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Q5H9I0

- TFDP3_HUMAN

UniProt

Q5H9I0 - TFDP3_HUMAN

Protein

Transcription factor Dp family member 3

Gene

TFDP3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Competitive inhibitor of E2F-mediated transactivation activity. Impairs E2F-mediated cell-cycle progression from G1 to S phase.3 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei121 – 1211Critical for repression of E2F activity
    Sitei130 – 1301Critical for repression of E2F activity
    Sitei131 – 1311Critical for repression of E2F activity
    Sitei140 – 1401Critical for repression of E2F activity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi108 – 19083Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein heterodimerization activity Source: UniProtKB
    3. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: UniProtKB
    2. G1/S transition of mitotic cell cycle Source: UniProtKB
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor Dp family member 3
    Alternative name(s):
    Cancer/testis antigen 30
    Short name:
    CT30
    Hepatocellular carcinoma-associated antigen 661
    Gene namesi
    Name:TFDP3
    Synonyms:DP4, HCA661
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome X

    Organism-specific databases

    HGNCiHGNC:24603. TFDP3.

    Subcellular locationi

    Nucleus. Cytoplasm
    Note: Translocates to the nucleus on heterodimerization with E2F family members.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB-SubCell
    3. transcription factor complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi112 – 1121C → R: No effect on down-regulation of E2F transcriptional activity; when associated with or without R-161 or with V-164.
    Mutagenesisi121 – 1211T → K: Restores enhanced E2F-mediated transcriptional activity; when associated with Y-130; N-131 and E-145. 1 Publication
    Mutagenesisi130 – 1301C → Y: Restores enhanced E2F-mediated transcriptional activity; when associated with K-121; N-131 and E-145. 1 Publication
    Mutagenesisi131 – 1311Q → N: Restores enhanced E2F-mediated transcriptional activity; when associated with K-121; Y-130 and E-145. 1 Publication
    Mutagenesisi140 – 1401K → E: Restores enhanced E2F-mediated transcriptional activity; when associated with K-121; Y-130 and N-131. 1 Publication
    Mutagenesisi161 – 1611K → R: No effect on down-regulation of E2F transcriptional activity; when associated with or without R-112.
    Mutagenesisi164 – 1641T → V: No effect on down-regulation of E2F transcriptional activity; when associated R-112.

    Organism-specific databases

    PharmGKBiPA134898258.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 405405Transcription factor Dp family member 3PRO_0000305940Add
    BLAST

    Proteomic databases

    PaxDbiQ5H9I0.
    PRIDEiQ5H9I0.

    PTM databases

    PhosphoSiteiQ5H9I0.

    Expressioni

    Tissue specificityi

    Predominantly expressed in testis. Low level of expression in pancreas. Highly expressed in ovarian and colon cancer cell lines.2 Publications

    Inductioni

    In response to DNA damage.1 Publication

    Gene expression databases

    BgeeiQ5H9I0.
    CleanExiHS_TFDP3.
    GenevestigatoriQ5H9I0.

    Interactioni

    Subunit structurei

    Heterodimer: with E2F family members. TFDP3/E2F heterodimers do not bind DNA and repress E2F-dependent transcriptional activity.2 Publications

    Protein-protein interaction databases

    BioGridi119423. 2 interactions.
    IntActiQ5H9I0. 11 interactions.
    STRINGi9606.ENSP00000385461.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5H9I0.
    SMRiQ5H9I0. Positions 108-189, 194-341.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni175 – 306132Involved in negatively regulating E2F activityAdd
    BLAST
    Regioni209 – 24133DCB1By similarityAdd
    BLAST
    Regioni254 – 31057DCB2By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi156 – 19035DEF boxBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi386 – 40217Asp/Glu-rich (acidic; NCB domain)Add
    BLAST

    Domaini

    The potential DNA-binding domain differs in sequence from that of other DP family members and can not bind DNA.

    Sequence similaritiesi

    Belongs to the E2F/DP family.Curated

    Phylogenomic databases

    eggNOGiNOG282881.
    HOGENOMiHOG000030696.
    HOVERGENiHBG009894.
    InParanoidiQ5H9I0.
    KOiK09393.
    OMAiNIKRRTY.
    OrthoDBiEOG7C2R27.
    PhylomeDBiQ5H9I0.
    TreeFamiTF314396.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR003316. E2F_TDP.
    IPR028315. TFDP3.
    IPR014889. Transc_factor_DP_C.
    IPR015648. Transcrpt_fac_DP.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR12548. PTHR12548. 1 hit.
    PTHR12548:SF13. PTHR12548:SF13. 1 hit.
    PfamiPF08781. DP. 1 hit.
    PF02319. E2F_TDP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF009404. Transcription_factor_DP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5H9I0-1 [UniParc]FASTAAdd to Basket

