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Q5HB43

- SYI_EHRRW

UniProt

Q5HB43 - SYI_EHRRW

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Erum4870, ERWE_CDS_05090
Organism
Ehrlichia ruminantium (strain Welgevonden)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei650 – 6501ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciERUM254945:GJ2L-536-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Erum4870, ERWE_CDS_05090
OrganismiEhrlichia ruminantium (strain Welgevonden)
Taxonomic identifieri254945 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
ProteomesiUP000001021: Chromosome, UP000006739: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11171117Isoleucine--tRNA ligaseUniRule annotationPRO_0000098545Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi254945.Erum4870.

Structurei

3D structure databases

ProteinModelPortaliQ5HB43.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" regionUniRule annotationAdd
BLAST
Motifi647 – 6515"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HB43-1 [UniParc]FASTAAdd to Basket

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MCIVKTLHLI THVMKEYNSQ FTEGSDFSLI EEQILEFWKE NNIFKKSIEN     50
RDEKRRFIFY DGPPFANGLP HYGHLLTGFI KDTVARYKTM AGFRVDRRFG 100
WDCHGLPAEM LAEKELGVSG KLAIEKFGIE KFNNYCRNSV MKFSREWKQY 150
IDRQSRWVDF ENDYKTMNLS FMESIMWSFY QLWQKGLIYE SIKIVPYSWA 200
CQTPLSNFET RIDNAYRQKT SKTVTLAFEL LDAPKSLIVD NITAYKILVW 250
TTTPWTLPCN LALAISPNIK YCGAIINKEM YIFSRAYLKN FEDHCRKNNI 300
EYLIHNGDIC YLSLEYLSYK PVFNYFIDIK NAFKVLVADF VVEDEGTGIV 350
HMAPGFGEDD FILCKKQGIP DIDDKDTSKL LATICPIDDG GKFTERISDF 400
VNMHVFDTND QIISILKAKN LCFKTDQYLH NYPHCWRTDT PLIYRAMSSW 450
YVEVTKIKNR MIDLNKDVNW IPSHIRSGQF GKWLENAKDW AISRNRFWGT 500
PLPVWKSDNP NYPRVDVYGS IKKVFDDIKA LEEDFDIPID DLHRPYIDNL 550
VRPNPDDPTG KSMMRRVTDV FDCWFESGSM PYAQLHYPFE NKELFENYFP 600
ADFITEYVAQ TRGWFYTLFV LSTALFDKPP FKNCICHGVV LDTQGQKLSK 650
RLNNYADPME IFKQYGSDAM RFLMLSHTVS YGGDLLLDKD GVMVRDVIRN 700
VIKPMWNSYN FFTIYADIDK VNARVISDLD EVDNIMDKYI MCECISTIQS 750
IFNAMEEFDQ SVGNYGYNIK AACNSIVQFF EVLNNWYIRR CRSRFWSSEI 800
TKDKVNAYNT LYTVMYYMVK VSAPFLPAIT EAIWQKLNFQ EEESVHLSLL 850
PNIEHITLKD EDQKNIQYMK LIINICGCVL SIRNVRNIRV RQPLNKITIY 900
SYNNNDLFNL PVKYQNILLD EINVKSIVFK SNIEDIASFQ LKLNFPELGK 950
RIPEKMKNLI SLLKSNQWKI LENGQLMLGI REGEHYILED NEYTLNLKVH 1000
SEFASTITLG PNLLGVLVLD NTLTDELIME GIARDIVRII QQSRKDNKFN 1050
VSDKIDVVIC TQDKMVKNSV QAWYEYIVQQ TLSLSLVIYE NLDANNVAEY 1100
CKTTMKDRNL TLFIKKL 1117
Length:1,117
Mass (Da):130,645
Last modified:December 20, 2005 - v2
Checksum:iC48E96F5C239C230
GO

Sequence cautioni

The sequence CAH58215.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAI27003.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR767821 Genomic DNA. Translation: CAH58215.1. Different initiation.
CR925678 Genomic DNA. Translation: CAI27003.1. Different initiation.
RefSeqiYP_180351.1. NC_005295.2.
YP_197385.2. NC_006832.1.

Genome annotation databases

EnsemblBacteriaiCAH58215; CAH58215; Erum4870.
CAI27003; CAI27003; ERWE_CDS_05090.
GeneIDi3233369.
3260905.
KEGGieru:Erum4870.
erw:ERWE_CDS_05090.
PATRICi20580862. VBIEhrRum92411_0543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR767821 Genomic DNA. Translation: CAH58215.1 . Different initiation.
CR925678 Genomic DNA. Translation: CAI27003.1 . Different initiation.
RefSeqi YP_180351.1. NC_005295.2.
YP_197385.2. NC_006832.1.

3D structure databases

ProteinModelPortali Q5HB43.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 254945.Erum4870.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAH58215 ; CAH58215 ; Erum4870 .
CAI27003 ; CAI27003 ; ERWE_CDS_05090 .
GeneIDi 3233369.
3260905.
KEGGi eru:Erum4870.
erw:ERWE_CDS_05090.
PATRICi 20580862. VBIEhrRum92411_0543.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci ERUM254945:GJ2L-536-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Welgevonden.
  2. "Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity."
    Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.
    J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Welgevonden.

Entry informationi

Entry nameiSYI_EHRRW
AccessioniPrimary (citable) accession number: Q5HB43
Secondary accession number(s): Q5FEI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 14, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi