Q5HC85 (PTH_EHRRW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidyl-tRNA hydrolase
Short name=PTH
EC=3.1.1.29

Gene names

Name:	pth
Ordered Locus Names:	Erum0910, ERWE_CDS_00880

Organism

Ehrlichia ruminantium (strain Welgevonden) [Complete proteome] [HAMAP]

Taxonomic identifier

254945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Ehrlichia

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis By similarity. HAMAP MF_00083
Catalytic activity	N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA. HAMAP MF_00083
Subunit structure	Monomer By similarity. HAMAP MF_00083
Subcellular location	Cytoplasm By similarity HAMAP MF_00083.
Sequence similarities	Belongs to the PTH family.
Sequence caution	The sequence CAI26582.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminoacyl-tRNA hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Peptidyl-tRNA hydrolase HAMAP MF_00083		PRO_0000187737

Sequences

	Sequence		Length	Mass (Da)
	Q5HC85 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: E789169FDB77A7B2	FASTA	193	21,839
	10 20 30 40 50 60 MLHLLVGLGN PGKEYELTRH NVGFMIIDAI MHHFLFPDFK KKHNALISSG SIRSHKVILA 70 80 90 100 110 120 KPYTFMNNSG TPISSIVKLY KIPLDNIIVF HDETDIDFCT IRIKKGGGNA GHNGLKSIDT 130 140 150 160 170 180 LLGRDYWRIR FGIGHPSNGY DLSYHVLSQF NNLNAVNNTI SNIIEHISLL FENDKSIFKN 190 KVKDLIKYTD ISS

References

[1]

"The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number." Collins N.E., Liebenberg J., de Villiers E.P., Brayton K.A., Louw E., Pretorius A., Faber F.E., van Heerden H., Josemans A., van Kleef M., Steyn H.C., van Strijp M.F., Zweygarth E., Jongejan F., Maillard J.C., Berthier D., Botha M., Joubert F. , Corton C.H., Thomson N.R., Allsopp M.T., Allsopp B.A. Proc. Natl. Acad. Sci. U.S.A. 102:838-843(2005) [PubMed: 15637156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Welgevonden.

[2]

"Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity." Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D. J. Bacteriol. 188:2533-2542(2006) [PubMed: 16547041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Welgevonden.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR767821 Genomic DNA. Translation: CAH57806.1. CR925678 Genomic DNA. Translation: CAI26582.1. Different initiation.
RefSeq	YP_179958.1. NC_005295.2. YP_196964.1. NC_006832.1.
3D structure databases
ProteinModelPortal	Q5HC85.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HC85.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3232627. 3261433.
GenomeReviews	Gene locus Erum0910 in contig CR767821_GR. Gene locus ERWE_CDS_00880 in contig CR925678_GR.
KEGG	eru:Erum0910. erw:ERWE_CDS_00880.
PATRIC	20579913. VBIEhrRum92411_0092.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0193.
HOGENOM	HBG610927.
PhylomeDB	Q5HC85.
ProtClustDB	PRK05426.
Enzyme and pathway databases
BioCyc	ERUM254945-1:ERUM0910-MONOMER. ERUM254945:ERWE_CDS_00880-MONOMER.
Family and domain databases
HAMAP	MF_00083. Pept_tRNA_hydro_bact. [Tree]
InterPro	IPR001328. Pept_tRNA_hydro. IPR018171. Pept_tRNA_hydro_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.1470. Pept_tRNA_hydro. 1 hit.
KO	K01056.
PANTHER	PTHR17224. Pept_tRNA_hydro. 1 hit.
Pfam	PF01195. Pept_tRNA_hydro. 1 hit. [Graphical view]
SUPFAM	SSF53178. Pept_tRNA_hydro. 1 hit.
TIGRFAMs	TIGR00447. Pth. 1 hit.
PROSITE	PS01195. PEPT_TRNA_HYDROL_1. 1 hit. PS01196. PEPT_TRNA_HYDROL_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTH_EHRRW

Accession

Primary (citable) accession number: Q5HC85
Secondary accession number(s): Q5FCM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program