Q5GWB5 (TYSY_XANOR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	XOO3752

Organism

Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85) [Complete proteome] [HAMAP]

Taxonomic identifier

291331 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas › Xanthomonas oryzae › Xanthomonas oryzae pv. oryzae

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00008
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP-Rule MF_00008		PRO_0000141047
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5GWB5 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: F7850B09C1E34FD5	FASTA	264	30,105
	10 20 30 40 50 60 MKPYLDLLQH VLEHGAEKSD RTGTGTRSVF GWQMRFDLND GFPLVTTKKL HLRSIIHELL 70 80 90 100 110 120 WFLQGDTNIG YLSDNQVRIW DEWADDNGDL GPVYGKQWRR WTGPDGVEID QMQWLVDEIK 130 140 150 160 170 180 RNPDSRRLVI SAWNVGELPQ MALMPCHSLF QFYVVNGKLS CQLYQRSGDI FLGVPFNIAS 190 200 210 220 230 240 YALLTHMVAQ ATGLGVGDFV HTLGDAHLYS NHFDQARKQL TRTPRALPTL RLNPEVTDLF 250 260 AFRFEDIAIE GYDPHPAIKA PVAV

References

[1]

"The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the bacterial blight pathogen of rice." Lee B.-M., Park Y.-J., Park D.-S., Kang H.-W., Kim J.-G., Song E.-S., Park I.-C., Yoon U.-H., Hahn J.-H., Koo B.-S., Lee G.-B., Kim H., Park H.-S., Yoon K.-O., Kim J.-H., Jung C.-H., Koh N.-H., Seo J.-S., Go S.-J. Nucleic Acids Res. 33:577-586(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KACC10331 / KXO85.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013598 Genomic DNA. Translation: AAW77006.1.
RefSeq	YP_202391.1. NC_006834.1.
3D structure databases
ProteinModelPortal	Q5GWB5.
SMR	Q5GWB5. Positions 1-264.
ModBase	Search...
Protein-protein interaction databases
STRING	291331.XOO3752.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW77006; AAW77006; XOO3752.
GeneID	3264793.
KEGG	xoo:XOO3752.
PATRIC	24107086. VBIXanOry111333_4156.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
KO	K00560.
OMA	DTNVAYL.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	XORY291331:GJBV-3487-MONOMER.
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 1 hit.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_XANOR

Accession

Primary (citable) accession number: Q5GWB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	March 1, 2005
Last modified:	May 29, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program