Q5H2V2 (CHEB1_XANOR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 1
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	XOO1465

Organism

Xanthomonas oryzae pv. oryzae [Complete proteome] [HAMAP]

Taxonomic identifier

64187 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.
Sequence caution	The sequence AAW74719.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000225494
Regions
	Domain	1 – 115	115	Response regulatory
	Domain	161 – 351	191	CheB-type methylesterase
Sites
	Active site	173	1	By similarity
	Active site	199	1	By similarity
	Active site	295	1	By similarity
Amino acid modifications
	Modified residue	49	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5H2V2 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: B4DE2C22A9E04268	FASTA	351	37,278
	10 20 30 40 50 60 MVDDSAVVRQ VLVNVLNDAA DIEVIATAAD PLLAIEKMRK QWPDVIVLDV EMPRMDGITF 70 80 90 100 110 120 LRKIMSERPT PVVICSTLTE KGARVTMDAL AAGAVAVVTK PRLGLKQFLT ESADELVATV 130 140 150 160 170 180 RSAARANVKR LAARVTAVPL EAEVKHTADV ILPAQSGRAL AQTTERIVAI GTSTGGTQAL 190 200 210 220 230 240 EEVLTALPRV CPGIVIVQHM PEKFTAAFAA RLNGLCQIAV KEAANNDRVM PGRALIAPGG 250 260 270 280 290 300 KHLLLRRSGA QYFVEVLEGP PVNRHRPSVD VLFRSAARAA GSNALGIIMT GMGDDGAAGL 310 320 330 340 350 LEMRQAGART VAQDEQTSIV FGMPKEAIKR GGADRILPLG AMAREIVTQL Q

References

[1]

"The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the bacterial blight pathogen of rice." Lee B.-M., Park Y.-J., Park D.-S., Kang H.-W., Kim J.-G., Song E.-S., Park I.-C., Yoon U.-H., Hahn J.-H., Koo B.-S., Lee G.-B., Kim H., Park H.-S., Yoon K.-O., Kim J.-H., Jung C.-H., Koh N.-H., Seo J.-S., Go S.-J. Nucleic Acids Res. 33:577-586(2005) [PubMed: 15673718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KACC10331 / KXO85.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013598 Genomic DNA. Translation: AAW74719.1. Different initiation.
RefSeq	YP_200104.2. NC_006834.1.
3D structure databases
ProteinModelPortal	Q5H2V2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3262190.
GenomeReviews	Gene locus XOO1465 in contig AE013598_GR.
KEGG	xoo:XOO1465.
NMPDR	fig|291331.3.peg.3644.
PATRIC	24101980. VBIXanOry111333_1626.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG705324.
OMA	PGGKHML.
ProtClustDB	PRK00742.
Enzyme and pathway databases
BioCyc	XORY291331:XOO1465-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_XANOR

Accession

Primary (citable) accession number: Q5H2V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program