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Q5HAN0 (BIOB_EHRRW) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Erum6500, ERWE_CDS_06820
OrganismEhrlichia ruminantium (strain Welgevonden) [Complete proteome] [HAMAP]
Taxonomic identifier254945 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia

Protein attributes

Sequence length322 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 322322Biotin synthase HAMAP-Rule MF_01694
PRO_0000381377

Sites

Metal binding551Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding621Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding991Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1301Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1901Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2621Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5HAN0 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: F8CB0E1BDCFB4541

FASTA32236,318
        10         20         30         40         50         60 
MCTSIRHDWQ LPEVLELFNL PFNDLILNAH LIHRKFFNSN EIQIAGLLNI KTGGCPENCK 

        70         80         90        100        110        120 
YCSQSAHYKT QLKKEDLLNI ETIKEAIKKA KVNGIDRFCF AAAWRQIRDR DIEYICNIIS 

       130        140        150        160        170        180 
LIKSENLESC ASLGMVTLEQ AKKLKTAGLD FYNHNIDTSR DFYYNVTTTR SYDDRLSSLN 

       190        200        210        220        230        240 
NISEAEINIC SGGILGLGES IEDRAKMLLT LANLKKHPKS VPINRLVPIK GTPFENNPKI 

       250        260        270        280        290        300 
SNIDFIRTIA VARILMPESY VRLAAGRESM SHEMQALCLF AGANSLFYGE KLLTTPNADC 

       310        320 
NDDKNLLSKL GVKTKQAVFF DS 

« Hide

References

[1]"The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number."
Collins N.E., Liebenberg J., de Villiers E.P., Brayton K.A., Louw E., Pretorius A., Faber F.E., van Heerden H., Josemans A., van Kleef M., Steyn H.C., van Strijp M.F., Zweygarth E., Jongejan F., Maillard J.C., Berthier D., Botha M., Joubert F. expand/collapse author list , Corton C.H., Thomson N.R., Allsopp M.T., Allsopp B.A.
Proc. Natl. Acad. Sci. U.S.A. 102:838-843(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Welgevonden.
[2]"Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity."
Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.
J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Welgevonden.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR767821 Genomic DNA. Translation: CAH58382.1.
CR925678 Genomic DNA. Translation: CAI27176.1.
RefSeqYP_180514.1. NC_005295.2.
YP_197558.1. NC_006832.1.

3D structure databases

ProteinModelPortalQ5HAN0.
SMRQ5HAN0. Positions 6-316.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING254945.Erum6500.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAH58382; CAH58382; Erum6500.
CAI27176; CAI27176; ERWE_CDS_06820.
GeneID3233288.
3260991.
KEGGeru:Erum6500.
erw:ERWE_CDS_06820.
PATRIC20581228. VBIEhrRum92411_0721.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycERUM254945:GJ2L-714-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_EHRRW
AccessionPrimary (citable) accession number: Q5HAN0
Secondary accession number(s): Q5FD99
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 15, 2005
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways