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Protein

Cell division cycle protein 20 homolog

Gene

CDC20

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-SSC-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-SSC-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-SSC-174154. APC/C:Cdc20 mediated degradation of Securin.
R-SSC-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-SSC-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-SSC-176407. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
R-SSC-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-SSC-176417. Phosphorylation of Emi1.
R-SSC-179409. APC-Cdc20 mediated degradation of Nek2A.
R-SSC-2467813. Separation of Sister Chromatids.
R-SSC-2500257. Resolution of Sister Chromatid Cohesion.
R-SSC-5663220. RHO GTPases Activate Formins.
R-SSC-68877. Mitotic Prometaphase.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle protein 20 homolog
Alternative name(s):
p55CDC
Gene namesi
Name:CDC20
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 6

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Cell division cycle protein 20 homologPRO_0000278194Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei41 – 411PhosphoserineBy similarity
Modified residuei66 – 661N6-acetyllysineBy similarity
Modified residuei70 – 701PhosphothreonineBy similarity
Modified residuei72 – 721PhosphoserineBy similarity
Modified residuei92 – 921PhosphoserineBy similarity
Modified residuei106 – 1061PhosphothreonineBy similarity
Modified residuei153 – 1531PhosphoserineBy similarity
Modified residuei157 – 1571PhosphothreonineBy similarity
Modified residuei161 – 1611PhosphoserineBy similarity
Cross-linki485 – 485Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki490 – 490Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161. Phosphorylated by NEK2 (By similarity).By similarity
Dephosphorylated by CTDP1.By similarity
Acetylated. Deacetylated at Lys-66 by SIRT2; deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C) (By similarity).By similarity
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5H7C0.

Expressioni

Gene expression databases

GenevisibleiQ5H7C0. SS.

Interactioni

Subunit structurei

Found in a complex with CDC20, CDC27, SIRT2, SPATC1 and TUBG1. Interacts with MAD2L1, BUB1B and SPATC1. The phosphorylated form interacts with APC/C. Interacts with NINL and MAD2L1. Interacts with CDK5RAP2. Interacts with NEUROD2 (By similarity). Interacts with NEK2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000004268.

Structurei

3D structure databases

ProteinModelPortaliQ5H7C0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati182 – 22140WD 1Add
BLAST
Repeati224 – 26340WD 2Add
BLAST
Repeati266 – 30338WD 3Add
BLAST
Repeati307 – 34640WD 4Add
BLAST
Repeati353 – 39543WD 5Add
BLAST
Repeati397 – 43842WD 6Add
BLAST
Repeati441 – 48040WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat CDC20/Fizzy family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0305. Eukaryota.
ENOG410XQ8I. LUCA.
GeneTreeiENSGT00840000129804.
HOGENOMiHOG000195514.
HOVERGENiHBG001024.
InParanoidiQ5H7C0.
KOiK03363.
OMAiQMEMANF.
OrthoDBiEOG091G06FJ.
TreeFamiTF101065.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR033187. CDC20.
IPR033010. Cdc20/Fizzy.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19918. PTHR19918. 1 hit.
PTHR19918:SF3. PTHR19918:SF3. 1 hit.
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5H7C0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQFVFESDL HSLLQLDAPI PNAPPARWQR KAKEAAGPAP SPMRAANRSH
60 70 80 90 100
SAGRTPGRTP GKSNSKMQTT PSKPGGDRYI PHRSASQMEV ASFLLSKENQ
110 120 130 140 150
PDNSQTPTKK EHQKAWALNL NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV
160 170 180 190 200
LYSQKATPGS SRKTCRYIPS LPDRILDAPE IRNDYYLNLV DWSSGNVLAV
210 220 230 240 250
ALDNSVYLWS ASSGDILQLL QMEQPGDYVS SVAWIKEGNY LAVGTSSAEV
260 270 280 290 300
QLWDVQQQKR LRNMTSHSAR VGSLCWNSYI LSSGSRSGHI HHHDVRVAEH
310 320 330 340 350
HVATLSGHSQ EVCGLRWAPD GRHLASGGND NLVNVWPSAP GEGGWVPLQT
360 370 380 390 400
FTQHQGAVKA VAWCPWQSNV LATGGGTSDR HIRIWNVCSG ACLSAVDAHS
410 420 430 440 450
QVCSILWSPH YKELISGHGF AQNQLVIWKY PTMAKVAELK GHTARVLSLT
460 470 480 490
MSPDGATVAS AAADETLRLW RCFELDPARR REREKASAAK SSLIHQGIR
Length:499
Mass (Da):54,784
Last modified:March 1, 2005 - v1
Checksum:iF07E9C7BC742799F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB192873 mRNA. Translation: BAD89276.1.
RefSeqiNP_001116566.1. NM_001123094.1.
UniGeneiSsc.17001.

Genome annotation databases

EnsembliENSSSCT00000004368; ENSSSCP00000004268; ENSSSCG00000003949.
GeneIDi397379.
KEGGissc:397379.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB192873 mRNA. Translation: BAD89276.1.
RefSeqiNP_001116566.1. NM_001123094.1.
UniGeneiSsc.17001.

3D structure databases

ProteinModelPortaliQ5H7C0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000004268.

Proteomic databases

PaxDbiQ5H7C0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000004368; ENSSSCP00000004268; ENSSSCG00000003949.
GeneIDi397379.
KEGGissc:397379.

Organism-specific databases

CTDi991.

Phylogenomic databases

eggNOGiKOG0305. Eukaryota.
ENOG410XQ8I. LUCA.
GeneTreeiENSGT00840000129804.
HOGENOMiHOG000195514.
HOVERGENiHBG001024.
InParanoidiQ5H7C0.
KOiK03363.
OMAiQMEMANF.
OrthoDBiEOG091G06FJ.
TreeFamiTF101065.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-SSC-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-SSC-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-SSC-174154. APC/C:Cdc20 mediated degradation of Securin.
R-SSC-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-SSC-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-SSC-176407. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
R-SSC-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-SSC-176417. Phosphorylation of Emi1.
R-SSC-179409. APC-Cdc20 mediated degradation of Nek2A.
R-SSC-2467813. Separation of Sister Chromatids.
R-SSC-2500257. Resolution of Sister Chromatid Cohesion.
R-SSC-5663220. RHO GTPases Activate Formins.
R-SSC-68877. Mitotic Prometaphase.

Gene expression databases

GenevisibleiQ5H7C0. SS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR033187. CDC20.
IPR033010. Cdc20/Fizzy.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19918. PTHR19918. 1 hit.
PTHR19918:SF3. PTHR19918:SF3. 1 hit.
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDC20_PIG
AccessioniPrimary (citable) accession number: Q5H7C0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: March 1, 2005
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.