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Protein

Catechol O-methyltransferase

Gene

COMT

Organism
Equus caballus (Horse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei111S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei119S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei137S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei138S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei166S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Metal bindingi188MagnesiumBy similarity1
Binding sitei188S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei191SubstrateBy similarity1
Metal bindingi216MagnesiumBy similarity1
Metal bindingi217MagnesiumBy similarity1
Binding sitei217SubstrateBy similarity1
Binding sitei246SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Catecholamine metabolism, Neurotransmitter degradation

Keywords - Ligandi

Magnesium, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Catechol O-methyltransferase (EC:2.1.1.6)
Gene namesi
Name:COMT
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
Proteomesi
  • UP000002281 Componenti: Unplaced

Subcellular locationi

Isoform 2 :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini26 – 269ExtracellularSequence analysisAdd BLAST244

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003181471 – 269Catechol O-methyltransferaseAdd BLAST269

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei265PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5H879.
PeptideAtlasiQ5H879.

Interactioni

Protein-protein interaction databases

STRINGi9796.ENSECAP00000005379.

Structurei

3D structure databases

ProteinModelPortaliQ5H879.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 167S-adenosyl-L-methionine bindingPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1663. Eukaryota.
COG4122. LUCA.
HOGENOMiHOG000046392.
HOVERGENiHBG005376.
InParanoidiQ5H879.
KOiK00545.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01596. Methyltransf_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51682. SAM_OMT_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q5H879-1) [UniParc]FASTAAdd to basket
Also known as: Membrane-bound, MB-COMT

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLEPTPMLLA AFSLGLALLP LLFFLRRWGW LLIGWNECIL QPIHNLLMGD
60 70 80 90 100
SKEQRILRHV LQHAVAGDPQ SVLETIDAYC SQKEWAMNVG DKKGQFLDAV
110 120 130 140 150
VQEQQPSVLL ELGAYCGYSA VRMARLLPPG ARLLTIEINP DYAAITQRML
160 170 180 190 200
DFAGLQDRVT VVLGASQDII PQLKKKYDVD TLDVVFLDHW KDRYLPDTLL
210 220 230 240 250
LEECGLLRKG TVLLADNVIV PGAPDFLAHV RGSGRFECTH FSSYLEYSLW
260
VVDGVEKAVY LGPGSPAQP
Length:269
Mass (Da):30,028
Last modified:March 1, 2005 - v1
Checksum:i73A24585C6768C44
GO
Isoform 2 (identifier: Q5H879-2) [UniParc]FASTAAdd to basket
Also known as: Soluble, S-COMT

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.

Show »
Length:222
Mass (Da):24,600
Checksum:i4E3097D0491A0103
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti120A → T.1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0311761 – 47Missing in isoform 2. CuratedAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB178284 mRNA. Translation: BAD89294.1.
AB178284 mRNA. Translation: BAD89295.1.
RefSeqiNP_001075303.1. NM_001081834.1. [Q5H879-1]
NP_001108007.1. NM_001114535.1. [Q5H879-2]
UniGeneiEca.21379.

Genome annotation databases

GeneIDi100146509.
KEGGiecb:100146509.

Keywords - Coding sequence diversityi

Alternative initiation, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB178284 mRNA. Translation: BAD89294.1.
AB178284 mRNA. Translation: BAD89295.1.
RefSeqiNP_001075303.1. NM_001081834.1. [Q5H879-1]
NP_001108007.1. NM_001114535.1. [Q5H879-2]
UniGeneiEca.21379.

3D structure databases

ProteinModelPortaliQ5H879.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9796.ENSECAP00000005379.

Proteomic databases

PaxDbiQ5H879.
PeptideAtlasiQ5H879.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100146509.
KEGGiecb:100146509.

Organism-specific databases

CTDi1312.

Phylogenomic databases

eggNOGiKOG1663. Eukaryota.
COG4122. LUCA.
HOGENOMiHOG000046392.
HOVERGENiHBG005376.
InParanoidiQ5H879.
KOiK00545.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01596. Methyltransf_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51682. SAM_OMT_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOMT_HORSE
AccessioniPrimary (citable) accession number: Q5H879
Secondary accession number(s): Q5H878
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 1, 2005
Last modified: October 5, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.