Q5GT41 (MDH_WOLTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	Wbm0244

Organism

Wolbachia sp. subsp. Brugia malayi (strain TRS) [Complete proteome] [HAMAP]

Taxonomic identifier

292805 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Wolbachieae › Wolbachia › Wolbachia endosymbiont of Brugia malayi

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_0000113478
Regions
	Nucleotide binding	12 – 17	6	NAD By similarity
	Nucleotide binding	121 – 123	3	NAD By similarity
Sites
	Active site	178	1	Proton acceptor By similarity
	Binding site	36	1	NAD By similarity
	Binding site	85	1	Substrate By similarity
	Binding site	91	1	Substrate By similarity
	Binding site	98	1	NAD By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	154	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5GT41 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 759FBC8C6189EF32	FASTA	316	34,224
	10 20 30 40 50 60 MTVQRKKISL IGAGNIGGAL AHMVTLRELG DVVLFDVNDG IPQGKALDIA ESSPIGGFSV 70 80 90 100 110 120 NIIGTNRYGD IKNSDAIIIT AGIARKPGMS RDDLLQTNAK VMKEVGENIR KYSPNAFVIV 130 140 150 160 170 180 VTNPLDAMVS VVHKFSNLPA NMIVGMAGVL DSSRFRYFLA RELNISVEDV SAFVLGGHGD 190 200 210 220 230 240 TMVPLIRCAS IAGIPLTQII DMGLITQEKV DEIVKRTRNG GKEIIDLLKS GSAYYAPASS 250 260 270 280 290 300 SIYMLESYLR DEKRILPCAT YLNGEYGVKD LFIGVPVIIG KNGIEKVLEV KMDDSEQEMF 310 NKSVNAVKEL VKSLSL

References

[1]

"The Wolbachia genome of Brugia malayi: endosymbiont evolution within a human pathogenic nematode." Foster J., Ganatra M., Kamal I., Ware J., Makarova K., Ivanova N., Bhattacharyya A., Kapatral V., Kumar S., Posfai J., Vincze T., Ingram J., Moran L., Lapidus A., Omelchenko M., Kyrpides N., Ghedin E., Wang S. , Goltsman E., Joukov V., Ostrovskaya O., Tsukerman K., Mazur M., Comb D., Koonin E., Slatko B. PLoS Biol. 3:599-614(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TRS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017321 Genomic DNA. Translation: AAW70833.1.
RefSeq	YP_198075.1. NC_006833.1.
3D structure databases
ProteinModelPortal	Q5GT41.
SMR	Q5GT41. Positions 5-314.
ModBase	Search...
Protein-protein interaction databases
STRING	292805.Wbm0244.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW70833; AAW70833; Wbm0244.
GeneID	3266757.
KEGG	wbm:Wbm0244.
PATRIC	24032031. VBIWolEnd7741_0395.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213794.
KO	K00024.
OMA	DAMTYVM.
ProtClustDB	PRK06223.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_WOLTR

Accession

Primary (citable) accession number: Q5GT41

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	March 1, 2005
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program