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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Colletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422) (Cucumber anthracnose fungus) (Colletotrichum lagenarium)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway:iN(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei182 – 1821S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei185 – 1851UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Appressorial penetration into host protein 1
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
Synonyms:APH1
ORF Names:Cob_06482
OrganismiColletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422) (Cucumber anthracnose fungus) (Colletotrichum lagenarium)
Taxonomic identifieri1213857 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeGlomerellalesGlomerellaceaeColletotrichum
ProteomesiUP000014480 Componenti: Whole Genome Shotgun Assembly

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Significant reduction in pathogenicity on the host plants. Mutants develop penetration hyphae into cellophane, suggesting that appressoria of the mutants retain basic function for penetration. However, they fail to develop intracellular penetration hyphae into epidermis of the host plants.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286tRNA (guanine-N(7)-)-methyltransferasePRO_0000370596Add
BLAST

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ5H737.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni127 – 1282S-adenosyl-L-methionine bindingUniRule annotation
Regioni162 – 1632S-adenosyl-L-methionine bindingUniRule annotation
Regioni260 – 2623S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5H737-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRARPKSQK RGDYRVSRSQ ENAAELPKKK FYRQRAHANP FSDHHLVYPA
60 70 80 90 100
CPDEMDWTLY YPAFPTQGET LPNHIFTHNQ SCMRISDSTR SGSLQKNIEV
110 120 130 140 150
VDIGCGFGGL LVALAPLMPE TLALGLEIRT SVTEYVQEKI RALRAQNEGT
160 170 180 190 200
GLYQNIGCIR ANSMKFLPNF LRKSQLSKIF ICFPDPHFKA RKHKARIVSA
210 220 230 240 250
TLNSEYAFAL RPGGIVYTIT DVEPLHQWMA EHFVAHPSFE RLSQEEEEAD
260 270 280
ECVQVMKSET EEGRKVTRNQ GQKFVALFRR IEDPPW
Length:286
Mass (Da):32,714
Last modified:March 1, 2005 - v1
Checksum:iA8FC0373CE5D9305
GO

Sequence cautioni

The sequence ENH85272.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB201305 Genomic DNA. Translation: BAD89293.1.
KB725789 Genomic DNA. Translation: ENH85272.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB201305 Genomic DNA. Translation: BAD89293.1.
KB725789 Genomic DNA. Translation: ENH85272.1. Sequence problems.

3D structure databases

ProteinModelPortaliQ5H737.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A gene involved in modifying transfer RNA is required for fungal pathogenicity and stress tolerance of Colletotrichum lagenarium."
    Takano Y., Takayanagi N., Hori H., Ikeuchi Y., Suzuki T., Kimura A., Okuno T.
    Mol. Microbiol. 60:81-92(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
    Strain: 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422.
  2. "Comparative genomic and transcriptomic analyses reveal the hemibiotrophic stage shift of Colletotrichum fungi."
    Gan P., Ikeda K., Irieda H., Narusaka M., O'Connell R.J., Narusaka Y., Takano Y., Kubo Y., Shirasu K.
    New Phytol. 197:1236-1249(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422.

Entry informationi

Entry nameiTRMB_COLOR
AccessioniPrimary (citable) accession number: Q5H737
Secondary accession number(s): N4VN37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2005
Last modified: March 4, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.