Reviewed, UniProtKB/Swiss-Prot Q5HD29 (LDHD_STAAC)

Names and origin

Protein names

Recommended name:
    D-lactate dehydrogenase
      Short name=D-LDH
    EC=1.1.1.28
Alternative name(s):
    D-specific D-2-hydroxyacid dehydrogenase

Gene names

Name:	ldhD
Synonyms:	ddh
Ordered Locus Names:	SACOL2535

Organism

Staphylococcus aureus (strain COL) [Complete proteome] [HAMAP]

Taxonomic identifier

93062 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-lactate + NAD(+) = pyruvate + NADH.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	D-lactate dehydrogenase		PRO_0000075959
Sites
	Active site	235	1	By similarity
	Active site	264	1	By similarity
	Active site	296	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HD29-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: A483404D1D240230	FASTA	330	36,682
	10 20 30 40 50 60 MTKIMFFGTR DYEKEMALNW GKKNNVEVTT SKELLSSATV DQLKDYDGVT TMQFGKLEND 70 80 90 100 110 120 VYPKLESYGI KQIAQRTAGF DMYDLDLAKK HNIVISNVPS YSPETIAEYS VSIALQLVRR 130 140 150 160 170 180 FPDIERRVQA HDFTWQAEIM SKPVKNMTVA IIGTGRIGAA TAKIYAGFGA TITAYDAYPN 190 200 210 220 230 240 KDLDFLTYKD SVKEAIKDAD IISLHVPANK ESYHLFDKAM FDHVKKGAIL VNAARGAVIN 250 260 270 280 290 300 TPDLIAAVND GTLLGAAIDT YENEAAYFTN DWTNKDIDDK TLLELIEHER ILVTPHIAFF 310 320 330 SDEAVQNLVE GGLNAALSVI NTGTCETRLN

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000046 Genomic DNA. Translation: AAW37312.1.
RefSeq	YP_187328.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3238242.
GenomeReviews	Gene locus SACOL2535 in contig CP000046_GR.
KEGG	sac:SACOL2535.
TIGR	SACOL2535.
Phylogenomic databases
HOGENOM	Q5HD29.
Enzyme and pathway databases
BioCyc	SAUR93062:SACOL2535-MON.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DHase. IPR006140. D-isomer_2_OHA_DHase_NAD-bd. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHD_STAAC

Accession

Primary (citable) accession number: Q5HD29

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	February 15, 2005
Last modified:	November 25, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)