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Q5HAN0

- BIOB_EHRRW

UniProt

Q5HAN0 - BIOB_EHRRW

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Protein
Biotin synthase
Gene
bioB, Erum6500, ERWE_CDS_06820
Organism
Ehrlichia ruminantium (strain Welgevonden)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi99 – 991Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi130 – 1301Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi190 – 1901Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi262 – 2621Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciERUM254945:GJ2L-714-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Erum6500, ERWE_CDS_06820
OrganismiEhrlichia ruminantium (strain Welgevonden)
Taxonomic identifieri254945 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
ProteomesiUP000001021: Chromosome, UP000006739: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Biotin synthaseUniRule annotation
PRO_0000381377Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi254945.Erum6500.

Structurei

3D structure databases

ProteinModelPortaliQ5HAN0.
SMRiQ5HAN0. Positions 6-316.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5HAN0-1 [UniParc]FASTAAdd to Basket

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MCTSIRHDWQ LPEVLELFNL PFNDLILNAH LIHRKFFNSN EIQIAGLLNI    50
KTGGCPENCK YCSQSAHYKT QLKKEDLLNI ETIKEAIKKA KVNGIDRFCF 100
AAAWRQIRDR DIEYICNIIS LIKSENLESC ASLGMVTLEQ AKKLKTAGLD 150
FYNHNIDTSR DFYYNVTTTR SYDDRLSSLN NISEAEINIC SGGILGLGES 200
IEDRAKMLLT LANLKKHPKS VPINRLVPIK GTPFENNPKI SNIDFIRTIA 250
VARILMPESY VRLAAGRESM SHEMQALCLF AGANSLFYGE KLLTTPNADC 300
NDDKNLLSKL GVKTKQAVFF DS 322
Length:322
Mass (Da):36,318
Last modified:February 15, 2005 - v1
Checksum:iF8CB0E1BDCFB4541
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR767821 Genomic DNA. Translation: CAH58382.1.
CR925678 Genomic DNA. Translation: CAI27176.1.
RefSeqiWP_011155330.1. NC_006832.1.
YP_180514.1. NC_005295.2.
YP_197558.1. NC_006832.1.

Genome annotation databases

EnsemblBacteriaiCAH58382; CAH58382; Erum6500.
CAI27176; CAI27176; ERWE_CDS_06820.
GeneIDi3233288.
3260991.
KEGGieru:Erum6500.
erw:ERWE_CDS_06820.
PATRICi20581228. VBIEhrRum92411_0721.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR767821 Genomic DNA. Translation: CAH58382.1 .
CR925678 Genomic DNA. Translation: CAI27176.1 .
RefSeqi WP_011155330.1. NC_006832.1.
YP_180514.1. NC_005295.2.
YP_197558.1. NC_006832.1.

3D structure databases

ProteinModelPortali Q5HAN0.
SMRi Q5HAN0. Positions 6-316.
ModBasei Search...

Protein-protein interaction databases

STRINGi 254945.Erum6500.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAH58382 ; CAH58382 ; Erum6500 .
CAI27176 ; CAI27176 ; ERWE_CDS_06820 .
GeneIDi 3233288.
3260991.
KEGGi eru:Erum6500.
erw:ERWE_CDS_06820.
PATRICi 20581228. VBIEhrRum92411_0721.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci ERUM254945:GJ2L-714-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Welgevonden.
  2. "Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity."
    Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.
    J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Welgevonden.

Entry informationi

Entry nameiBIOB_EHRRW
AccessioniPrimary (citable) accession number: Q5HAN0
Secondary accession number(s): Q5FD99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 15, 2005
Last modified: September 3, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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