Q5GSI7 (DAPD_WOLTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
EC=2.3.1.117

Alternative name(s):
Tetrahydrodipicolinate N-succinyltransferase
Short name=THDP succinyltransferase
Short name=THP succinyltransferase
Short name=Tetrahydropicolinate succinylase

Gene names

Name:	dapD
Ordered Locus Names:	Wbm0449

Organism

Wolbachia sp. subsp. Brugia malayi (strain TRS) [Complete proteome] [HAMAP]

Taxonomic identifier

292805 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Wolbachieae › Wolbachia › Wolbachia endosymbiont of Brugia malayi

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Succinyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H2O = CoA + N-succinyl-L-2-amino-6-oxoheptanedioate. HAMAP-Rule MF_00811
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 1/3. HAMAP-Rule MF_00811
Subunit structure	Homotrimer By similarity. HAMAP-Rule MF_00811
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00811.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.
Sequence caution	The sequence AAW71037.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP lysine biosynthetic process via diaminopimelate Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase HAMAP-Rule MF_00811		PRO_0000196975
Sites
	Binding site	106	1	Substrate By similarity
	Binding site	143	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5GSI7 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 42A8C3264C67CD55	FASTA	273	30,284
	10 20 30 40 50 60 MQLEKIQSDV EDIWKNRSKL SNRSVKRAAR VIIKKVIKLL DSGKIRVAER LSDGKWIVHT 70 80 90 100 110 120 WIKQAISLYF LTEESKMIDH TGWWFDKVNN KFDGWNEEEF HQSKIRAVPG CFVRQSAYVG 130 140 150 160 170 180 TNVVLMPSFI NVGAYISSGT MIDTWSTIGS CAQIGKNCHV SGGVGIGGVL EPIQASPVII 190 200 210 220 230 240 EDNCFIGARS EVAEGVIIRE GSVLGMGVFI GASTKIIDRE TNKVFYGEVP PYSVVVPGST 250 260 270 LSANNISIYC AIIVKKVDEK TRQKTSINEI LRD

References

[1]

"The Wolbachia genome of Brugia malayi: endosymbiont evolution within a human pathogenic nematode." Foster J., Ganatra M., Kamal I., Ware J., Makarova K., Ivanova N., Bhattacharyya A., Kapatral V., Kumar S., Posfai J., Vincze T., Ingram J., Moran L., Lapidus A., Omelchenko M., Kyrpides N., Ghedin E., Wang S. , Goltsman E., Joukov V., Ostrovskaya O., Tsukerman K., Mazur M., Comb D., Koonin E., Slatko B. PLoS Biol. 3:599-614(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TRS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017321 Genomic DNA. Translation: AAW71037.1. Different initiation.
RefSeq	YP_198279.1. NC_006833.1.
3D structure databases
ProteinModelPortal	Q5GSI7.
ModBase	Search...
Protein-protein interaction databases
STRING	292805.Wbm0449.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW71037; AAW71037; Wbm0449.
GeneID	3266322.
KEGG	wbm:Wbm0449.
PATRIC	24032663. VBIWolEnd7741_0705.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2171.
HOGENOM	HOG000003295.
KO	K00674.
OMA	NQWAKKA.
ProtClustDB	PRK11830.
Enzyme and pathway databases
BioCyc	WEND292805:GH18-513-MONOMER.
UniPathway	UPA00034; UER00019.
Family and domain databases
Gene3D	1.10.166.10. 1 hit.
HAMAP	MF_00811. DapD.
InterPro	IPR005664. DapD_Trfase_Hexpep_rpt_fam. IPR001451. Hexapep_transf. IPR023180. THP_succinylTrfase_dom1. IPR011004. Trimer_LpxA-like. [Graphical view]
PANTHER	PTHR19136:SF52. PTHR19136:SF52. 1 hit.
Pfam	PF00132. Hexapep. 2 hits. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR00965. dapD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPD_WOLTR

Accession

Primary (citable) accession number: Q5GSI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	May 5, 2009
Last modified:	May 29, 2013
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program