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    MAKYVSLTEA NEELKVLMDE NQTSRPVAVH TSTVNPLGKQ LLPKTFGQSS    50
    VNIDQQVVIG MPQRPAASNI PVVGSPNPPS THFASQNQHS YSSPPWAGQH 100
    NRKGEKNGMG LCRLSMKVWE TVQRKGTTSC QEVVGELVAK FRAASNHASP 150
    NESAYDVKNI KRRTYDALNV LMAMNIISRE KKKIKWIGLT TNSAQNCQNL 200
    RVERQKRLER IKQKQSELQQ LILQQIAFKN LVLRNQYVEE QVSQRPLPNS 250
    VIHVPFIIIS SSKKTVINCS ISDDKSEYLF KFNSSFEIHD DTEVLMWMGM 300
    TFGLESGSCS AEDLKMARNL VPKALEPYVT EMAQGTFGGV FTTAGSRSNG 350
    TWLSASDLTN IAIGMLATSS GGSQYSGSRV ETPAVEEEEE EDNNDDDLSE 400
    NDEDD 405
    Length:405
    Mass (Da):44,967
    Last modified:February 15, 2005 - v1
    Checksum:iAE6F5709FE7E4C03
    GO

    Sequence cautioni

    The sequence AAF37562.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF219119 mRNA. Translation: AAF37562.2. Different initiation.
    Z77249 Genomic DNA. Translation: CAI42694.1.
    CH471107 Genomic DNA. Translation: EAX11773.1.
    CCDSiCCDS14636.2.
    RefSeqiNP_057605.3. NM_016521.2.
    UniGeneiHs.142908.

    Genome annotation databases

    EnsembliENST00000310125; ENSP00000385461; ENSG00000183434.
    GeneIDi51270.
    KEGGihsa:51270.
    UCSCiuc004exb.1. human.

    Polymorphism databases

    DMDMi74762180.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF219119 mRNA. Translation: AAF37562.2 . Different initiation.
    Z77249 Genomic DNA. Translation: CAI42694.1 .
    CH471107 Genomic DNA. Translation: EAX11773.1 .
    CCDSi CCDS14636.2.
    RefSeqi NP_057605.3. NM_016521.2.
    UniGenei Hs.142908.

    3D structure databases

    ProteinModelPortali Q5H9I0.
    SMRi Q5H9I0. Positions 108-189, 194-341.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119423. 2 interactions.
    IntActi Q5H9I0. 11 interactions.
    STRINGi 9606.ENSP00000385461.

    PTM databases

    PhosphoSitei Q5H9I0.

    Polymorphism databases

    DMDMi 74762180.

    Proteomic databases

    PaxDbi Q5H9I0.
    PRIDEi Q5H9I0.

    Protocols and materials databases

    DNASUi 51270.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000310125 ; ENSP00000385461 ; ENSG00000183434 .
    GeneIDi 51270.
    KEGGi hsa:51270.
    UCSCi uc004exb.1. human.

    Organism-specific databases

    CTDi 51270.
    GeneCardsi GC0XM132350.
    HGNCi HGNC:24603. TFDP3.
    MIMi 300772. gene.
    neXtProti NX_Q5H9I0.
    PharmGKBi PA134898258.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG282881.
    HOGENOMi HOG000030696.
    HOVERGENi HBG009894.
    InParanoidi Q5H9I0.
    KOi K09393.
    OMAi NIKRRTY.
    OrthoDBi EOG7C2R27.
    PhylomeDBi Q5H9I0.
    TreeFami TF314396.

    Miscellaneous databases

    GenomeRNAii 51270.
    NextBioi 54467.
    PROi Q5H9I0.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q5H9I0.
    CleanExi HS_TFDP3.
    Genevestigatori Q5H9I0.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR003316. E2F_TDP.
    IPR028315. TFDP3.
    IPR014889. Transc_factor_DP_C.
    IPR015648. Transcrpt_fac_DP.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR12548. PTHR12548. 1 hit.
    PTHR12548:SF13. PTHR12548:SF13. 1 hit.
    Pfami PF08781. DP. 1 hit.
    PF02319. E2F_TDP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF009404. Transcription_factor_DP. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies."
      Wang Y., Han K.-J., Pang X.-W., Vaughan H.A., Qu W., Dong X.-Y., Peng J.-R., Zhao H.-T., Rui J.-A., Leng X.-S., Cebon J., Burgess A.W., Chen W.-F.
      J. Immunol. 169:1102-1109(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.
      Tissue: Hepatoma.
    2. "The DNA sequence of the human X chromosome."
      Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
      , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
      Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    5. "Human TFDP3, a novel DP protein, inhibits DNA binding and transactivation by E2F."
      Qiao H., Di Stefano L., Tian C., Li Y.Y., Yin Y.H., Qian X.P., Pang X.W., Li Y., McNutt M.A., Helin K., Zhang Y., Chen W.F.
      J. Biol. Chem. 282:454-466(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, MUTAGENESIS OF THR-121; CYS-130; GLN-131 AND LYS-140.
    6. "E2F-1 regulation by an unusual DNA damage-responsive DP partner subunit."
      Ingram L., Munro S., Coutts A.S., La Thangue N.B.
      Cell Death Differ. 18:122-132(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INDUCTION.

    Entry informationi

    Entry nameiTFDP3_HUMAN
    AccessioniPrimary (citable) accession number: Q5H9I0
    Secondary accession number(s): Q6DK49, Q9NZ54
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3