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Q5H737

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UniProt

Q5H737 - TRMB_COLOR

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene
TRM8, APH1, Cob_06482
Organism
Colletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422) (Cucumber anthracnose fungus) (Colletotrichum lagenarium)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei182 – 1821S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei185 – 1851 By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
Appressorial penetration into host protein 1
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:TRM8
Synonyms:APH1
ORF Names:Cob_06482
OrganismiColletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422) (Cucumber anthracnose fungus) (Colletotrichum lagenarium)
Taxonomic identifieri1213857 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeGlomerellalesGlomerellaceaemitosporic GlomerellaceaeColletotrichum
ProteomesiUP000014480: Whole Genome Shotgun Assembly

Subcellular locationi

Nucleus By similarity UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Significant reduction in pathogenicity on the host plants. Mutants develop penetration hyphae into cellophane, suggesting that appressoria of the mutants retain basic function for penetration. However, they fail to develop intracellular penetration hyphae into epidermis of the host plants.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286tRNA (guanine-N(7)-)-methyltransferaseUniRule annotationPRO_0000370596Add
BLAST

Interactioni

Subunit structurei

Forms a complex with TRM82 By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ5H737.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni127 – 1282S-adenosyl-L-methionine binding By similarity
Regioni162 – 1632S-adenosyl-L-methionine binding By similarity
Regioni260 – 2623S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5H737-1 [UniParc]FASTAAdd to Basket

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MGRARPKSQK RGDYRVSRSQ ENAAELPKKK FYRQRAHANP FSDHHLVYPA    50
CPDEMDWTLY YPAFPTQGET LPNHIFTHNQ SCMRISDSTR SGSLQKNIEV 100
VDIGCGFGGL LVALAPLMPE TLALGLEIRT SVTEYVQEKI RALRAQNEGT 150
GLYQNIGCIR ANSMKFLPNF LRKSQLSKIF ICFPDPHFKA RKHKARIVSA 200
TLNSEYAFAL RPGGIVYTIT DVEPLHQWMA EHFVAHPSFE RLSQEEEEAD 250
ECVQVMKSET EEGRKVTRNQ GQKFVALFRR IEDPPW 286
Length:286
Mass (Da):32,714
Last modified:March 1, 2005 - v1
Checksum:iA8FC0373CE5D9305
GO

Sequence cautioni

The sequence ENH85272.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB201305 Genomic DNA. Translation: BAD89293.1.
KB725789 Genomic DNA. Translation: ENH85272.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB201305 Genomic DNA. Translation: BAD89293.1 .
KB725789 Genomic DNA. Translation: ENH85272.1 . Sequence problems.

3D structure databases

ProteinModelPortali Q5H737.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A gene involved in modifying transfer RNA is required for fungal pathogenicity and stress tolerance of Colletotrichum lagenarium."
    Takano Y., Takayanagi N., Hori H., Ikeuchi Y., Suzuki T., Kimura A., Okuno T.
    Mol. Microbiol. 60:81-92(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
    Strain: 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422.
  2. "Comparative genomic and transcriptomic analyses reveal the hemibiotrophic stage shift of Colletotrichum fungi."
    Gan P., Ikeda K., Irieda H., Narusaka M., O'Connell R.J., Narusaka Y., Takano Y., Kubo Y., Shirasu K.
    New Phytol. 197:1236-1249(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422.

Entry informationi

Entry nameiTRMB_COLOR
AccessioniPrimary (citable) accession number: Q5H737
Secondary accession number(s): N4VN37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2005
Last modified: June 11, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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