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Q5HB43

- SYI_EHRRW

UniProt

Q5HB43 - SYI_EHRRW

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Ehrlichia ruminantium (strain Welgevonden)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 2 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei650 – 6501ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciERUM254945:GJ2L-536-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Erum4870, ERWE_CDS_05090
    OrganismiEhrlichia ruminantium (strain Welgevonden)
    Taxonomic identifieri254945 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
    ProteomesiUP000001021: Chromosome, UP000006739: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11171117Isoleucine--tRNA ligasePRO_0000098545Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi254945.Erum4870.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5HB43.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi64 – 7411"HIGH" regionAdd
    BLAST
    Motifi647 – 6515"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5HB43-1 [UniParc]FASTAAdd to Basket

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    MCIVKTLHLI THVMKEYNSQ FTEGSDFSLI EEQILEFWKE NNIFKKSIEN     50
    RDEKRRFIFY DGPPFANGLP HYGHLLTGFI KDTVARYKTM AGFRVDRRFG 100
    WDCHGLPAEM LAEKELGVSG KLAIEKFGIE KFNNYCRNSV MKFSREWKQY 150
    IDRQSRWVDF ENDYKTMNLS FMESIMWSFY QLWQKGLIYE SIKIVPYSWA 200
    CQTPLSNFET RIDNAYRQKT SKTVTLAFEL LDAPKSLIVD NITAYKILVW 250
    TTTPWTLPCN LALAISPNIK YCGAIINKEM YIFSRAYLKN FEDHCRKNNI 300
    EYLIHNGDIC YLSLEYLSYK PVFNYFIDIK NAFKVLVADF VVEDEGTGIV 350
    HMAPGFGEDD FILCKKQGIP DIDDKDTSKL LATICPIDDG GKFTERISDF 400
    VNMHVFDTND QIISILKAKN LCFKTDQYLH NYPHCWRTDT PLIYRAMSSW 450
    YVEVTKIKNR MIDLNKDVNW IPSHIRSGQF GKWLENAKDW AISRNRFWGT 500
    PLPVWKSDNP NYPRVDVYGS IKKVFDDIKA LEEDFDIPID DLHRPYIDNL 550
    VRPNPDDPTG KSMMRRVTDV FDCWFESGSM PYAQLHYPFE NKELFENYFP 600
    ADFITEYVAQ TRGWFYTLFV LSTALFDKPP FKNCICHGVV LDTQGQKLSK 650
    RLNNYADPME IFKQYGSDAM RFLMLSHTVS YGGDLLLDKD GVMVRDVIRN 700
    VIKPMWNSYN FFTIYADIDK VNARVISDLD EVDNIMDKYI MCECISTIQS 750
    IFNAMEEFDQ SVGNYGYNIK AACNSIVQFF EVLNNWYIRR CRSRFWSSEI 800
    TKDKVNAYNT LYTVMYYMVK VSAPFLPAIT EAIWQKLNFQ EEESVHLSLL 850
    PNIEHITLKD EDQKNIQYMK LIINICGCVL SIRNVRNIRV RQPLNKITIY 900
    SYNNNDLFNL PVKYQNILLD EINVKSIVFK SNIEDIASFQ LKLNFPELGK 950
    RIPEKMKNLI SLLKSNQWKI LENGQLMLGI REGEHYILED NEYTLNLKVH 1000
    SEFASTITLG PNLLGVLVLD NTLTDELIME GIARDIVRII QQSRKDNKFN 1050
    VSDKIDVVIC TQDKMVKNSV QAWYEYIVQQ TLSLSLVIYE NLDANNVAEY 1100
    CKTTMKDRNL TLFIKKL 1117
    Length:1,117
    Mass (Da):130,645
    Last modified:December 20, 2005 - v2
    Checksum:iC48E96F5C239C230
    GO

    Sequence cautioni

    The sequence CAH58215.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAI27003.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR767821 Genomic DNA. Translation: CAH58215.1. Different initiation.
    CR925678 Genomic DNA. Translation: CAI27003.1. Different initiation.
    RefSeqiYP_180351.1. NC_005295.2.
    YP_197385.2. NC_006832.1.

    Genome annotation databases

    EnsemblBacteriaiCAH58215; CAH58215; Erum4870.
    CAI27003; CAI27003; ERWE_CDS_05090.
    GeneIDi3233369.
    3260905.
    KEGGieru:Erum4870.
    erw:ERWE_CDS_05090.
    PATRICi20580862. VBIEhrRum92411_0543.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR767821 Genomic DNA. Translation: CAH58215.1 . Different initiation.
    CR925678 Genomic DNA. Translation: CAI27003.1 . Different initiation.
    RefSeqi YP_180351.1. NC_005295.2.
    YP_197385.2. NC_006832.1.

    3D structure databases

    ProteinModelPortali Q5HB43.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 254945.Erum4870.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAH58215 ; CAH58215 ; Erum4870 .
    CAI27003 ; CAI27003 ; ERWE_CDS_05090 .
    GeneIDi 3233369.
    3260905.
    KEGGi eru:Erum4870.
    erw:ERWE_CDS_05090.
    PATRICi 20580862. VBIEhrRum92411_0543.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci ERUM254945:GJ2L-536-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Welgevonden.
    2. "Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity."
      Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.
      J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Welgevonden.

    Entry informationi

    Entry nameiSYI_EHRRW
    AccessioniPrimary (citable) accession number: Q5HB43
    Secondary accession number(s): Q5FEI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3