Q5HAK7 (KGUA_EHRRW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanylate kinase
EC=2.7.4.8

Alternative name(s):
GMP kinase

Gene names

Name:	gmk
Ordered Locus Names:	Erum6740, ERWE_CDS_07070

Organism

Ehrlichia ruminantium (strain Welgevonden) [Complete proteome] [HAMAP]

Taxonomic identifier

254945 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Ehrlichia › canis group › Ehrlichia ruminantium

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential for recycling GMP and indirectly, cGMP By similarity. HAMAP-Rule MF_00328
Catalytic activity	ATP + GMP = ADP + GDP. HAMAP-Rule MF_00328
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the guanylate kinase family. Contains 1 guanylate kinase-like domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	purine nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW guanylate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Guanylate kinase HAMAP-Rule MF_00328		PRO_0000266322
Regions
	Domain	9 – 188	180	Guanylate kinase-like
	Nucleotide binding	16 – 23	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HAK7 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 31C2FBBFAD2B49B6	FASTA	209	24,376
	10 20 30 40 50 60 MNNNLKSRGI MLVMSSPSGG GKTTISQLLV NELQGEIIRS VSVTTREPRN EEVEGKDYFF 70 80 90 100 110 120 VTEDEFHHLC NTNQMLEYAK VFGNYYGIPR RFVMDNINNG ISILFSIDWQ GAFKLIDIMS 130 140 150 160 170 180 EHVVSVFILP PSMEELKRRL YNRSGESDMI NQRLKEAAFE ISHCYRYNYI IVNHNIEESV 190 200 QQIKSIFIAE KLKTHRKMFL EQVVKSYYI

References

[1]

"The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number." Collins N.E., Liebenberg J., de Villiers E.P., Brayton K.A., Louw E., Pretorius A., Faber F.E., van Heerden H., Josemans A., van Kleef M., Steyn H.C., van Strijp M.F., Zweygarth E., Jongejan F., Maillard J.C., Berthier D., Botha M., Joubert F. , Corton C.H., Thomson N.R., Allsopp M.T., Allsopp B.A. Proc. Natl. Acad. Sci. U.S.A. 102:838-843(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Welgevonden.

[2]

"Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity." Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D. J. Bacteriol. 188:2533-2542(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Welgevonden.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR767821 Genomic DNA. Translation: CAH58406.1. CR925678 Genomic DNA. Translation: CAI27201.1.
RefSeq	YP_180537.1. NC_005295.2. YP_197583.1. NC_006832.1.
3D structure databases
HSSP	HSSP built from PDB template 2AN9 based on UniProtKB P60546.
ProteinModelPortal	Q5HAK7.
ModBase	Search...
Protein-protein interaction databases
STRING	254945.Erum6740.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAH58406; CAH58406; Erum6740. CAI27201; CAI27201; ERWE_CDS_07070.
GeneID	3233345. 3260863.
KEGG	eru:Erum6740. erw:ERWE_CDS_07070.
PATRIC	20581288. VBIEhrRum92411_0750.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0194.
HOGENOM	HOG000037638.
KO	K00942.
OMA	FVIINEL.
ProtClustDB	PRK00300.
Enzyme and pathway databases
BioCyc	ERUM254945:GJ2L-740-MONOMER.
Family and domain databases
HAMAP	MF_00328. Guanylate_kinase.
InterPro	IPR008144. Guanylate_kin. IPR008145. Guanylate_kin/L-typ_Ca_channel. IPR020590. Guanylate_kinase_CS. IPR017665. Guanylate_kinase_sub. [Graphical view]
Pfam	PF00625. Guanylate_kin. 1 hit. [Graphical view]
SMART	SM00072. GuKc. 1 hit. [Graphical view]
TIGRFAMs	TIGR03263. guanyl_kin. 1 hit.
PROSITE	PS00856. GUANYLATE_KINASE_1. 1 hit. PS50052. GUANYLATE_KINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KGUA_EHRRW

Accession

Primary (citable) accession number: Q5HAK7
Secondary accession number(s): Q5FDG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program