SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5GSS3

- SYI_WOLTR

UniProt

Q5GSS3 - SYI_WOLTR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, Wbm0363
Organism
Wolbachia sp. subsp. Brugia malayi (strain TRS)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei659 – 6591ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciWEND292805:GH18-409-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Wbm0363
OrganismiWolbachia sp. subsp. Brugia malayi (strain TRS)
Taxonomic identifieri292805 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia
ProteomesiUP000000534: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11341134Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098573Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi292805.Wbm0363.

Structurei

3D structure databases

ProteinModelPortaliQ5GSS3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi52 – 6211"HIGH" regionUniRule annotation
Add
BLAST
Motifi656 – 6605"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiYETLYEC.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 3 hits.
SSF50677. SSF50677. 2 hits.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5GSS3-1 [UniParc]FASTAAdd to Basket

« Hide

MKPKHYPDTI SSPDFSSLEK EIIKFWQENK IFEQSVEKRS KDNCFVFYDG     50
PPFANGLPHY GHLLTGFIKD AFARYQTMLQ KKVERRFGWD CHGLPAEMGA 100
EKELGISGRT EIEKFGIEKF NNYCRTSVMK FSSEWEKYVN RQARWVDFHN 150
DYKTMDRSFM ESVIWAFKQL YDKGLVYESV RVVPYSWACE TPLSNFETRL 200
DTAYRKKTSK AVTVAFELLE NPQQFKQKCK LLAWTTTPWT LPSNLALAIG 250
KDIEYCAVSV HSSVSFQHVT LESREKETWI PLSRTGMTEE GTETVTEDNE 300
VSLVNNEIYI FAEDYLEKFI GHCEQNNIPY ENCNTKLKAD DLAGLSYKPL 350
FNYFKGTKNA FRVFIADYVT GEDGTGVVHT APGFGEEDFY LCQSHDIPAI 400
CPIDNSGRFT AEVSDLTGIH VFDTNDAIIK KLKEQGNWFK TEQYIHNYPH 450
CWRTDTPLIY RAMPSWYVAV TKFKERMIEL NKRVNWIPTH IRDGQFGKWL 500
ERAHDWSISR NRFWGTPIPI WKSDNARYPR VDVYGSIAEL ERDFNVKVND 550
LHRPFIDSLT RPNPDDPTGK SIMRRVPDVF DCWFESGSVP FAQVHYPFEN 600
KEWFESNFPA DFITEYIAQT RGWFYTLFVL STALFDSEPF KNCICHGVVL 650
DVKGQKLSKR LNNYADPMEV FDKYGSDALR FLMLSGSIVC GGNLFLNKEG 700
SSIRDVLKNV MKPIWNSYHF FTMYANADGI KAEVCKDYKS TIDSYMIFKC 750
FEAVESIQAS MSNYNSQEAC KILIDFFEVL NNWYIRRSRE RFWKSNLDQD 800
KTDAYNVLYT VFYYILRAAV PLLPLITETI WQGLKYKEIS VHLADFPQLE 850
RYDSELIAKM DLVREICNSA LSIRNTFNIR VRQPLGSMTV YHQSSCSFLE 900
SKPLSVVIPK FSPVIPMRDT GTQKEKKWSN AEMKTSMNEY QEMIKDEVNV 950
KELKLVNSLE GIASLELKLN FPMLGKRIPD KVKKLVQYVK EGKWKQVDNE 1000
QVFLGNESES YIIEKDEYEL LLKTNSEYSS VFGDNKGIVI LNTALDDALV 1050
LEGLARDVVR LIQEARKQAD FHISDRIRVI IKTEDEKIKR AINTWGEYIR 1100
EQTLSLSLEI NIEIGDNFYS KEYQDLIVGI ELNC 1134
Length:1,134
Mass (Da):131,811
Last modified:March 1, 2005 - v1
Checksum:iA3EDE2284B90E9B1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017321 Genomic DNA. Translation: AAW70951.1.
RefSeqiYP_198193.1. NC_006833.1.

Genome annotation databases

EnsemblBacteriaiAAW70951; AAW70951; Wbm0363.
GeneIDi3266894.
KEGGiwbm:Wbm0363.
PATRICi24032379. VBIWolEnd7741_0567.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017321 Genomic DNA. Translation: AAW70951.1 .
RefSeqi YP_198193.1. NC_006833.1.

3D structure databases

ProteinModelPortali Q5GSS3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 292805.Wbm0363.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAW70951 ; AAW70951 ; Wbm0363 .
GeneIDi 3266894.
KEGGi wbm:Wbm0363.
PATRICi 24032379. VBIWolEnd7741_0567.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi YETLYEC.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci WEND292805:GH18-409-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 3 hits.
SSF50677. SSF50677. 2 hits.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TRS.

Entry informationi

Entry nameiSYI_WOLTR
AccessioniPrimary (citable) accession number: Q5GSS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi