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Q5GXV3

- PNP_XANOR

UniProt

Q5GXV3 - PNP_XANOR

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, XOO3214
Organism
Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi487 – 4871Magnesium By similarity
Metal bindingi493 – 4931Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciXORY291331:GJBV-2986-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:XOO3214
OrganismiXanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85)
Taxonomic identifieri291331 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000006735: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 704704Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000329942Add
BLAST

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Protein-protein interaction databases

STRINGi291331.XOO3214.

Structurei

3D structure databases

ProteinModelPortaliQ5GXV3.
SMRiQ5GXV3. Positions 618-691.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini554 – 61360KH
Add
BLAST
Domaini623 – 69169S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5GXV3-1 [UniParc]FASTAAdd to Basket

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MAKITKTFQY GKHTVTLETG EIARQAGGAV IVKFDDTVLL VTAVAAKSAR    50
EGQDFFPLTV DYQEKFYAGG RIPGGFFKRE GRATEKETLI SRLIDRPIRP 100
LFPEDYKNEV QIIATVMSMN PDIDGDIAAL IGASAALSLA GTPFNGPIAA 150
AKVGYKNGEY ILNPTVTDLK DSQLELVVAG TANAVLMVES EAELLSEEVM 200
LGAVTFGHRE MQKVINIINE LAVEAGTKPS DWVAPAKNDG MIAALKEAVG 250
DQLASAFQVR DKLQRRDAIS AIKKDVLGAL APRATIEGWA AGDLAKEFGE 300
LEYQTMRGSV LSTKVRIDGR ALDTVRPISA KAGVLPRTHG SALFTRGETQ 350
AIVITTLGTA RDGQVIDAVS GEYKENFLFH YNFPPYSVGE CGRFGAPKRR 400
EIGHGRLAKR GVLAVMPSLE EFPYTIRVVS EITESNGSSS MASVCGSSLA 450
LMDAGVPIKA PVAGIAMGLV KEGNDFVVLS DILGDEDHLG DMDFKVAGTA 500
EGVSALQMDI KIEGITEEIM KQALQQAKAG RLHILGEMAH ALTTPRQELS 550
DYAPRLLTIK IHPDKIREVI GKGGSTIQAI TKETGTQIDI QDDGTIIIAS 600
VNAIAAQAAK SRIEQITSDV EPGRIYEGKV AKIMDFGAFV TILPGKDGLV 650
HVSQISSERV EKVGDKLKEG DLVRVKVLEV DKQGRIRLSI KAVEEGEGVP 700
ASAE 704
Length:704
Mass (Da):75,434
Last modified:March 1, 2005 - v1
Checksum:i68E069C843CF0F13
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013598 Genomic DNA. Translation: AAW76468.1.
RefSeqiYP_201853.1. NC_006834.1.

Genome annotation databases

EnsemblBacteriaiAAW76468; AAW76468; XOO3214.
GeneIDi3265163.
KEGGixoo:XOO3214.
PATRICi24105886. VBIXanOry111333_3562.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013598 Genomic DNA. Translation: AAW76468.1 .
RefSeqi YP_201853.1. NC_006834.1.

3D structure databases

ProteinModelPortali Q5GXV3.
SMRi Q5GXV3. Positions 618-691.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 291331.XOO3214.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAW76468 ; AAW76468 ; XOO3214 .
GeneIDi 3265163.
KEGGi xoo:XOO3214.
PATRICi 24105886. VBIXanOry111333_3562.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci XORY291331:GJBV-2986-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KACC10331 / KXO85.

Entry informationi

Entry nameiPNP_XANOR
AccessioniPrimary (citable) accession number: Q5GXV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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