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Q5H879 (COMT_HORSE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Catechol O-methyltransferase

EC=2.1.1.6
Gene names
Name:COMT
OrganismEquus caballus (Horse) [Reference proteome]
Taxonomic identifier9796 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol By similarity.

Catalytic activity

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subcellular location

Isoform 2: Cytoplasm By similarity.

Isoform 1: Cell membrane; Single-pass type II membrane protein; Extracellular side By similarity.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: Q5H879-1)

Also known as: Membrane-bound; MB-COMT;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5H879-2)

Also known as: Soluble; S-COMT;

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269Catechol O-methyltransferase
PRO_0000318147

Regions

Transmembrane5 – 2521Helical; Signal-anchor for type II membrane protein; Potential
Region164 – 1674S-adenosyl-L-methionine binding By similarity

Sites

Metal binding1881Magnesium By similarity
Metal binding2161Magnesium By similarity
Metal binding2171Magnesium By similarity
Binding site891S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site1111S-adenosyl-L-methionine By similarity
Binding site1191S-adenosyl-L-methionine By similarity
Binding site1371S-adenosyl-L-methionine By similarity
Binding site1381S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site1661S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site1881S-adenosyl-L-methionine By similarity
Binding site1911Substrate By similarity
Binding site2171Substrate By similarity
Binding site2461Substrate By similarity

Natural variations

Alternative sequence1 – 4747Missing in isoform 2.
VSP_031176
Natural variant1201A → T. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Membrane-bound) (MB-COMT) [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: 73A24585C6768C44

FASTA26930,028
        10         20         30         40         50         60 
MLEPTPMLLA AFSLGLALLP LLFFLRRWGW LLIGWNECIL QPIHNLLMGD SKEQRILRHV 

        70         80         90        100        110        120 
LQHAVAGDPQ SVLETIDAYC SQKEWAMNVG DKKGQFLDAV VQEQQPSVLL ELGAYCGYSA 

       130        140        150        160        170        180 
VRMARLLPPG ARLLTIEINP DYAAITQRML DFAGLQDRVT VVLGASQDII PQLKKKYDVD 

       190        200        210        220        230        240 
TLDVVFLDHW KDRYLPDTLL LEECGLLRKG TVLLADNVIV PGAPDFLAHV RGSGRFECTH 

       250        260 
FSSYLEYSLW VVDGVEKAVY LGPGSPAQP 

« Hide

Isoform 2 (Soluble) (S-COMT) [UniParc].

Checksum: 4E3097D0491A0103
Show »

FASTA22224,600

References

[1]"Sequence, detection of polymorphisms and radiation hybrid mapping of the equine catechol-O-methyltransferase gene."
Momozawa Y., Takeuchi Y., Tozaki T., Kikusui T., Hasegawa T., Raudsepp T., Chowdhary B.P., Kusunose R., Mori Y.
Anim. Genet. 36:190-190(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-120, ALTERNATIVE INITIATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB178284 mRNA. Translation: BAD89294.1.
AB178284 mRNA. Translation: BAD89295.1.
RefSeqNP_001075303.1. NM_001081834.1.
NP_001108007.1. NM_001114535.1.
UniGeneEca.21379.

3D structure databases

ProteinModelPortalQ5H879.
SMRQ5H879. Positions 49-263.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100146509.
KEGGecb:100146509.

Organism-specific databases

CTD1312.

Phylogenomic databases

eggNOGCOG4122.
HOGENOMHOG000046392.
HOVERGENHBG005376.
InParanoidQ5H879.
KOK00545.

Family and domain databases

InterProIPR025782. Catechol_O-MeTrfase.
IPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
[Graphical view]
PANTHERPTHR10509. PTHR10509. 1 hit.
PfamPF01596. Methyltransf_3. 1 hit.
[Graphical view]
PIRSFPIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
PROSITEPS51682. SAM_OMT_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCOMT_HORSE
AccessionPrimary (citable) accession number: Q5H879
Secondary accession number(s): Q5H878
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 1, 2005
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families