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Q5H737

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UniProt

Q5H737 - TRMB_COLOR

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Colletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422) (Cucumber anthracnose fungus) (Colletotrichum lagenarium)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei182 – 1821S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei185 – 1851UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    Appressorial penetration into host protein 1
    Transfer RNA methyltransferase 8UniRule annotation
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:TRM8UniRule annotation
    Synonyms:APH1
    ORF Names:Cob_06482
    OrganismiColletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422) (Cucumber anthracnose fungus) (Colletotrichum lagenarium)
    Taxonomic identifieri1213857 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeGlomerellalesGlomerellaceaemitosporic GlomerellaceaeColletotrichum
    ProteomesiUP000014480: Whole Genome Shotgun Assembly

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Significant reduction in pathogenicity on the host plants. Mutants develop penetration hyphae into cellophane, suggesting that appressoria of the mutants retain basic function for penetration. However, they fail to develop intracellular penetration hyphae into epidermis of the host plants.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 286286tRNA (guanine-N(7)-)-methyltransferasePRO_0000370596Add
    BLAST

    Interactioni

    Subunit structurei

    Forms a complex with TRM82.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ5H737.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni127 – 1282S-adenosyl-L-methionine bindingUniRule annotation
    Regioni162 – 1632S-adenosyl-L-methionine bindingUniRule annotation
    Regioni260 – 2623S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5H737-1 [UniParc]FASTAAdd to Basket

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    MGRARPKSQK RGDYRVSRSQ ENAAELPKKK FYRQRAHANP FSDHHLVYPA    50
    CPDEMDWTLY YPAFPTQGET LPNHIFTHNQ SCMRISDSTR SGSLQKNIEV 100
    VDIGCGFGGL LVALAPLMPE TLALGLEIRT SVTEYVQEKI RALRAQNEGT 150
    GLYQNIGCIR ANSMKFLPNF LRKSQLSKIF ICFPDPHFKA RKHKARIVSA 200
    TLNSEYAFAL RPGGIVYTIT DVEPLHQWMA EHFVAHPSFE RLSQEEEEAD 250
    ECVQVMKSET EEGRKVTRNQ GQKFVALFRR IEDPPW 286
    Length:286
    Mass (Da):32,714
    Last modified:March 1, 2005 - v1
    Checksum:iA8FC0373CE5D9305
    GO

    Sequence cautioni

    The sequence ENH85272.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB201305 Genomic DNA. Translation: BAD89293.1.
    KB725789 Genomic DNA. Translation: ENH85272.1. Sequence problems.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB201305 Genomic DNA. Translation: BAD89293.1 .
    KB725789 Genomic DNA. Translation: ENH85272.1 . Sequence problems.

    3D structure databases

    ProteinModelPortali Q5H737.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A gene involved in modifying transfer RNA is required for fungal pathogenicity and stress tolerance of Colletotrichum lagenarium."
      Takano Y., Takayanagi N., Hori H., Ikeuchi Y., Suzuki T., Kimura A., Okuno T.
      Mol. Microbiol. 60:81-92(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
      Strain: 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422.
    2. "Comparative genomic and transcriptomic analyses reveal the hemibiotrophic stage shift of Colletotrichum fungi."
      Gan P., Ikeda K., Irieda H., Narusaka M., O'Connell R.J., Narusaka Y., Takano Y., Kubo Y., Shirasu K.
      New Phytol. 197:1236-1249(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422.

    Entry informationi

    Entry nameiTRMB_COLOR
    AccessioniPrimary (citable) accession number: Q5H737
    Secondary accession number(s): N4VN37
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3