Q5GTB0 (ISPE_WOLTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Wbm0173

Organism

Wolbachia sp. subsp. Brugia malayi (strain TRS) [Complete proteome] [HAMAP]

Taxonomic identifier

292805 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Wolbachieae › Wolbachia

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235152
Regions
	Nucleotide binding	100 – 110	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	140	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5GTB0 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 9F56957C4BE7863B	FASTA	288	32,007
	10 20 30 40 50 60 MKSFCVKAPA KINLFLHVVE KKETGYHLIE GLFVFANLSN FLEIKVGEKD FRYDNPIVEF 70 80 90 100 110 120 VNSELKISNK YNTVMRAVNL LLRHAPVRTK VTVKVVKNIP TSAGLGSGSS DAGAVIRTLG 130 140 150 160 170 180 KLWKIDRPIL NEIALSVGAD VPASIDSKPV LVRGIGEELC YINKFSLPTN IVLAKPKKKF 190 200 210 220 230 240 LSTPEVFSKY GGNFSKPIEW RDDTEKDLLK LLKETENDLQ EIAISLVPEI RDVILALESQ 250 260 270 280 EGSILSRMSG SGVTCFGIFD SEENAKTAAV NIREKQPEWW VCDAQLIV

References

[1]

"The Wolbachia genome of Brugia malayi: endosymbiont evolution within a human pathogenic nematode." Foster J., Ganatra M., Kamal I., Ware J., Makarova K., Ivanova N., Bhattacharyya A., Kapatral V., Kumar S., Posfai J., Vincze T., Ingram J., Moran L., Lapidus A., Omelchenko M., Kyrpides N., Ghedin E., Wang S. , Goltsman E., Joukov V., Ostrovskaya O., Tsukerman K., Mazur M., Comb D., Koonin E., Slatko B. PLoS Biol. 3:599-614(2005) [PubMed: 15780005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TRS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017321 Genomic DNA. Translation: AAW70764.1.
RefSeq	YP_198006.1. NC_006833.1.
3D structure databases
ProteinModelPortal	Q5GTB0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5GTB0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBWOLT00000003215; EBWOLP00000002910; EBWOLG00000003215.
GeneID	3267021.
GenomeReviews	Gene locus Wbm0173 in contig AE017321_GR.
KEGG	wbm:Wbm0173.
PATRIC	24031809. VBIWolEnd7741_0286.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
GeneTree	EBGT00050000030880.
HOGENOM	HBG734593.
OMA	QLCFGRG.
PhylomeDB	Q5GTB0.
ProtClustDB	PRK14610.
Enzyme and pathway databases
BioCyc	WPIP80849:WBM0173-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_WOLTR

Accession

Primary (citable) accession number: Q5GTB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program