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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei240ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:XOO1504
OrganismiXanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85)
Taxonomic identifieri291331 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000006735 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197051 – 467Glutamate--tRNA ligaseAdd BLAST467

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

Secondary structure

1467
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Helixi17 – 32Combined sources16
Beta strandi36 – 41Combined sources6
Beta strandi46 – 49Combined sources4
Helixi51 – 64Combined sources14
Beta strandi69 – 74Combined sources6
Helixi75 – 78Combined sources4
Helixi79 – 91Combined sources13
Beta strandi94 – 98Combined sources5
Helixi102 – 113Combined sources12
Turni114 – 116Combined sources3
Beta strandi139 – 142Combined sources4
Beta strandi146 – 154Combined sources9
Turni155 – 157Combined sources3
Beta strandi158 – 164Combined sources7
Beta strandi171 – 173Combined sources3
Helixi181 – 191Combined sources11
Beta strandi196 – 200Combined sources5
Turni201 – 206Combined sources6
Helixi207 – 217Combined sources11
Beta strandi223 – 227Combined sources5
Beta strandi233 – 237Combined sources5
Beta strandi240 – 242Combined sources3
Helixi247 – 253Combined sources7
Helixi257 – 265Combined sources9
Turni266 – 268Combined sources3
Helixi279 – 285Combined sources7
Helixi300 – 313Combined sources14
Helixi316 – 329Combined sources14
Helixi340 – 347Combined sources8
Turni348 – 350Combined sources3
Helixi354 – 365Combined sources12
Helixi373 – 376Combined sources4
Helixi385 – 395Combined sources11
Helixi405 – 416Combined sources12
Helixi421 – 433Combined sources13
Beta strandi434 – 436Combined sources3
Helixi441 – 447Combined sources7
Helixi449 – 464Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5H4VX-ray3.00A/B/C/D/E/F1-467[»]
ProteinModelPortaliQ5H2R3.
SMRiQ5H2R3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionUniRule annotationAdd BLAST11
Motifi237 – 241"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

KOiK01885.
OMAiAYYAFDT.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5H2R3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACRTRFAPS PTGYLHIGGA RTALYCWLEA RRRGGQFVLR IEDTDRQRST
60 70 80 90 100
QAAIDAILEA MQWLGLGYDE GPIYQTQRVA RYQEVAEQLL AQGKAYYAYE
110 120 130 140 150
TREELDAMRE AAMAKQEKPR YDGAAREQNL PYRDDPNRVI RFKNPIGGTV
160 170 180 190 200
VFDDLIKGRI EIANSELDDM VIFRPDGLPT YNFAVVVDDW DMGITEVIRG
210 220 230 240 250
DDHINNTPRQ INIYAALGAP VPKFAHMPMI LDEQGTKLSK RTGAADVMQY
260 270 280 290 300
KDAGYLPHAL INYLARLGWS HGDQELFTPQ ELLDLFDVKD VNSKAARLDM
310 320 330 340 350
AKLGWVNQHY LKTDDPASIA PQLEYQLAKL GVDLAAGPAA ADVVVALRER
360 370 380 390 400
VHTLKEMAEK AVVWYQPLET YDAAAVMKHL KLGAEVPLGK ARELLAAVDQ
410 420 430 440 450
WSVDSVSAAL HDAAAALELG MGKVAQPLRV AITGTQVSPD ISQTVYLAGR
460
EGALKRIDAA LTKIGAA
Length:467
Mass (Da):51,649
Last modified:March 1, 2005 - v1
Checksum:i083DB8ACD9431ADF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013598 Genomic DNA. Translation: AAW74758.1.

Genome annotation databases

EnsemblBacteriaiAAW74758; AAW74758; XOO1504.
KEGGixoo:XOO1504.

Similar proteinsi

Entry informationi

Entry nameiSYE_XANOR
AccessioniPrimary (citable) accession number: Q5H2R3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 1, 2005
Last modified: January 31, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families