Q5HC73 (Q5HC73_EHRRW) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108
EC=2.7.7.60 HAMAP MF_00108

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase HAMAP MF_00108
MEP cytidylyltransferase HAMAP MF_00108

Gene names

Name:	probable ispD EMBL CAH57818.1
Synonyms:	ispD HAMAP MF_00108 EMBL CAI26594.1
Ordered Locus Names:	Erum1030, ERWE_CDS_01000

Organism

Ehrlichia ruminantium (strain Welgevonden) [Complete proteome] [HAMAP]

Taxonomic identifier

254945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Ehrlichia

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108
Molecular function	Nucleotidyltransferase SAAS SAAS001228 HAMAP MF_00108 EMBL CAH57818.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	16	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	24	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	157	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	215	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	Q5HC73 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: A8DB802E3E9E2E1A	FASTA	242	27,895
	10 20 30 40 50 60 MYQFVLLIVA AGNSKRLKGS EKPKQYMELG HYPILYHTIN NVITTPYIGY VKVVIRQEHE 70 80 90 100 110 120 YLYNSCIKKI HSSKLLPFSY GGTRRQDSVR IGLESIKYLN PDFVIIHDAC RPFISTTLLD 130 140 150 160 170 180 KMLPQLTNYV GVIPVLSITE TIHMINENHT IIKNINRNTL KLVQTPQIYR YTDILSSHML 190 200 210 220 230 240 SYTTDPTKEF PDESSLMIHY NFPIATIEGS NYNLKITTND DLHIATLLYK SMNTKVPNHT LN

References

[1]

"The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number." Collins N.E., Liebenberg J., de Villiers E.P., Brayton K.A., Louw E., Pretorius A., Faber F.E., van Heerden H., Josemans A., van Kleef M., Steyn H.C., van Strijp M.F., Zweygarth E., Jongejan F., Maillard J.C., Berthier D., Botha M., Joubert F. , Corton C.H., Thomson N.R., Allsopp M.T., Allsopp B.A. Proc. Natl. Acad. Sci. U.S.A. 102:838-843(2005) [PubMed: 15637156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Welgevonden [ARC-OVI].

[2]

"Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity." Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D. J. Bacteriol. 188:2533-2542(2006) [PubMed: 16547041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Welgevonden [CIRAD].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR767821 Genomic DNA. Translation: CAH57818.1. CR925678 Genomic DNA. Translation: CAI26594.1.
RefSeq	YP_179970.1. NC_005295.2. YP_196976.1. NC_006832.1.
3D structure databases
ProteinModelPortal	Q5HC73.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HC73.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3233518. 3260913.
GenomeReviews	Gene locus Erum1030 in contig CR767821_GR. Gene locus ERWE_CDS_01000 in contig CR925678_GR.
KEGG	eru:Erum1030. erw:ERWE_CDS_01000.
PATRIC	20579947. VBIEhrRum92411_0108.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	REQQDFW.
PhylomeDB	Q5HC73.
ProtClustDB	CLSK749414.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q5HC73_EHRRW

Accession

Primary (citable) accession number: Q5HC73
Secondary accession number(s): Q5FCQ0

Entry history

Integrated into UniProtKB/TrEMBL:	February 15, 2005
Last sequence update:	February 15, 2005
Last modified:	December 14, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)