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Protein

Catechol O-methyltransferase

Gene

COMT

Organism
Equus caballus (Horse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei111 – 1111S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei119 – 1191S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei137 – 1371S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei138 – 1381S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei166 – 1661S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Metal bindingi188 – 1881MagnesiumBy similarity
Binding sitei188 – 1881S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei191 – 1911SubstrateBy similarity
Metal bindingi216 – 2161MagnesiumBy similarity
Metal bindingi217 – 2171MagnesiumBy similarity
Binding sitei217 – 2171SubstrateBy similarity
Binding sitei246 – 2461SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Catecholamine metabolism, Neurotransmitter degradation

Keywords - Ligandi

Magnesium, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Catechol O-methyltransferase (EC:2.1.1.6)
Gene namesi
Name:COMT
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281 Componenti: Unplaced

Subcellular locationi

Isoform 2 :

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44CytoplasmicSequence Analysis
Transmembranei5 – 2521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini26 – 269244ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Catechol O-methyltransferasePRO_0000318147Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi9796.ENSECAP00000005379.

Structurei

3D structure databases

ProteinModelPortaliQ5H879.
SMRiQ5H879. Positions 49-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni164 – 1674S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4122.
HOGENOMiHOG000046392.
HOVERGENiHBG005376.
InParanoidiQ5H879.
KOiK00545.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10509. PTHR10509. 1 hit.
PfamiPF01596. Methyltransf_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51682. SAM_OMT_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q5H879-1) [UniParc]FASTAAdd to basket

Also known as: Membrane-bound, MB-COMT

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLEPTPMLLA AFSLGLALLP LLFFLRRWGW LLIGWNECIL QPIHNLLMGD
60 70 80 90 100
SKEQRILRHV LQHAVAGDPQ SVLETIDAYC SQKEWAMNVG DKKGQFLDAV
110 120 130 140 150
VQEQQPSVLL ELGAYCGYSA VRMARLLPPG ARLLTIEINP DYAAITQRML
160 170 180 190 200
DFAGLQDRVT VVLGASQDII PQLKKKYDVD TLDVVFLDHW KDRYLPDTLL
210 220 230 240 250
LEECGLLRKG TVLLADNVIV PGAPDFLAHV RGSGRFECTH FSSYLEYSLW
260
VVDGVEKAVY LGPGSPAQP
Length:269
Mass (Da):30,028
Last modified:March 1, 2005 - v1
Checksum:i73A24585C6768C44
GO
Isoform 2 (identifier: Q5H879-2) [UniParc]FASTAAdd to basket

Also known as: Soluble, S-COMT

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.

Show »
Length:222
Mass (Da):24,600
Checksum:i4E3097D0491A0103
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti120 – 1201A → T.1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4747Missing in isoform 2. CuratedVSP_031176Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB178284 mRNA. Translation: BAD89294.1.
AB178284 mRNA. Translation: BAD89295.1.
RefSeqiNP_001075303.1. NM_001081834.1. [Q5H879-1]
NP_001108007.1. NM_001114535.1. [Q5H879-2]
UniGeneiEca.21379.

Genome annotation databases

GeneIDi100146509.
KEGGiecb:100146509.

Keywords - Coding sequence diversityi

Alternative initiation, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB178284 mRNA. Translation: BAD89294.1.
AB178284 mRNA. Translation: BAD89295.1.
RefSeqiNP_001075303.1. NM_001081834.1. [Q5H879-1]
NP_001108007.1. NM_001114535.1. [Q5H879-2]
UniGeneiEca.21379.

3D structure databases

ProteinModelPortaliQ5H879.
SMRiQ5H879. Positions 49-263.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9796.ENSECAP00000005379.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100146509.
KEGGiecb:100146509.

Organism-specific databases

CTDi1312.

Phylogenomic databases

eggNOGiCOG4122.
HOGENOMiHOG000046392.
HOVERGENiHBG005376.
InParanoidiQ5H879.
KOiK00545.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10509. PTHR10509. 1 hit.
PfamiPF01596. Methyltransf_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51682. SAM_OMT_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence, detection of polymorphisms and radiation hybrid mapping of the equine catechol-O-methyltransferase gene."
    Momozawa Y., Takeuchi Y., Tozaki T., Kikusui T., Hasegawa T., Raudsepp T., Chowdhary B.P., Kusunose R., Mori Y.
    Anim. Genet. 36:190-190(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-120, ALTERNATIVE INITIATION.

Entry informationi

Entry nameiCOMT_HORSE
AccessioniPrimary (citable) accession number: Q5H879
Secondary accession number(s): Q5H878
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 1, 2005
Last modified: June 24, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.