Phosphoenolpyruvate carboxylase 2 - Arabidopsis thaliana (Mouse-ear cress)

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Q5GM68 (CAPP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 68. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxylase 2
Short name=AtPPC2
Short name=PEPC 2
Short name=PEPCase 2
EC=4.1.1.31

Gene names

Name:	PPC2
Ordered Locus Names:	At2g42600
ORF Names:	F14N22.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	963 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.
Catalytic activity	Phosphate + oxaloacetate = H₂O + phosphoenolpyruvate + HCO₃^-.
Cofactor	Magnesium By similarity.
Enzyme regulation	By light-reversible phosphorylation By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed in all plant organs, with higher levels in stems and leaves. Ref.1
Post-translational modification	Ubiquitinated.
Sequence similarities	Belongs to the PEPCase type 1 family.
Sequence caution	The sequence AAD22994.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbon dioxide fixation Photosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Lyase
PTM	Phosphoprotein Ubl conjugation
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	apoplast Inferred from direct assay PubMed 18538804. Source: TAIR chloroplast Inferred from direct assay PubMed 18431481. Source: TAIR cytosol Inferred from direct assay Ref.5. Source: TAIR plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR
Molecular_function	phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 963

963

Phosphoenolpyruvate carboxylase 2

PRO_0000166658

Sites

Active site

172

By similarity

Active site

599

By similarity

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

701

Phosphoserine Ref.5

Experimental info

Sequence conflict

453

E → D in AAP43628. Ref.2

Sequence conflict

456

S → T in AAP43628. Ref.2

Sequence conflict

770

W → R in CAD58726. Ref.1

Sequence conflict

799

S → N in AAP43628. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q5GM68 [UniParc].

Last modified August 30, 2005. Version 2.
Checksum: D2F000BC8087D845
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FASTA

963

109,753

        10         20         30         40         50         60 
MAARNLEKMA SIDAQLRLLA PGKVSEDDKL IEYDALLLDR FLDILQDLHG EDVREFVQEC 

        70         80         90        100        110        120 
YEVAADYDGN RNTEKLEELG NMLTSLDPGD SIVVTKSFSN MLSLANLAEE VQIAYRRRIK 

       130        140        150        160        170        180 
KLKKGDFADE ASATTESDIE ETLKRLLQLN KTPEEVFDAL KNQTVDLVLT AHPTQSVRRS 

       190        200        210        220        230        240 
LLQKFGRIRD CLTQLYAKDI TPDDKQELDE ALQREIQAAF RTDEIRRTPP TPQDEMRAGM 

       250        260        270        280        290        300 
SYFHETIWKG VPKFLRRVDT ALKNIGINER VPYNAPLIQF SSWMGGDRDG NPRVTPEVTR 

       310        320        330        340        350        360 
DVCLLARMMA ANLYFSQIED LMFEMSMWRC NEELRVRAER QRCAKRDAKH YIEFWKQIPA 

       370        380        390        400        410        420 
NEPYRAILGD VRDKLYNTRE RARQLLSSGV SDVPEDAVFT SVDQFLEPLE LCYRSLCDCG 

       430        440        450        460        470        480 
DRPIADGSLL DFLRQVSTFG LALVKLDIRQ ESERHSDVLD AITTHLGIGS YKEWSEDKRQ 

       490        500        510        520        530        540 
EWLLSELSGK RPLFGPDLPK TEEVADVLDT FKVISELPSD SFGAYIISMA TAPSDVLAVE 

       550        560        570        580        590        600 
LLQRECGITD PLRVVPLFEK LADLESAPAA VARLFSIEWY RNRINGKQEV MIGYSDSGKD 

       610        620        630        640        650        660 
AGRLSAAWQL YKTQEELVKV AKEYGVKLTM FHGRGGTVGR GGGPTHLAIL SQPPDTIHGQ 

       670        680        690        700        710        720 
LRVTVQGEVI EQSFGEEHLC FRTLQRFTAA TLEHGMHPPV SPKPEWRVLM DEMAIIATEE 

       730        740        750        760        770        780 
YRSVVFKEPR FVEYFRLATP ELEYGRMNIG SRPSKRKPSG GIESLRAIPW IFAWTQTRFH 

       790        800        810        820        830        840 
LPVWLGFGGA FKRVIQKDSK NLNMLKEMYN QWPFFRVTID LVEMVFAKGD PGIAALYDRL 

       850        860        870        880        890        900 
LVSEELQPFG EQLRVNYQET RRLLLQVAGH KDILEGDPYL RQRLQLRDPY ITTLNVCQAY 

       910        920        930        940        950        960 
TLKQIRDPSF HVKVRPHLSK DYMESSPAAE LVKLNPKSEY APGLEDTVIL TMKGIAAGMQ 


NTG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and expression analysis of a gene encoding a bacterial-type phosphoenolpyruvate carboxylase from Arabidopsis and rice." Sanchez R., Cejudo F.J. Plant Physiol. 132:949-957(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Cloning of Arabidopsis guard cell PEP carboxylase." Zanor M.I., Plesch G., Mueller-Roeber B. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis." de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E., Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J., Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H. J. Proteome Res. 7:2458-2470(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-701, MASS SPECTROMETRY. Tissue: Root.
[6]	"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D. Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ532902 mRNA. Translation: CAD58726.1. AY210895 mRNA. Translation: AAP43628.1. AC007087 Genomic DNA. Translation: AAD22994.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC10145.1. CP002685 Genomic DNA. Translation: AEC10146.1.
IPI	IPI00530401.
PIR	H84855.
RefSeq	NP_850372.4. NM_180041.4. NP_850373.4. NM_180042.5.
UniGene	At.14366.
3D structure databases
ProteinModelPortal	Q5GM68.
SMR	Q5GM68. Positions 31-963.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G42600.2-P.
Proteomic databases
PaxDb	Q5GM68.
PRIDE	Q5GM68.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G42600.1; AT2G42600.1; AT2G42600. AT2G42600.2; AT2G42600.2; AT2G42600.
GeneID	818860.
KEGG	ath:AT2G42600.
Organism-specific databases
GeneFarm	5061. 479.
TAIR	At2g42600.
Phylogenomic databases
eggNOG	COG2352.
HOGENOM	HOG000238648.
InParanoid	Q5GM68.
KO	K01595.
OMA	VAGHKDI.
PhylomeDB	Q5GM68.
ProtClustDB	CLSN2679806.
Enzyme and pathway databases
BRENDA	4.1.1.31. 399.
Gene expression databases
ArrayExpress	Q5GM68.
Genevestigator	Q5GM68.
GermOnline	AT2G42600. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR021135. PEP_COase. IPR018129. PEP_COase_AS. IPR022805. PEP_COase_bac/pln-type. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Pfam	PF00311. PEPcase. 1 hit. [Graphical view]
PRINTS	PR00150. PEPCARBXLASE.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PROSITE	PS00781. PEPCASE_1. 1 hit. PS00393. PEPCASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPP2_ARATH

Accession

Primary (citable) accession number: Q5GM68
Secondary accession number(s): Q8GVE9, Q9SIN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 30, 2005
Last modified:	May 1, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents