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Q5HAM9

- Q5HAM9_EHRRW

UniProt

Q5HAM9 - Q5HAM9_EHRRW

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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

probable purL

Organism
Ehrlichia ruminantium (strain Welgevonden)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei266 – 2661UniRule annotation
Binding sitei322 – 3221ATPUniRule annotation
Metal bindingi324 – 3241Magnesium 1UniRule annotation
Active sitei326 – 3261Proton acceptorUniRule annotation
Binding sitei347 – 3471SubstrateUniRule annotation
Metal bindingi348 – 3481Magnesium 2UniRule annotation
Binding sitei487 – 4871SubstrateUniRule annotation
Metal bindingi516 – 5161Magnesium 2UniRule annotation
Binding sitei747 – 7471ATPUniRule annotation
Binding sitei784 – 7841ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei787 – 7871SubstrateUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciERUM254945:GJ2L-715-MONOMER.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:probable purLImported
Synonyms:purLUniRule annotationImported
Ordered Locus Names:Erum6510Imported, ERWE_CDS_06830Imported
OrganismiEhrlichia ruminantium (strain Welgevonden)Imported
Taxonomic identifieri254945 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
ProteomesiUP000001021: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Protein-protein interaction databases

STRINGi254945.Erum6510.

Structurei

3D structure databases

ProteinModelPortaliQ5HAM9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni557 – 5593Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0046.
HOGENOMiHOG000238228.
KOiK01952.
OMAiQAVVFKI.
OrthoDBiEOG6FNHHR.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
HAMAPiMF_00420. PurL_2.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.

Sequencei

Sequence statusi: Complete.

Q5HAM9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIIRIGIADK TLLENYRNTT LTVYTIDTQK NLDQETINKI GTLLTNPITQ
60 70 80 90 100
RFFTFIYNNV ELNYQEVNTT NNYNLLNIFN ANWSLEKSFL PGVTDNVGNT
110 120 130 140 150
TKCMIKEALK CDFSLDVITS QVIFSQETVP QEKDILKIYN PLIEYCKLSI
160 170 180 190 200
RKETGFNVRF FGNKQILDLN KLYDNSRTHN TIFIIPYVRQ EDLVPQCITQ
210 220 230 240 250
DTCVTAVNLN IQDEELMELS KYGIHGRGGL NLSLEAMKAI QAYFKKINRN
260 270 280 290 300
PNDIELETLA QTWSEHCKHN IFSSPIDDII DGLYKHYIKR ATYEINSEIC
310 320 330 340 350
VSTFSDNAGA IIFDDNFIIA DKVETHNSPS ALDPFGGAIT GILGVNRDII
360 370 380 390 400
GFGKGAKPIL NAYYFCFSES EKELYRDKMC TVSVLPSETI INGVIKGVNS
410 420 430 440 450
GGNCSGIPTG LGSVYFDDRF QSKPLVFVGC TGIIPRIIGN TPSHLKGPAD
460 470 480 490 500
GDYIVIAGGR TGRDGIHGAT FSSNALTENN SNSTVVQIGD PITQKKLSDA
510 520 530 540 550
IIKEARDLNL YNAITDNGAG GLSSSIGEMG NNGFKVELNK VLLKHKNMLP
560 570 580 590 600
WEIWVSESQE RMTLAIPPSK FPIFEKIMKK HDVEISIIGT FNNTKKAVVS
610 620 630 640 650
YNDSIIMDID INFLHNGIPK THLKTIPWSN IISSAVDTLH NKPLDTELNE
660 670 680 690 700
MMQRMNICSK EFISTQYDHE VQGTSVIKPI QGKGRVDGEA IVIRPILSSE
710 720 730 740 750
RGLVKSHGLG SSYGEISTYH MAACAIDTAI RNYIAIGGNF HHLALLDNFC
760 770 780 790 800
WCDSTNPKRL WQLKNAAQAC YDYAKIFKTP FISGKDSMFN DFKGYNNKGE
810 820 830 840 850
PINISAPPSL LISTVGIIEN IHNAITLDVK NPGDLIYILG VTYDELGRSE
860 870 880 890 900
YQKYSGLGNN NVPQVRAKHA KKLYKLYSNA VNTNIISSAI ALNLGGLVIG
910 920 930 940 950
LIKSLIGGEL GAKIDLSLVP THNIEDNSIK EKVILFSESQ SRILVTIAPH
960 970 980 990 1000
NKQKFETIFK DIAHANIGII SDTNTLIINN MHIINLNTLK HSYKKFSNMK
1010
IQAYANAEYI
Length:1,010
Mass (Da):112,383
Last modified:February 15, 2005 - v1
Checksum:i5BD1D05F49D027DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR767821 Genomic DNA. Translation: CAH58383.1.
CR925678 Genomic DNA. Translation: CAI27177.1.
RefSeqiYP_180515.1. NC_005295.2.
YP_197559.1. NC_006832.1.

Genome annotation databases

EnsemblBacteriaiCAH58383; CAH58383; Erum6510.
CAI27177; CAI27177; ERWE_CDS_06830.
GeneIDi3232585.
3261422.
KEGGieru:Erum6510.
erw:ERWE_CDS_06830.
PATRICi20581232. VBIEhrRum92411_0723.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR767821 Genomic DNA. Translation: CAH58383.1 .
CR925678 Genomic DNA. Translation: CAI27177.1 .
RefSeqi YP_180515.1. NC_005295.2.
YP_197559.1. NC_006832.1.

3D structure databases

ProteinModelPortali Q5HAM9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 254945.Erum6510.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAH58383 ; CAH58383 ; Erum6510 .
CAI27177 ; CAI27177 ; ERWE_CDS_06830 .
GeneIDi 3232585.
3261422.
KEGGi eru:Erum6510.
erw:ERWE_CDS_06830.
PATRICi 20581232. VBIEhrRum92411_0723.

Phylogenomic databases

eggNOGi COG0046.
HOGENOMi HOG000238228.
KOi K01952.
OMAi QAVVFKI.
OrthoDBi EOG6FNHHR.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00128 .
BioCyci ERUM254945:GJ2L-715-MONOMER.

Family and domain databases

Gene3Di 3.30.1330.10. 2 hits.
HAMAPi MF_00420. PurL_2.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view ]
Pfami PF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view ]
SUPFAMi SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WelgevondenImported and Welgevonden [ARC-OVI]Imported.
  2. "Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity."
    Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.
    J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Welgevonden [CIRAD]Imported.

Entry informationi

Entry nameiQ5HAM9_EHRRW
AccessioniPrimary (citable) accession number: Q5HAM9
Secondary accession number(s): Q5FD98
Entry historyi
Integrated into UniProtKB/TrEMBL: February 15, 2005
Last sequence update: February 15, 2005
Last modified: November 26, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3