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Protein

Transcription initiation factor TFIID subunit 7-like

Gene

TAF7L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probably functions as a spermatogensis-specific component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in spermatogenesis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 7-like
Alternative name(s):
Cancer/testis antigen 40
Short name:
CT40
RNA polymerase II TBP-associated factor subunit Q
TATA box-binding protein-associated factor 50 kDa
Transcription initiation factor TFIID 50 kDa subunit
Gene namesi
Name:TAF7L
Synonyms:TAF2Q
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:11548. TAF7L.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Cytoplasmic in spermatogonia and early spermatocytes (preleptotene, leptotene, and zygotene); translocates into the nuclei of pachytene spermatocytes and round spermatids.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36323.

Polymorphism and mutation databases

BioMutaiTAF7L.
DMDMi74762183.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Transcription initiation factor TFIID subunit 7-likePRO_0000307861Add
BLAST

Proteomic databases

MaxQBiQ5H9L4.
PaxDbiQ5H9L4.
PRIDEiQ5H9L4.

PTM databases

PhosphoSiteiQ5H9L4.

Expressioni

Tissue specificityi

Testis-specific.1 Publication

Gene expression databases

BgeeiQ5H9L4.
CleanExiHS_TAF7L.
ExpressionAtlasiQ5H9L4. baseline and differential.
GenevisibleiQ5H9L4. HS.

Organism-specific databases

HPAiHPA001879.

Interactioni

Subunit structurei

TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TAF7L may replace TAF7 in a spermatogenesis-specific form of TFIID. Interacts with TBP; the interaction occurs in a sub-population of cells (pachytene and haploid round spermatids) and is developmentally regulated through differential intracellular localization of the two proteins. Interacts with TAF1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi119964. 5 interactions.
IntActiQ5H9L4. 2 interactions.
STRINGi9606.ENSP00000361998.

Structurei

3D structure databases

ProteinModelPortaliQ5H9L4.
SMRiQ5H9L4. Positions 97-283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili342 – 462121Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi336 – 37742Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the TAF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5414.
GeneTreeiENSGT00390000010815.
HOGENOMiHOG000035121.
HOVERGENiHBG013606.
InParanoidiQ5H9L4.
KOiK03132.
OMAiSVKMKDK.
OrthoDBiEOG7GJ6FT.
PhylomeDBiQ5H9L4.
TreeFamiTF313044.

Family and domain databases

InterProiIPR006751. TAFII55_prot_cons_reg.
[Graphical view]
PfamiPF04658. TAFII55_N. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5H9L4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MECPEGQLPI SSENDSTPTV STSEVTSQQE PQILVDRGSE TTYESSADIA
60 70 80 90 100
GDEGTQIPAD EDTQTDADSS AQAAAQAPEN FQEGKDMSES QDEVPDEVEN
110 120 130 140 150
QFILRLPLEH ACTVRNLARS QSVKMKDKLK IDLLPDGRHA VVEVEDVPLA
160 170 180 190 200
AKLVDLPCVI ESLRTLDKKT FYKTADISQM LVCTADGDIH LSPEEPAAST
210 220 230 240 250
DPNIVRKKER GREEKCVWKH GITPPLKNVR KKRFRKTQKK VPDVKEMEKS
260 270 280 290 300
SFTEYIESPD VENEVKRLLR SDAEAVSTRW EVIAEDGTKE IESQGSIPGF
310 320 330 340 350
LISSGMSSHK QGHTSSEYDM LREMFSDSRS NNDDDEDEDD EDEDEDEDED
360 370 380 390 400
EDEDKEEEEE DCSEEYLERQ LQAEFIESGQ YRANEGTSSI VMEIQKQIEK
410 420 430 440 450
KEKKLHKIQN KAQRQKDLIM KVENLTLKNH FQSVLEQLEL QEKQKNEKLI
460
SLQEQLQRFL KK
Length:462
Mass (Da):52,588
Last modified:February 15, 2005 - v1
Checksum:i92BB59377D35A62F
GO
Isoform 2 (identifier: Q5H9L4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-86: Missing.

Note: No experimental confirmation available.
Show »
Length:376
Mass (Da):43,570
Checksum:i4D5BDFC2013DFE63
GO
Isoform 3 (identifier: Q5H9L4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-86: Missing.
     317-390: Missing.

Note: No experimental confirmation available.
Show »
Length:302
Mass (Da):34,726
Checksum:i03FDA44019BFAB5A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti34 – 341L → P.1 Publication
Corresponds to variant rs5951328 [ dbSNP | Ensembl ].
VAR_036695
Natural varianti61 – 611E → K.1 Publication
Corresponds to variant rs147493489 [ dbSNP | Ensembl ].
VAR_036696
Natural varianti308 – 3081S → G.2 Publications
Corresponds to variant rs35899692 [ dbSNP | Ensembl ].
VAR_036697
Natural varianti350 – 3512Missing .3 Publications
VAR_036698
Natural varianti458 – 4581R → H.2 Publications
Corresponds to variant rs41310729 [ dbSNP | Ensembl ].
VAR_036699

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8686Missing in isoform 2 and isoform 3. 2 PublicationsVSP_028847Add
BLAST
Alternative sequencei317 – 39074Missing in isoform 3. 1 PublicationVSP_028848Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF285595 mRNA. Translation: AAK31974.1.
AK026810 mRNA. Translation: BAB15560.1.
Z68331, AL109801 Genomic DNA. Translation: CAI42014.1.
Z68331, AL109801 Genomic DNA. Translation: CAI42015.1.
Z68331, AL109801 Genomic DNA. Translation: CAI42017.1.
AL109801, Z68331 Genomic DNA. Translation: CAI43034.1.
AL109801, Z68331 Genomic DNA. Translation: CAI43035.1.
AL109801, Z68331 Genomic DNA. Translation: CAI43036.1.
CH471115 Genomic DNA. Translation: EAX02847.1.
CH471115 Genomic DNA. Translation: EAX02848.1.
BC043391 mRNA. Translation: AAH43391.1.
CCDSiCCDS35347.1. [Q5H9L4-1]
CCDS55466.1. [Q5H9L4-2]
RefSeqiNP_001161946.1. NM_001168474.1. [Q5H9L4-2]
NP_079161.3. NM_024885.3. [Q5H9L4-1]
UniGeneiHs.223806.

