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Q5HAM9

- Q5HAM9_EHRRW

UniProt

Q5HAM9 - Q5HAM9_EHRRW

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Protein
Phosphoribosylformylglycinamidine synthase subunit PurL
Gene
probable purL, purL, Erum6510, ERWE_CDS_06830
Organism
Ehrlichia ruminantium (strain Welgevonden)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL By similarity.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei266 – 2661 By similarityUniRule annotation
Binding sitei322 – 3221ATP By similarityUniRule annotation
Metal bindingi324 – 3241Magnesium 1 By similarityUniRule annotation
Active sitei326 – 3261Proton acceptor By similarityUniRule annotation
Binding sitei347 – 3471Substrate By similarityUniRule annotation
Metal bindingi348 – 3481Magnesium 2 By similarityUniRule annotation
Binding sitei487 – 4871Substrate By similarityUniRule annotation
Metal bindingi516 – 5161Magnesium 2 By similarityUniRule annotation
Binding sitei747 – 7471ATP By similarityUniRule annotation
Binding sitei784 – 7841ATP; via carbonyl oxygen and amide nitrogen By similarityUniRule annotation
Binding sitei787 – 7871Substrate By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciERUM254945:GJ2L-715-MONOMER.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit II
Glutamine amidotransferase PurL
Phosphoribosylformylglycinamidine synthase subunit II
Gene namesi
Name:probable purLImported
Synonyms:purLUniRule annotationImported
Ordered Locus Names:Erum6510Imported, ERWE_CDS_06830Imported
OrganismiEhrlichia ruminantium (strain Welgevonden)Imported
Taxonomic identifieri254945 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
ProteomesiUP000001021: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi254945.Erum6510.

Structurei

3D structure databases

ProteinModelPortaliQ5HAM9.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni557 – 5593Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0046.
HOGENOMiHOG000238228.
KOiK01952.
OMAiQAVVFKI.
OrthoDBiEOG6FNHHR.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
HAMAPiMF_00420. PurL_2.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.

Sequencei

Sequence statusi: Complete.

Q5HAM9-1 [UniParc]FASTAAdd to Basket

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MIIRIGIADK TLLENYRNTT LTVYTIDTQK NLDQETINKI GTLLTNPITQ     50
RFFTFIYNNV ELNYQEVNTT NNYNLLNIFN ANWSLEKSFL PGVTDNVGNT 100
TKCMIKEALK CDFSLDVITS QVIFSQETVP QEKDILKIYN PLIEYCKLSI 150
RKETGFNVRF FGNKQILDLN KLYDNSRTHN TIFIIPYVRQ EDLVPQCITQ 200
DTCVTAVNLN IQDEELMELS KYGIHGRGGL NLSLEAMKAI QAYFKKINRN 250
PNDIELETLA QTWSEHCKHN IFSSPIDDII DGLYKHYIKR ATYEINSEIC 300
VSTFSDNAGA IIFDDNFIIA DKVETHNSPS ALDPFGGAIT GILGVNRDII 350
GFGKGAKPIL NAYYFCFSES EKELYRDKMC TVSVLPSETI INGVIKGVNS 400
GGNCSGIPTG LGSVYFDDRF QSKPLVFVGC TGIIPRIIGN TPSHLKGPAD 450
GDYIVIAGGR TGRDGIHGAT FSSNALTENN SNSTVVQIGD PITQKKLSDA 500
IIKEARDLNL YNAITDNGAG GLSSSIGEMG NNGFKVELNK VLLKHKNMLP 550
WEIWVSESQE RMTLAIPPSK FPIFEKIMKK HDVEISIIGT FNNTKKAVVS 600
YNDSIIMDID INFLHNGIPK THLKTIPWSN IISSAVDTLH NKPLDTELNE 650
MMQRMNICSK EFISTQYDHE VQGTSVIKPI QGKGRVDGEA IVIRPILSSE 700
RGLVKSHGLG SSYGEISTYH MAACAIDTAI RNYIAIGGNF HHLALLDNFC 750
WCDSTNPKRL WQLKNAAQAC YDYAKIFKTP FISGKDSMFN DFKGYNNKGE 800
PINISAPPSL LISTVGIIEN IHNAITLDVK NPGDLIYILG VTYDELGRSE 850
YQKYSGLGNN NVPQVRAKHA KKLYKLYSNA VNTNIISSAI ALNLGGLVIG 900
LIKSLIGGEL GAKIDLSLVP THNIEDNSIK EKVILFSESQ SRILVTIAPH 950
NKQKFETIFK DIAHANIGII SDTNTLIINN MHIINLNTLK HSYKKFSNMK 1000
IQAYANAEYI 1010
Length:1,010
Mass (Da):112,383
Last modified:February 15, 2005 - v1
Checksum:i5BD1D05F49D027DD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR767821 Genomic DNA. Translation: CAH58383.1.
CR925678 Genomic DNA. Translation: CAI27177.1.
RefSeqiWP_011155331.1. NC_006832.1.
YP_180515.1. NC_005295.2.
YP_197559.1. NC_006832.1.

Genome annotation databases

EnsemblBacteriaiCAH58383; CAH58383; Erum6510.
CAI27177; CAI27177; ERWE_CDS_06830.
GeneIDi3232585.
3261422.
KEGGieru:Erum6510.
erw:ERWE_CDS_06830.
PATRICi20581232. VBIEhrRum92411_0723.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR767821 Genomic DNA. Translation: CAH58383.1 .
CR925678 Genomic DNA. Translation: CAI27177.1 .
RefSeqi WP_011155331.1. NC_006832.1.
YP_180515.1. NC_005295.2.
YP_197559.1. NC_006832.1.

3D structure databases

ProteinModelPortali Q5HAM9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 254945.Erum6510.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAH58383 ; CAH58383 ; Erum6510 .
CAI27177 ; CAI27177 ; ERWE_CDS_06830 .
GeneIDi 3232585.
3261422.
KEGGi eru:Erum6510.
erw:ERWE_CDS_06830.
PATRICi 20581232. VBIEhrRum92411_0723.

Phylogenomic databases

eggNOGi COG0046.
HOGENOMi HOG000238228.
KOi K01952.
OMAi QAVVFKI.
OrthoDBi EOG6FNHHR.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00128 .
BioCyci ERUM254945:GJ2L-715-MONOMER.

Family and domain databases

Gene3Di 3.30.1330.10. 2 hits.
HAMAPi MF_00420. PurL_2.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view ]
Pfami PF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view ]
SUPFAMi SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WelgevondenImported and Welgevonden [ARC-OVI].
  2. "Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity."
    Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.
    J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Welgevonden [CIRAD].

Entry informationi

Entry nameiQ5HAM9_EHRRW
AccessioniPrimary (citable) accession number: Q5HAM9
Secondary accession number(s): Q5FD98
Entry historyi
Integrated into UniProtKB/TrEMBL: February 15, 2005
Last sequence update: February 15, 2005
Last modified: September 3, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

In Gram-negative bacteria and most eukaryotes, FGAM synthase is only formed by PurL, a single polypeptide of 140 kDa (large PurL). However in Gram-positive bacteria and archaebacteria, phosphoribosylformylglycinamidine synthase is composed of three separate proteins: PurL (small PurL), PurQ and PurS By similarity.UniRule annotation

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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