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Q5H737 (TRMB_COLOR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Appressorial penetration into host protein 1
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:TRM8
Synonyms:APH1
ORF Names:Cob_06482
OrganismColletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422) (Cucumber anthracnose fungus) (Colletotrichum lagenarium) [Complete proteome]
Taxonomic identifier1213857 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeGlomerellalesGlomerellaceaemitosporic GlomerellaceaeColletotrichum

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with TRM82 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Disruption phenotype

Significant reduction in pathogenicity on the host plants. Mutants develop penetration hyphae into cellophane, suggesting that appressoria of the mutants retain basic function for penetration. However, they fail to develop intracellular penetration hyphae into epidermis of the host plants. Ref.1

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Sequence caution

The sequence ENH85272.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 286286tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370596

Regions

Region127 – 1282S-adenosyl-L-methionine binding By similarity
Region162 – 1632S-adenosyl-L-methionine binding By similarity
Region260 – 2623S-adenosyl-L-methionine binding By similarity

Sites

Active site1851 By similarity
Binding site1041S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1821S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5H737 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: A8FC0373CE5D9305

FASTA28632,714
        10         20         30         40         50         60 
MGRARPKSQK RGDYRVSRSQ ENAAELPKKK FYRQRAHANP FSDHHLVYPA CPDEMDWTLY 

        70         80         90        100        110        120 
YPAFPTQGET LPNHIFTHNQ SCMRISDSTR SGSLQKNIEV VDIGCGFGGL LVALAPLMPE 

       130        140        150        160        170        180 
TLALGLEIRT SVTEYVQEKI RALRAQNEGT GLYQNIGCIR ANSMKFLPNF LRKSQLSKIF 

       190        200        210        220        230        240 
ICFPDPHFKA RKHKARIVSA TLNSEYAFAL RPGGIVYTIT DVEPLHQWMA EHFVAHPSFE 

       250        260        270        280 
RLSQEEEEAD ECVQVMKSET EEGRKVTRNQ GQKFVALFRR IEDPPW 

« Hide

References

« Hide 'large scale' references
[1]"A gene involved in modifying transfer RNA is required for fungal pathogenicity and stress tolerance of Colletotrichum lagenarium."
Takano Y., Takayanagi N., Hori H., Ikeuchi Y., Suzuki T., Kimura A., Okuno T.
Mol. Microbiol. 60:81-92(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
Strain: 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422.
[2]"Comparative genomic and transcriptomic analyses reveal the hemibiotrophic stage shift of Colletotrichum fungi."
Gan P., Ikeda K., Irieda H., Narusaka M., O'Connell R.J., Narusaka Y., Takano Y., Kubo Y., Shirasu K.
New Phytol. 197:1236-1249(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB201305 Genomic DNA. Translation: BAD89293.1.
KB725789 Genomic DNA. Translation: ENH85272.1. Sequence problems.

3D structure databases

ProteinModelPortalQ5H737.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_COLOR
AccessionPrimary (citable) accession number: Q5H737
Secondary accession number(s): N4VN37
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2005
Last modified: June 11, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways