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Protein

Ribonuclease 3

Gene

rnc

Organism
Ehrlichia ruminantium (strain Welgevonden)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43MagnesiumUniRule annotation1
Active sitei47UniRule annotation1
Metal bindingi116MagnesiumUniRule annotation1
Active sitei119UniRule annotation1
Metal bindingi119MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, RNA-binding, rRNA-binding
Biological processmRNA processing, rRNA processing, tRNA processing
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
Alternative name(s):
Ribonuclease IIIUniRule annotation
Short name:
RNase IIIUniRule annotation
Gene namesi
Name:rncUniRule annotation
Ordered Locus Names:Erum8070, ERWE_CDS_08540
OrganismiEhrlichia ruminantium (strain Welgevonden)
Taxonomic identifieri254945 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
Proteomesi
  • UP000001021 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002285291 – 227Ribonuclease 3Add BLAST227

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi254945.ERWE_CDS_08540.

Structurei

3D structure databases

ProteinModelPortaliQ5HA74.
SMRiQ5HA74.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 130RNase IIIUniRule annotationAdd BLAST128
Domaini155 – 224DRBMUniRule annotationAdd BLAST70

Sequence similaritiesi

Belongs to the ribonuclease III family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108ZBM. Bacteria.
COG0571. LUCA.
HOGENOMiHOG000246809.
KOiK03685.
OMAiLTHKSCK.
OrthoDBiPOG091H00R1.

Family and domain databases

CDDicd00593. RIBOc. 1 hit.
Gene3Di1.10.1520.10. 1 hit.
HAMAPiMF_00104. RNase_III. 1 hit.
InterProiView protein in InterPro
IPR014720. dsRBD_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
PfamiView protein in Pfam
PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
SMARTiView protein in SMART
SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiView protein in PROSITE
PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5HA74-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITNAISKII KYDFKNTQLL NEALTHPSVL SKDNNNFNYE RLEFLGDAVL
60 70 80 90 100
NLVISEMLFN IFPYDTEGNL AKKKTALVCG TKLVEIAQSI NLGIFIIMSD
110 120 130 140 150
GERSCGGANN SNNLENALEA LIGAIYLDGG LKAAKDFIFL FWKNSATHMK
160 170 180 190 200
VPPQDAKTIL QEWAQSKGFP APSYHIINKS GPDHNPCFTV EVRINSHETL
210 220
HATGHNKKLA EQKAASLMLA KINYKIK
Length:227
Mass (Da):25,182
Last modified:February 15, 2005 - v1
Checksum:i820424B1FF6D71CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR767821 Genomic DNA. Translation: CAH58541.1.
CR925678 Genomic DNA. Translation: CAI27348.1.
RefSeqiWP_011155486.1. NC_006832.1.

Genome annotation databases

EnsemblBacteriaiCAH58541; CAH58541; Erum8070.
CAI27348; CAI27348; ERWE_CDS_08540.
KEGGieru:Erum8070.
erw:ERWE_CDS_08540.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRNC_EHRRW
AccessioniPrimary (citable) accession number: Q5HA74
Secondary accession number(s): Q5FE00
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: February 15, 2005
Last modified: June 7, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families