Reviewed, UniProtKB/Swiss-Prot Q5GYM5 (LEXA2_XANOR)

Names and origin

Protein names

Recommended name:
    LexA repressor 2
    EC=3.4.21.88

Gene names

Name:	lexA2
Ordered Locus Names:	XOO2942

Organism

Xanthomonas oryzae pv. oryzae [Complete proteome] [HAMAP]

Taxonomic identifier

64187 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity.
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	LexA repressor 2 HAMAP MF_00015		PRO_0000170112
Regions
	DNA binding	27 – 47	21	H-T-H motif By similarity
Sites
	Active site	133	1	For autocatalytic cleavage activity By similarity
	Active site	170	1	For autocatalytic cleavage activity By similarity
	Site	98 – 99	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5GYM5-1 [UniParc]. Last modified August 2, 2005. Version 2. Checksum: 1ACD7EAFE45D0ECC	FASTA	213	23,173
	10 20 30 40 50 60 MDLTDTQQAI LALIAERIDA DGVPPSQTEI ARAFGFKGVR AAQYHLEALE QAGAIRRVPG 70 80 90 100 110 120 QARGIRLAGQ GGQTRAAAVS EPVRDDVLRL PVLGRVAAGL PIGADIGSDD FVVLDRVFFS 130 140 150 160 170 180 PSPDYLLKVQ GDSMRDEGIF NGDLIGVHRT RDARSGQIVV ARIDEEITVK LLKIGKDRIR 190 200 210 LLPRNPDYAP IEVLPDQDFA IEGLYCGLLR PNR

References

[1]

"The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the bacterial blight pathogen of rice." Lee B.-M., Park Y.-J., Park D.-S., Kang H.-W., Kim J.-G., Song E.-S., Park I.-C., Yoon U.-H., Hahn J.-H., Koo B.-S., Lee G.-B., Kim H., Park H.-S., Yoon K.-O., Kim J.-H., Jung C.-H., Koh N.-H., Seo J.-S., Go S.-J. Nucleic Acids Res. 33:577-586(2005) [PubMed: 15673718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KACC10331 / KXO85.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013598 Genomic DNA. Translation: AAW76196.1. Different initiation.
RefSeq	YP_201581.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3264355.
GenomeReviews	Gene locus XOO2942 in contig AE013598_GR.
KEGG	xoo:XOO2942.
NMPDR	fig|291331.3.peg.4584.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5GYM5.
OMA	ITNHINA.
Enzyme and pathway databases
BioCyc	XORY291331:XOO2942-MON.
Family and domain databases
HAMAP	MF_00015. [Tree]
InterPro	IPR006199. LexA_DNA_bd. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA2_XANOR

Accession

Primary (citable) accession number: Q5GYM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2005
Last sequence update:	August 2, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)