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Q5GSS3

- SYI_WOLTR

UniProt

Q5GSS3 - SYI_WOLTR

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Wolbachia sp. subsp. Brugia malayi (strain TRS)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei659 – 6591ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciWEND292805:GH18-409-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Wbm0363
    OrganismiWolbachia sp. subsp. Brugia malayi (strain TRS)
    Taxonomic identifieri292805 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia
    ProteomesiUP000000534: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11341134Isoleucine--tRNA ligasePRO_0000098573Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi292805.Wbm0363.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5GSS3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi52 – 6211"HIGH" regionAdd
    BLAST
    Motifi656 – 6605"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiYETLYEC.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 3 hits.
    SSF50677. SSF50677. 2 hits.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5GSS3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPKHYPDTI SSPDFSSLEK EIIKFWQENK IFEQSVEKRS KDNCFVFYDG     50
    PPFANGLPHY GHLLTGFIKD AFARYQTMLQ KKVERRFGWD CHGLPAEMGA 100
    EKELGISGRT EIEKFGIEKF NNYCRTSVMK FSSEWEKYVN RQARWVDFHN 150
    DYKTMDRSFM ESVIWAFKQL YDKGLVYESV RVVPYSWACE TPLSNFETRL 200
    DTAYRKKTSK AVTVAFELLE NPQQFKQKCK LLAWTTTPWT LPSNLALAIG 250
    KDIEYCAVSV HSSVSFQHVT LESREKETWI PLSRTGMTEE GTETVTEDNE 300
    VSLVNNEIYI FAEDYLEKFI GHCEQNNIPY ENCNTKLKAD DLAGLSYKPL 350
    FNYFKGTKNA FRVFIADYVT GEDGTGVVHT APGFGEEDFY LCQSHDIPAI 400
    CPIDNSGRFT AEVSDLTGIH VFDTNDAIIK KLKEQGNWFK TEQYIHNYPH 450
    CWRTDTPLIY RAMPSWYVAV TKFKERMIEL NKRVNWIPTH IRDGQFGKWL 500
    ERAHDWSISR NRFWGTPIPI WKSDNARYPR VDVYGSIAEL ERDFNVKVND 550
    LHRPFIDSLT RPNPDDPTGK SIMRRVPDVF DCWFESGSVP FAQVHYPFEN 600
    KEWFESNFPA DFITEYIAQT RGWFYTLFVL STALFDSEPF KNCICHGVVL 650
    DVKGQKLSKR LNNYADPMEV FDKYGSDALR FLMLSGSIVC GGNLFLNKEG 700
    SSIRDVLKNV MKPIWNSYHF FTMYANADGI KAEVCKDYKS TIDSYMIFKC 750
    FEAVESIQAS MSNYNSQEAC KILIDFFEVL NNWYIRRSRE RFWKSNLDQD 800
    KTDAYNVLYT VFYYILRAAV PLLPLITETI WQGLKYKEIS VHLADFPQLE 850
    RYDSELIAKM DLVREICNSA LSIRNTFNIR VRQPLGSMTV YHQSSCSFLE 900
    SKPLSVVIPK FSPVIPMRDT GTQKEKKWSN AEMKTSMNEY QEMIKDEVNV 950
    KELKLVNSLE GIASLELKLN FPMLGKRIPD KVKKLVQYVK EGKWKQVDNE 1000
    QVFLGNESES YIIEKDEYEL LLKTNSEYSS VFGDNKGIVI LNTALDDALV 1050
    LEGLARDVVR LIQEARKQAD FHISDRIRVI IKTEDEKIKR AINTWGEYIR 1100
    EQTLSLSLEI NIEIGDNFYS KEYQDLIVGI ELNC 1134
    Length:1,134
    Mass (Da):131,811
    Last modified:March 1, 2005 - v1
    Checksum:iA3EDE2284B90E9B1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017321 Genomic DNA. Translation: AAW70951.1.
    RefSeqiYP_198193.1. NC_006833.1.

    Genome annotation databases

    EnsemblBacteriaiAAW70951; AAW70951; Wbm0363.
    GeneIDi3266894.
    KEGGiwbm:Wbm0363.
    PATRICi24032379. VBIWolEnd7741_0567.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017321 Genomic DNA. Translation: AAW70951.1 .
    RefSeqi YP_198193.1. NC_006833.1.

    3D structure databases

    ProteinModelPortali Q5GSS3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 292805.Wbm0363.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAW70951 ; AAW70951 ; Wbm0363 .
    GeneIDi 3266894.
    KEGGi wbm:Wbm0363.
    PATRICi 24032379. VBIWolEnd7741_0567.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi YETLYEC.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci WEND292805:GH18-409-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 3 hits.
    SSF50677. SSF50677. 2 hits.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TRS.

    Entry informationi

    Entry nameiSYI_WOLTR
    AccessioniPrimary (citable) accession number: Q5GSS3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3