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Q5H879

- COMT_HORSE

UniProt

Q5H879 - COMT_HORSE

Protein

Catechol O-methyltransferase

Gene

COMT

Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol By similarity.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei89 – 891S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
    Binding sitei111 – 1111S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei119 – 1191S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei137 – 1371S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei138 – 1381S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
    Binding sitei166 – 1661S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
    Metal bindingi188 – 1881MagnesiumBy similarity
    Binding sitei188 – 1881S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei191 – 1911SubstrateBy similarity
    Metal bindingi216 – 2161MagnesiumBy similarity
    Metal bindingi217 – 2171MagnesiumBy similarity
    Binding sitei217 – 2171SubstrateBy similarity
    Binding sitei246 – 2461SubstrateBy similarity

    GO - Molecular functioni

    1. catechol O-methyltransferase activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. catecholamine metabolic process Source: UniProtKB-KW
    2. neurotransmitter catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Catecholamine metabolism, Neurotransmitter degradation

    Keywords - Ligandi

    Magnesium, Metal-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Catechol O-methyltransferase (EC:2.1.1.6)
    Gene namesi
    Name:COMT
    OrganismiEquus caballus (Horse)
    Taxonomic identifieri9796 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
    ProteomesiUP000002281: Unplaced

    Subcellular locationi

    Isoform 2 : Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 269269Catechol O-methyltransferasePRO_0000318147Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ5H879.
    SMRiQ5H879. Positions 49-263.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei5 – 2521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni164 – 1674S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4122.
    HOGENOMiHOG000046392.
    HOVERGENiHBG005376.
    InParanoidiQ5H879.
    KOiK00545.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR025782. Catechol_O-MeTrfase.
    IPR017128. Catechol_O-MeTrfase_euk.
    IPR002935. O-MeTrfase_3.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10509. PTHR10509. 1 hit.
    PfamiPF01596. Methyltransf_3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51682. SAM_OMT_I. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform 1 (identifier: Q5H879-1) [UniParc]FASTAAdd to Basket

    Also known as: Membrane-bound, MB-COMT

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MLEPTPMLLA AFSLGLALLP LLFFLRRWGW LLIGWNECIL QPIHNLLMGD    50
    SKEQRILRHV LQHAVAGDPQ SVLETIDAYC SQKEWAMNVG DKKGQFLDAV 100
    VQEQQPSVLL ELGAYCGYSA VRMARLLPPG ARLLTIEINP DYAAITQRML 150
    DFAGLQDRVT VVLGASQDII PQLKKKYDVD TLDVVFLDHW KDRYLPDTLL 200
    LEECGLLRKG TVLLADNVIV PGAPDFLAHV RGSGRFECTH FSSYLEYSLW 250
    VVDGVEKAVY LGPGSPAQP 269
    Length:269
    Mass (Da):30,028
    Last modified:March 1, 2005 - v1
    Checksum:i73A24585C6768C44
    GO
    Isoform 2 (identifier: Q5H879-2) [UniParc]FASTAAdd to Basket

    Also known as: Soluble, S-COMT

    The sequence of this isoform differs from the canonical sequence as follows:
         1-47: Missing.

    Show »
    Length:222
    Mass (Da):24,600
    Checksum:i4E3097D0491A0103
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti120 – 1201A → T.1 Publication

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4747Missing in isoform 2. CuratedVSP_031176Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB178284 mRNA. Translation: BAD89294.1.
    AB178284 mRNA. Translation: BAD89295.1.
    RefSeqiNP_001075303.1. NM_001081834.1. [Q5H879-1]
    NP_001108007.1. NM_001114535.1. [Q5H879-2]
    UniGeneiEca.21379.

    Genome annotation databases

    GeneIDi100146509.
    KEGGiecb:100146509.

    Keywords - Coding sequence diversityi

    Alternative initiation, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB178284 mRNA. Translation: BAD89294.1 .
    AB178284 mRNA. Translation: BAD89295.1 .
    RefSeqi NP_001075303.1. NM_001081834.1. [Q5H879-1 ]
    NP_001108007.1. NM_001114535.1. [Q5H879-2 ]
    UniGenei Eca.21379.

    3D structure databases

    ProteinModelPortali Q5H879.
    SMRi Q5H879. Positions 49-263.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100146509.
    KEGGi ecb:100146509.

    Organism-specific databases

    CTDi 1312.

    Phylogenomic databases

    eggNOGi COG4122.
    HOGENOMi HOG000046392.
    HOVERGENi HBG005376.
    InParanoidi Q5H879.
    KOi K00545.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR025782. Catechol_O-MeTrfase.
    IPR017128. Catechol_O-MeTrfase_euk.
    IPR002935. O-MeTrfase_3.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10509. PTHR10509. 1 hit.
    Pfami PF01596. Methyltransf_3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51682. SAM_OMT_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence, detection of polymorphisms and radiation hybrid mapping of the equine catechol-O-methyltransferase gene."
      Momozawa Y., Takeuchi Y., Tozaki T., Kikusui T., Hasegawa T., Raudsepp T., Chowdhary B.P., Kusunose R., Mori Y.
      Anim. Genet. 36:190-190(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-120, ALTERNATIVE INITIATION.

    Entry informationi

    Entry nameiCOMT_HORSE
    AccessioniPrimary (citable) accession number: Q5H879
    Secondary accession number(s): Q5H878
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3