Q5H6H2 (GLGB1_XANOR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB 1
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 1
Alpha-(1->4)-glucan branching enzyme 1
Glycogen branching enzyme 1
Short name=BE 1

Gene names

Name:	glgB1
Ordered Locus Names:	XOO0194

Organism

Xanthomonas oryzae pv. oryzae [Complete proteome] [HAMAP]

Taxonomic identifier

64187 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 732	732	1,4-alpha-glucan branching enzyme GlgB 1 HAMAP MF_00685		PRO_0000188768
Sites
	Active site	411	1	Nucleophile By similarity
	Active site	464	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5H6H2 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 5374208FE2C9B3F5	FASTA	732	80,923
	10 20 30 40 50 60 MMSGVMTAVS NRWDPGVIRA LAEARHGDAF AVLGAHRTDT GRVLRTYLPG AERVSAVLDD 70 80 90 100 110 120 GQTIALEAGP EPGLFAGDLP AQGGYRLRIG WPGGEQDTAD PYAFGPQLSD FDLHLISEGH 130 140 150 160 170 180 HLQLADALGA NVVEVDGVRG TRFAVWAPNA SRVAVVGDFN SWDARRHPMR LRHQSGVWEL 190 200 210 220 230 240 FVPDVGPGAH YKYQLRGPHG HELPAKADPV ARRAELAPGT ASIVADPTPY QWSDDGWMAT 250 260 270 280 290 300 RARRQAHDAP MSVYEMHAGS WLREEGVDLD WDGLADHLIP YVADMGFTHV ELMPVTEHPF 310 320 330 340 350 360 GGSWGYQPLG LFAPTARFGT PDGFARFVDR CHREGIGVIV DWVPAHFPTD AHGLAHFDGT 370 380 390 400 410 420 ALYEHADPRE GFHRDWNTLI YNHGRREVSG FLIASAMEFL QRYHVDGLRV DAVASMLYRD 430 440 450 460 470 480 YSRNAGEWVP NIHGGRENYE TIAFLRRLNA LVREHTPGAV MIAEESTAFP GVTADVAHGG 490 500 510 520 530 540 LGFHYKWNMG WMHDTLHYAS LDPIYRRYHH GELTFSMVYA YSERFVLPIS HDEVVHGKGS 550 560 570 580 590 600 LLGRMPGDDW QRFANLRAYL GFMFTHPGRK LLFMGCEFGQ PTEWNHDSGL PWHLLDDPRH 610 620 630 640 650 660 RGVQTLVRDV NRLYVQYPAL HAHDDDPSGF AWVVGDDAGN SVVAFLRKGK RGDAPVLVVI 670 680 690 700 710 720 NFTPVVQHGY RIGVPQGGQW REVFNSDAGI YGGANLGNGG IVTAEQQSMH GHAHALPLLL 730 PPLGAIVLTP PG

References

[1]

"The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the bacterial blight pathogen of rice." Lee B.-M., Park Y.-J., Park D.-S., Kang H.-W., Kim J.-G., Song E.-S., Park I.-C., Yoon U.-H., Hahn J.-H., Koo B.-S., Lee G.-B., Kim H., Park H.-S., Yoon K.-O., Kim J.-H., Jung C.-H., Koh N.-H., Seo J.-S., Go S.-J. Nucleic Acids Res. 33:577-586(2005) [PubMed: 15673718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KACC10331 / KXO85.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013598 Genomic DNA. Translation: AAW73448.1.
RefSeq	YP_198833.6. NC_006834.1.
3D structure databases
ProteinModelPortal	Q5H6H2.
SMR	Q5H6H2. Positions 120-731.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3264129.
GenomeReviews	Gene locus XOO0194 in contig AE013598_GR.
KEGG	xoo:XOO0194.
NMPDR	fig|291331.3.peg.567.
PATRIC	24099124. VBIXanOry111333_0226.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	RVYHQNG.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	XORY291331:XOO0194-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB1_XANOR

Accession

Primary (citable) accession number: Q5H6H2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program