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Q5H879

- COMT_HORSE

UniProt

Q5H879 - COMT_HORSE

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Protein
Catechol O-methyltransferase
Gene
COMT
Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol By similarity.

Catalytic activityi

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei111 – 1111S-adenosyl-L-methionine By similarity
Binding sitei119 – 1191S-adenosyl-L-methionine By similarity
Binding sitei137 – 1371S-adenosyl-L-methionine By similarity
Binding sitei138 – 1381S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei166 – 1661S-adenosyl-L-methionine; via amide nitrogen By similarity
Metal bindingi188 – 1881Magnesium By similarity
Binding sitei188 – 1881S-adenosyl-L-methionine By similarity
Binding sitei191 – 1911Substrate By similarity
Metal bindingi216 – 2161Magnesium By similarity
Metal bindingi217 – 2171Magnesium By similarity
Binding sitei217 – 2171Substrate By similarity
Binding sitei246 – 2461Substrate By similarity

GO - Molecular functioni

  1. catechol O-methyltransferase activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro

GO - Biological processi

  1. catecholamine metabolic process Source: UniProtKB-KW
  2. neurotransmitter catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Catecholamine metabolism, Neurotransmitter degradation

Keywords - Ligandi

Magnesium, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Catechol O-methyltransferase (EC:2.1.1.6)
Gene namesi
Name:COMT
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281: Unplaced

Subcellular locationi

Isoform 2 : Cytoplasm By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei5 – 2521Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Catechol O-methyltransferase
PRO_0000318147Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5H879.
SMRiQ5H879. Positions 49-263.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni164 – 1674S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4122.
HOGENOMiHOG000046392.
HOVERGENiHBG005376.
InParanoidiQ5H879.
KOiK00545.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025782. Catechol_O-MeTrfase.
IPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10509. PTHR10509. 1 hit.
PfamiPF01596. Methyltransf_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51682. SAM_OMT_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform 1 (identifier: Q5H879-1) [UniParc]FASTAAdd to Basket

Also known as: Membrane-bound, MB-COMT

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLEPTPMLLA AFSLGLALLP LLFFLRRWGW LLIGWNECIL QPIHNLLMGD    50
SKEQRILRHV LQHAVAGDPQ SVLETIDAYC SQKEWAMNVG DKKGQFLDAV 100
VQEQQPSVLL ELGAYCGYSA VRMARLLPPG ARLLTIEINP DYAAITQRML 150
DFAGLQDRVT VVLGASQDII PQLKKKYDVD TLDVVFLDHW KDRYLPDTLL 200
LEECGLLRKG TVLLADNVIV PGAPDFLAHV RGSGRFECTH FSSYLEYSLW 250
VVDGVEKAVY LGPGSPAQP 269
Length:269
Mass (Da):30,028
Last modified:March 1, 2005 - v1
Checksum:i73A24585C6768C44
GO
Isoform 2 (identifier: Q5H879-2) [UniParc]FASTAAdd to Basket

Also known as: Soluble, S-COMT

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.

Show »
Length:222
Mass (Da):24,600
Checksum:i4E3097D0491A0103
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti120 – 1201A → T.1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4747Missing in isoform 2.
VSP_031176Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB178284 mRNA. Translation: BAD89294.1.
AB178284 mRNA. Translation: BAD89295.1.
RefSeqiNP_001075303.1. NM_001081834.1. [Q5H879-1]
NP_001108007.1. NM_001114535.1. [Q5H879-2]
UniGeneiEca.21379.

Genome annotation databases

GeneIDi100146509.
KEGGiecb:100146509.

Keywords - Coding sequence diversityi

Alternative initiation, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB178284 mRNA. Translation: BAD89294.1 .
AB178284 mRNA. Translation: BAD89295.1 .
RefSeqi NP_001075303.1. NM_001081834.1. [Q5H879-1 ]
NP_001108007.1. NM_001114535.1. [Q5H879-2 ]
UniGenei Eca.21379.

3D structure databases

ProteinModelPortali Q5H879.
SMRi Q5H879. Positions 49-263.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100146509.
KEGGi ecb:100146509.

Organism-specific databases

CTDi 1312.

Phylogenomic databases

eggNOGi COG4122.
HOGENOMi HOG000046392.
HOVERGENi HBG005376.
InParanoidi Q5H879.
KOi K00545.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR025782. Catechol_O-MeTrfase.
IPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10509. PTHR10509. 1 hit.
Pfami PF01596. Methyltransf_3. 1 hit.
[Graphical view ]
PIRSFi PIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51682. SAM_OMT_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence, detection of polymorphisms and radiation hybrid mapping of the equine catechol-O-methyltransferase gene."
    Momozawa Y., Takeuchi Y., Tozaki T., Kikusui T., Hasegawa T., Raudsepp T., Chowdhary B.P., Kusunose R., Mori Y.
    Anim. Genet. 36:190-190(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-120, ALTERNATIVE INITIATION.

Entry informationi

Entry nameiCOMT_HORSE
AccessioniPrimary (citable) accession number: Q5H879
Secondary accession number(s): Q5H878
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 1, 2005
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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