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09927 Genomic DNA. Translation: CAB55331.1.
CP000046 Genomic DNA. Translation: AAW38456.1.
RefSeqiWP_001292149.1. NC_002951.2.

Genome annotation databases

EnsemblBacteriaiAAW38456; AAW38456; SACOL2148.
KEGGisac:SACOL2148.
PATRICi19530561. VBIStaAur112458_2092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09927 Genomic DNA. Translation: CAB55331.1.
CP000046 Genomic DNA. Translation: AAW38456.1.
RefSeqiWP_001292149.1. NC_002951.2.

3D structure databases

ProteinModelPortaliQ5HE46.
SMRiQ5HE46. Positions 8-139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93062.SACOL2148.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW38456; AAW38456; SACOL2148.
KEGGisac:SACOL2148.
PATRICi19530561. VBIStaAur112458_2092.

Phylogenomic databases

eggNOGiENOG4105DTZ. Bacteria.
COG4668. LUCA.
HOGENOMiHOG000227563.
KOiK02798.
OMAiISIVQVP.
OrthoDBiEOG6XDGX2.

Enzyme and pathway databases

BioCyciSAUR93062:GCEP-2141-MONOMER.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Transcriptional analysis of arginase gene cluster in Staphylococcus aureus."
    Wu S.W., de Lencastre H., Tomasz A.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
    Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H.
    , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
    J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: COL.

Entry informationi

Entry nameiPTMA_STAAC
AccessioniPrimary (citable) accession number: Q5HE46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: January 23, 2007
Last modified: March 16, 2016
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.