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Q5HAL7 (Q5HAL7_EHRRW) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutathione synthetase HAMAP-Rule MF_00162
EC=6.3.2.3 HAMAP-Rule MF_00162
Alternative name(s):
GSH synthetase HAMAP-Rule MF_00162
Glutathione synthase HAMAP-Rule MF_00162
Gene names
Name:gshB HAMAP-Rule MF_00162 EMBL CAH58396.1
Ordered Locus Names:Erum6640, ERWE_CDS_06960
OrganismEhrlichia ruminantium (strain Welgevonden) [Complete proteome] [HAMAP]
Taxonomic identifier254945 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Ehrlichia ›

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds (magnesium or manganese,ion) per subunit By similarity. HAMAP-Rule MF_00162

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family. HAMAP-Rule MF_00162

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00162

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain119 – 302184ATP-grasp By similarity HAMAP-Rule MF_00162
Nucleotide binding145 – 20056ATP By similarity HAMAP-Rule MF_00162

Sites

Metal binding2731Magnesium or manganese By similarity HAMAP-Rule MF_00162
Metal binding2751Magnesium or manganese By similarity HAMAP-Rule MF_00162

Sequences

Sequence LengthMass (Da)Tools
Q5HAL7 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 17652D7D097A51E3

FASTA31235,914
        10         20         30         40         50         60 
MALVVAFQMD RDVVVGKDVS VKFIQEAQIR GHTVFFFTPD KLAIKNNIPV ASACLVTVEQ 

        70         80         90        100        110        120 
EKLKFENVQE LFLEDMDIIF IRQNPPFDMR YITSTYILEK LTKPLVINDP KFLRDYPEKL 

       130        140        150        160        170        180 
LPLLFDDFIP DTLITEDYSM LQNFYYEHKD IILKPLYSYG GNDVIRINNN VDIKVIVALM 

       190        200        210        220        230        240 
IEKYKCPIIA QQFIPNINND KRILLLDGKP IGVFRRRILS AGEVRTNLRV GSVAEPAELS 

       250        260        270        280        290        300 
DRDYNICLAI EDKLALKGLI FAGIDVLDGY LIEINVTSPC GVLEVNKLYN VCLEKKCWDC 

       310 
FEKKFYARCG YV

« Hide

References

[1]"The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number."
Collins N.E., Liebenberg J., de Villiers E.P., Brayton K.A., Louw E., Pretorius A., Faber F.E., van Heerden H., Josemans A., van Kleef M., Steyn H.C., van Strijp M.F., Zweygarth E., Jongejan F., Maillard J.C., Berthier D., Botha M., Joubert F. expand/collapse author list , Corton C.H., Thomson N.R., Allsopp M.T., Allsopp B.A.
Proc. Natl. Acad. Sci. U.S.A. 102:838-843(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Welgevonden [ARC-OVI].
[2]"Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity."
Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.
J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Welgevonden [CIRAD].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CR767821 Genomic DNA. Translation: CAH58396.1.
CR925678 Genomic DNA. Translation: CAI27190.1.
RefSeqYP_180527.1. NC_005295.2.
YP_197572.1. NC_006832.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING254945.Erum6640.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAH58396; CAH58396; Erum6640.
CAI27190; CAI27190; ERWE_CDS_06960.
GeneID3233352.
3260875.
KEGGeru:Erum6640.
erw:ERWE_CDS_06960.
PATRIC20581260. VBIEhrRum92411_0737.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMAWMRKDPP.
ProtClustDBPRK05246.

Enzyme and pathway databases

BioCycERUM254945:GJ2L-728-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ5HAL7_EHRRW
AccessionPrimary (citable) accession number: Q5HAL7
Secondary accession number(s): Q5FDC9
Entry history
Integrated into UniProtKB/TrEMBL: February 15, 2005
Last sequence update: February 15, 2005
Last modified: May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info