Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5GS31 (SYL_WOLTR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Wbm0605
OrganismWolbachia sp. subsp. Brugia malayi (strain TRS) [Complete proteome] [HAMAP]
Taxonomic identifier292805 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia

Protein attributes

Sequence length865 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

regulation of translational fidelity

Inferred from electronic annotation. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 865865Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009464

Regions

Motif36 – 4611"HIGH" region HAMAP-Rule MF_00049
Motif608 – 6125"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6111ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5GS31 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: CACA7BBB8CECF813

FASTA86598,820
        10         20         30         40         50         60 
MKYDFKNVEK FYQDRWDFSI GKNNEQGKCY VLEMFPYPSG KIHMGHLRNY VIGDVIARYK 

        70         80         90        100        110        120 
RACGFEVLHP IGWDAFGLPA ENAARDNNVN PAAWTKENID NMRAQLKSIG LSYNWERELS 

       130        140        150        160        170        180 
TCEADYYKHE QKFFLDFLKQ GLVYRKKSWV NWDPIDQTVL ANEQVVDGKG WRSGAIVEKR 

       190        200        210        220        230        240 
ELSQWFLKIT DFAEDLLECL QGLENWPEKV KMMQDRWIGK SEGVTIEFKI VGLNKKLKVF 

       250        260        270        280        290        300 
TTCPHTLFGA SFCAVAIEHP IVQDLMSKEI QDLISSIKIQ GKNNEKVGIY TGLNVKHPFL 

       310        320        330        340        350        360 
DKELPLYVAN FVLMEYREGA IFGCPAHDQR DFEFAQEYDL PIIPVISSAR LGIIPACDQG 

       370        380        390        400        410        420 
SYTGSQCQAT RMADGLNEEY TNNSIMFNSE FLNGLTVSEA RKVIVEKLEE KGIGKKTINY 

       430        440        450        460        470        480 
RLHDWGVSRQ RYWGCPIPVI YCKNCGTVPV PEEDLPVTLP TDVDFTSGGN PLDKHPTWKF 

       490        500        510        520        530        540 
VNCPKCKEQA ERETDTFDTF FESSWYFAAF CSENKSIDKN ACNRFMPVDY YIGGIEHAIL 

       550        560        570        580        590        600 
HLLYSRFFCR ALTKCGYFNV KEPFSTLITQ GMVCHVTYKD KNGKWLFPEE AKRLITQGAK 

       610        620        630        640        650        660 
IQVGKVEKMS KSKKNTVDPN FIIEKYGADT ARLFVLSDTP PEKDMEWSND GVEGCSRYIN 

       670        680        690        700        710        720 
KLWRMVMQFK PVVILNQSPV KQVADTEIQK EDMLSHAGMI PDKNVTGKFL GYRKKIHKLL 

       730        740        750        760        770        780 
HGLTDDLENC RLNCVVAKFR EMTNLIAEID VKTGKSLINE GICILIRVVE PFIPHLAESL 

       790        800        810        820        830        840 
WREIGGEGML YLQPWPKAAK SLLIDDVVTV AVQINGKLRA TIEVAINLHQ EELKQIAINS 

       850        860 
VSNRVDQSKV RAVYAVSNKI VNIVT 

« Hide

References

[1]"The Wolbachia genome of Brugia malayi: endosymbiont evolution within a human pathogenic nematode."
Foster J., Ganatra M., Kamal I., Ware J., Makarova K., Ivanova N., Bhattacharyya A., Kapatral V., Kumar S., Posfai J., Vincze T., Ingram J., Moran L., Lapidus A., Omelchenko M., Kyrpides N., Ghedin E., Wang S. expand/collapse author list , Goltsman E., Joukov V., Ostrovskaya O., Tsukerman K., Mazur M., Comb D., Koonin E., Slatko B.
PLoS Biol. 3:599-614(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TRS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017321 Genomic DNA. Translation: AAW71193.1.
RefSeqYP_198435.1. NC_006833.1.

3D structure databases

ProteinModelPortalQ5GS31.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING292805.Wbm0605.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAW71193; AAW71193; Wbm0605.
GeneID3266996.
KEGGwbm:Wbm0605.
PATRIC24033241. VBIWolEnd7741_0986.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycWEND292805:GH18-700-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_WOLTR
AccessionPrimary (citable) accession number: Q5GS31
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2005
Last modified: February 19, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries