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Q5HBM8 (SYL_EHRRW) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Erum3010, ERWE_CDS_03070
OrganismEhrlichia ruminantium (strain Welgevonden) [Complete proteome] [HAMAP]
Taxonomic identifier254945 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia

Protein attributes

Sequence length830 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 830830Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334755

Regions

Motif34 – 4411"HIGH" region HAMAP-Rule MF_00049
Motif592 – 5965"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5951ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5HBM8 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 255DC2566DEC9A19

FASTA83095,353
        10         20         30         40         50         60 
MHYNFKEVER DVQEKWDFTV NVKDVQCYVL EMFPYPSGNI HMGHLRNYTI GDVIARYKRA 

        70         80         90        100        110        120 
CGLHVFHPIG WDAFGLPAEN AALSYNISPQ VWTRKNIDNM RSQLKSIGLS YNWDKEFATC 

       130        140        150        160        170        180 
DAEYYKYEQE FFLDFLKYGL AYRKESLVNW DPVDQTVLAN EQVIDGRGWR SGAIIEKRKL 

       190        200        210        220        230        240 
SQWFLKITDF AAELLDDLKS LNQWPEKVKL MQERWIGKSE GVIIDFQIVG INEVLQVFTT 

       250        260        270        280        290        300 
SPHTLFGASF IAVSFDHPIL KYVNDVQLIQ LIDNFDRKDL IDESSINTVE KFGINSGLVA 

       310        320        330        340        350        360 
RHPLLDVDLP IYVANFVLMN YGTGAVFCCP AHDQRDFDFA KKYSLPIRQV IFPEQNVDLE 

       370        380        390        400        410        420 
QEAYVGSGIM GCSGFLDGMT VDDAKKSMIE KLISLGICKK QVYYRLHDWG ISRQRYWGCP 

       430        440        450        460        470        480 
IPIIYCKKCG IVPVDKKDLP ITLPEDIDFT KSGNPLDNHP TWKYTKCPSC GADAKRETDT 

       490        500        510        520        530        540 
FDTFFESSWY FAAFCGIGKG IDKDVCNKLL PVDYYIGGIE HAVLHLLYSR FFCRALTRCG 

       550        560        570        580        590        600 
YFDVKEPFSS LITQGMVCHS TYLDKYGNYL FPEDGKKMIQ EGKYVTVGRA EKMSKSKKNV 

       610        620        630        640        650        660 
VHLDDIIDKY GADSARLFIL SDTPPERDIE WLDENIEGVS RYLNKLWKMI VDYDQLEQNF 

       670        680        690        700        710        720 
VCDNISSDTL EYRINVHKIL NDITNDLEFY RFNCAVAKFR ELSNVISEMI RLSINHHVVS 

       730        740        750        760        770        780 
EAIYILIRVV EPFIPHIAEK LWQIVGGQGM LCNQLWPKVD PQLLIKKNVN IVVQVNGKFI 

       790        800        810        820        830 
KAVSVPNDID DDTLKSIALE VAQRRIGNSS VKNIYIIPGR VINIVITKSS 

« Hide

References

[1]"The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number."
Collins N.E., Liebenberg J., de Villiers E.P., Brayton K.A., Louw E., Pretorius A., Faber F.E., van Heerden H., Josemans A., van Kleef M., Steyn H.C., van Strijp M.F., Zweygarth E., Jongejan F., Maillard J.C., Berthier D., Botha M., Joubert F. expand/collapse author list , Corton C.H., Thomson N.R., Allsopp M.T., Allsopp B.A.
Proc. Natl. Acad. Sci. U.S.A. 102:838-843(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Welgevonden.
[2]"Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity."
Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.
J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Welgevonden.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR767821 Genomic DNA. Translation: CAH58018.1.
CR925678 Genomic DNA. Translation: CAI26801.1.
RefSeqYP_180166.1. NC_005295.2.
YP_197183.1. NC_006832.1.

3D structure databases

ProteinModelPortalQ5HBM8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING254945.Erum3010.

Proteomic databases

PRIDEQ5HBM8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAH58018; CAH58018; Erum3010.
CAI26801; CAI26801; ERWE_CDS_03070.
GeneID3233378.
3260922.
KEGGeru:Erum3010.
erw:ERWE_CDS_03070.
PATRIC20580406. VBIEhrRum92411_0325.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycERUM254945:GJ2L-324-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_EHRRW
AccessionPrimary (citable) accession number: Q5HBM8
Secondary accession number(s): Q5FE96
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 15, 2005
Last modified: April 16, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries