Q5GSK8 (Q5GSK8_WOLTR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A HAMAP-Rule MF_00171
EC=5.4.99.12 HAMAP-Rule MF_00171

Alternative name(s):
tRNA pseudouridine(38-40) synthase HAMAP-Rule MF_00171
tRNA pseudouridylate synthase I HAMAP-Rule MF_00171
tRNA-uridine isomerase I HAMAP-Rule MF_00171

Gene names

Name:	truA HAMAP-Rule MF_00171
Ordered Locus Names:	Wbm0428

Organism

Wolbachia sp. subsp. Brugia malayi (strain TRS) [Complete proteome] [HAMAP] EMBL AAW71016.1

Taxonomic identifier

292805 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Wolbachieae › Wolbachia › Wolbachia endosymbiont of Brugia malayi

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171 SAAS SAAS020094
Catalytic activity	tRNA uridine = tRNA pseudouridine. RuleBase RU003792 tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171 SAAS SAAS020094
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family. HAMAP-Rule MF_00171 RuleBase RU003792

Ontologies

Keywords
Biological process	tRNA processing HAMAP-Rule MF_00171 SAAS SAAS020094 RuleBase RU003792
Molecular function	Isomerase HAMAP-Rule MF_00171 SAAS SAAS020094 RuleBase RU003792
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	56	1	Nucleophile By similarity HAMAP-Rule MF_00171
	Binding site	115	1	Substrate By similarity HAMAP-Rule MF_00171

Sequences

	Sequence		Length	Mass (Da)
	Q5GSK8 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 638086EB69435627	FASTA	249	28,367
	10 20 30 40 50 60 MDFAVRYKIT IEYNGSSFSG WQKQQHSANS IQETIENAIF NFSGEKVTLH CGGRTDAGVH 70 80 90 100 110 120 ALGQVAHFDM ERKFELYKIR NAINYHLKSI PIVVLNAETV DNTFHSRFSA KKRYYEYRII 130 140 150 160 170 180 NRYAPAALEA GYAWQVFNPL DVNIMREAAK YLLGKHNLLS FRSKDCQATN PIRTIDDIDI 190 200 210 220 230 240 VQNGSHIYIK ISAISFLHNQ VRIIVGTLVE FGKSRTNPQK MLNILNQCKR NAAGVTAPPF GLYLAKIDY

References

[1]

"The Wolbachia genome of Brugia malayi: endosymbiont evolution within a human pathogenic nematode." Foster J., Ganatra M., Kamal I., Ware J., Makarova K., Ivanova N., Bhattacharyya A., Kapatral V., Kumar S., Posfai J., Vincze T., Ingram J., Moran L., Lapidus A., Omelchenko M., Kyrpides N., Ghedin E., Wang S. , Goltsman E., Joukov V., Ostrovskaya O., Tsukerman K., Mazur M., Comb D., Koonin E., Slatko B. PLoS Biol. 3:599-614(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TRS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017321 Genomic DNA. Translation: AAW71016.1.
RefSeq	YP_198258.1. NC_006833.1.
3D structure databases
ProteinModelPortal	Q5GSK8.
ModBase	Search...
Protein-protein interaction databases
STRING	292805.Wbm0428.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW71016; AAW71016; Wbm0428.
GeneID	3266792.
KEGG	wbm:Wbm0428.
PATRIC	24032599. VBIWolEnd7741_0673.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0101.
HOGENOM	HOG000248672.
KO	K06173.
OMA	RHYRYRI.
ProtClustDB	PRK00021.
Family and domain databases
Gene3D	3.30.70.580. 1 hit. 3.30.70.660. 1 hit.
HAMAP	MF_00171. TruA.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
PANTHER	PTHR11142. PTHR11142. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q5GSK8_WOLTR

Accession

Primary (citable) accession number: Q5GSK8

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)