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Protein

Carboxylesterase 5A

Gene

Ces5a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs.By similarity

Catalytic activityi

A carboxylic ester + H2O = an alcohol + a carboxylate.PROSITE-ProRule annotation

Kineticsi

  1. KM=670 µM for p-NPA1 Publication
  1. Vmax=200 µmol/min/mg enzyme with p-NPA as substrate1 Publication

pH dependencei

Optimum pH is 7.2.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei226Acyl-ester intermediatePROSITE-ProRule annotation1
Active sitei345Charge relay systemBy similarity1
Active sitei454Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Protein family/group databases

ESTHERiratno-cauxin. Carb_B_Chordata.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxylesterase 5A (EC:3.1.1.1)
Alternative name(s):
Carboxylesterase-like urinary excreted protein homolog
Short name:
Cauxin
Epididymis-specific gene 615 protein
Gene namesi
Name:Ces5a
Synonyms:Ces7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1549717. Ces5a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000030859329 – 575Carboxylesterase 5AAdd BLAST547

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi94 ↔ 121By similarity
Disulfide bondi280 ↔ 291By similarity
Glycosylationi281N-linked (GlcNAc...)Sequence analysis1
Glycosylationi363N-linked (GlcNAc...)Sequence analysis1
Glycosylationi515N-linked (GlcNAc...)Sequence analysis1
Glycosylationi524N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ5GRG2.

Expressioni

Gene expression databases

BgeeiENSRNOG00000025710.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000045129.

Structurei

3D structure databases

ProteinModelPortaliQ5GRG2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1516. Eukaryota.
COG2272. LUCA.
HOVERGENiHBG008839.
InParanoidiQ5GRG2.
KOiK15743.
PhylomeDBiQ5GRG2.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 2 hits.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5GRG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGDWVHTGQ ALIWVLWIFA AIIRGSVTEE PHRYTRLGWV QGKQATVLGR
60 70 80 90 100
LEPVNVFLGI PFAAPPLGPL RFSNPQPPIP WHDLREATTY PNVCFQNLEW
110 120 130 140 150
LFIYQNLLKV HYPKLGVSED CLYLNIYAPA YANDGSRLPV MMWIPGGGFE
160 170 180 190 200
TGSASIFDGS ALAAYEDVLI VTIQYRLGIF GFFNTQNQHA PGNWAFQDQL
210 220 230 240 250
AALQWVRENI NYFGGNPDSV TIFGGSAGAI SISSLILSPL SAGLFHRAIM
260 270 280 290 300
QSGVAIIPSL KNFDDELKHG LQVVADVCKC NVSDSKVLLK CLREKSSLEL
310 320 330 340 350
LSLGQKTKAF TRVVDGSFFP EEPMELLSQK TFKTVPSIIG VNNQECGYIL
360 370 380 390 400
PMREAPEILF GSNESTALTL IHVLLHIPPQ YMHIVAKDYF HGKHSLTDIR
410 420 430 440 450
DTLLDLFGDV FFVVPGLVTA RNHRDADGPV YFYEFQHRPN CFQNTRPAFV
460 470 480 490 500
KADHTDEIRF VFGGPFLEGD VVMFEEATED EKLLSRKMMS YWANFARSGD
510 520 530 540 550
PNGDDLPLWP AYDQNESYLK LDVNISTGWR LKDRRVEFWT DTLPLIMSAS
560 570
KALLSPTFPL ILFSLLPPSL LSIAS
Length:575
Mass (Da):64,417
Last modified:March 1, 2005 - v1
Checksum:i61E4404845225D47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF479659 mRNA. Translation: AAQ05814.1.
RefSeqiNP_001012056.1. NM_001012056.1.
UniGeneiRn.61848.

Genome annotation databases

GeneIDi307660.
KEGGirno:307660.
UCSCiRGD:1549717. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF479659 mRNA. Translation: AAQ05814.1.
RefSeqiNP_001012056.1. NM_001012056.1.
UniGeneiRn.61848.

3D structure databases

ProteinModelPortaliQ5GRG2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000045129.

Protein family/group databases

ESTHERiratno-cauxin. Carb_B_Chordata.

Proteomic databases

PaxDbiQ5GRG2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi307660.
KEGGirno:307660.
UCSCiRGD:1549717. rat.

Organism-specific databases

CTDi221223.
RGDi1549717. Ces5a.

Phylogenomic databases

eggNOGiKOG1516. Eukaryota.
COG2272. LUCA.
HOVERGENiHBG008839.
InParanoidiQ5GRG2.
KOiK15743.
PhylomeDBiQ5GRG2.

Miscellaneous databases

PROiQ5GRG2.

Gene expression databases

BgeeiENSRNOG00000025710.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 2 hits.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEST5A_RAT
AccessioniPrimary (citable) accession number: Q5GRG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: March 1, 2005
Last modified: October 5, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.