Genome annotation databases

EnsembliENST00000324762; ENSP00000320283; ENSG00000102387. [Q5H9L4-3]
ENST00000356784; ENSP00000349235; ENSG00000102387. [Q5H9L4-2]
ENST00000372907; ENSP00000361998; ENSG00000102387. [Q5H9L4-1]
GeneIDi54457.
KEGGihsa:54457.
UCSCiuc004eha.3. human. [Q5H9L4-3]
uc004ehb.3. human. [Q5H9L4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF285595 mRNA. Translation: AAK31974.1.
AK026810 mRNA. Translation: BAB15560.1.
Z68331, AL109801 Genomic DNA. Translation: CAI42014.1.
Z68331, AL109801 Genomic DNA. Translation: CAI42015.1.
Z68331, AL109801 Genomic DNA. Translation: CAI42017.1.
AL109801, Z68331 Genomic DNA. Translation: CAI43034.1.
AL109801, Z68331 Genomic DNA. Translation: CAI43035.1.
AL109801, Z68331 Genomic DNA. Translation: CAI43036.1.
CH471115 Genomic DNA. Translation: EAX02847.1.
CH471115 Genomic DNA. Translation: EAX02848.1.
BC043391 mRNA. Translation: AAH43391.1.
CCDSiCCDS35347.1. [Q5H9L4-1]
CCDS55466.1. [Q5H9L4-2]
RefSeqiNP_001161946.1. NM_001168474.1. [Q5H9L4-2]
NP_079161.3. NM_024885.3. [Q5H9L4-1]
UniGeneiHs.223806.

3D structure databases

ProteinModelPortaliQ5H9L4.
SMRiQ5H9L4. Positions 97-283.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119964. 5 interactions.
IntActiQ5H9L4. 2 interactions.
STRINGi9606.ENSP00000361998.

PTM databases

PhosphoSiteiQ5H9L4.

Polymorphism and mutation databases

BioMutaiTAF7L.
DMDMi74762183.

Proteomic databases

MaxQBiQ5H9L4.
PaxDbiQ5H9L4.
PRIDEiQ5H9L4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000324762; ENSP00000320283; ENSG00000102387. [Q5H9L4-3]
ENST00000356784; ENSP00000349235; ENSG00000102387. [Q5H9L4-2]
ENST00000372907; ENSP00000361998; ENSG00000102387. [Q5H9L4-1]
GeneIDi54457.
KEGGihsa:54457.
UCSCiuc004eha.3. human. [Q5H9L4-3]
uc004ehb.3. human. [Q5H9L4-1]

Organism-specific databases

CTDi54457.
GeneCardsiGC0XM100523.
HGNCiHGNC:11548. TAF7L.
HPAiHPA001879.
MIMi300314. gene.
neXtProtiNX_Q5H9L4.
PharmGKBiPA36323.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5414.
GeneTreeiENSGT00390000010815.
HOGENOMiHOG000035121.
HOVERGENiHBG013606.
InParanoidiQ5H9L4.
KOiK03132.
OMAiSVKMKDK.
OrthoDBiEOG7GJ6FT.
PhylomeDBiQ5H9L4.
TreeFamiTF313044.

Miscellaneous databases

GenomeRNAii54457.
NextBioi56701.
PROiQ5H9L4.
SOURCEiSearch...

Gene expression databases

BgeeiQ5H9L4.
CleanExiHS_TAF7L.
ExpressionAtlasiQ5H9L4. baseline and differential.
GenevisibleiQ5H9L4. HS.

Family and domain databases

InterProiIPR006751. TAFII55_prot_cons_reg.
[Graphical view]
PfamiPF04658. TAFII55_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An abundance of X-linked genes expressed in spermatogonia."
    Wang P.J., McCarrey J.R., Yang F., Page D.C.
    Nat. Genet. 27:422-426(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, VARIANT 350-ASP-GLU-351 DEL.
    Tissue: Testis.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Lung.
  3. "The DNA sequence of the human X chromosome."
    Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
    , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
    Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT PRO-34.
    Tissue: Eye.
  6. "The role of the testis-specific gene hTAF7L in the aetiology of male infertility."
    Stouffs K., Willems A., Lissens W., Tournaye H., Van Steirteghem A., Liebaers I.
    Mol. Hum. Reprod. 12:263-267(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS LYS-61; GLY-308; 350-ASP-GLU-351 DEL AND HIS-458.
  7. "Mutation analysis of the X-chromosome linked, testis-specific TAF7L gene in spermatogenic failure."
    Akinloye O., Gromoll J., Callies C., Nieschlag E., Simoni M.
    Andrologia 39:190-195(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS GLY-308; 350-ASP-GLU-351 DEL AND HIS-458.

Entry informationi

Entry nameiTAF7L_HUMAN
AccessioniPrimary (citable) accession number: Q5H9L4
Secondary accession number(s): Q5H9L6
, Q86XI4, Q9BXU5, Q9H5R0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: February 15, 2005
Last modified: June 24, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.