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Q5GM68

- CAPP2_ARATH

UniProt

Q5GM68 - CAPP2_ARATH

Protein

Phosphoenolpyruvate carboxylase 2

Gene

PPC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.

    Catalytic activityi

    Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    By light-reversible phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei172 – 1721By similarity
    Active sitei599 – 5991By similarity

    GO - Molecular functioni

    1. phosphoenolpyruvate carboxylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbon fixation Source: UniProtKB-KW
    2. photosynthesis Source: UniProtKB-KW
    3. tricarboxylic acid cycle Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbon dioxide fixation, Photosynthesis

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciARA:GQT-2357-MONOMER.
    BRENDAi4.1.1.31. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxylase 2 (EC:4.1.1.31)
    Short name:
    AtPPC2
    Short name:
    PEPC 2
    Short name:
    PEPCase 2
    Gene namesi
    Name:PPC2
    Ordered Locus Names:At2g42600
    ORF Names:F14N22.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G42600.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. chloroplast Source: TAIR
    3. cytosol Source: TAIR
    4. plasma membrane Source: TAIR
    5. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 963963Phosphoenolpyruvate carboxylase 2PRO_0000166658Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei11 – 111PhosphoserineBy similarity
    Modified residuei701 – 7011Phosphoserine1 Publication

    Post-translational modificationi

    Ubiquitinated.1 Publication

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ5GM68.
    PRIDEiQ5GM68.

    Expressioni

    Tissue specificityi

    Expressed in all plant organs, with higher levels in stems and leaves.1 Publication

    Gene expression databases

    ArrayExpressiQ5GM68.
    GenevestigatoriQ5GM68.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    BioGridi4197. 2 interactions.
    STRINGi3702.AT2G42600.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5GM68.
    SMRiQ5GM68. Positions 6-963.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PEPCase type 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG2352.
    HOGENOMiHOG000238648.
    InParanoidiQ5GM68.
    KOiK01595.
    OMAiYNAPIIQ.
    PhylomeDBiQ5GM68.

    Family and domain databases

    HAMAPiMF_00595. PEPcase_type1.
    InterProiIPR021135. PEP_COase.
    IPR018129. PEP_COase_AS.
    IPR022805. PEP_COase_bac/pln-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PfamiPF00311. PEPcase. 1 hit.
    [Graphical view]
    PRINTSiPR00150. PEPCARBXLASE.
    SUPFAMiSSF51621. SSF51621. 1 hit.
    PROSITEiPS00781. PEPCASE_1. 1 hit.
    PS00393. PEPCASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5GM68-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAARNLEKMA SIDAQLRLLA PGKVSEDDKL IEYDALLLDR FLDILQDLHG    50
    EDVREFVQEC YEVAADYDGN RNTEKLEELG NMLTSLDPGD SIVVTKSFSN 100
    MLSLANLAEE VQIAYRRRIK KLKKGDFADE ASATTESDIE ETLKRLLQLN 150
    KTPEEVFDAL KNQTVDLVLT AHPTQSVRRS LLQKFGRIRD CLTQLYAKDI 200
    TPDDKQELDE ALQREIQAAF RTDEIRRTPP TPQDEMRAGM SYFHETIWKG 250
    VPKFLRRVDT ALKNIGINER VPYNAPLIQF SSWMGGDRDG NPRVTPEVTR 300
    DVCLLARMMA ANLYFSQIED LMFEMSMWRC NEELRVRAER QRCAKRDAKH 350
    YIEFWKQIPA NEPYRAILGD VRDKLYNTRE RARQLLSSGV SDVPEDAVFT 400
    SVDQFLEPLE LCYRSLCDCG DRPIADGSLL DFLRQVSTFG LALVKLDIRQ 450
    ESERHSDVLD AITTHLGIGS YKEWSEDKRQ EWLLSELSGK RPLFGPDLPK 500
    TEEVADVLDT FKVISELPSD SFGAYIISMA TAPSDVLAVE LLQRECGITD 550
    PLRVVPLFEK LADLESAPAA VARLFSIEWY RNRINGKQEV MIGYSDSGKD 600
    AGRLSAAWQL YKTQEELVKV AKEYGVKLTM FHGRGGTVGR GGGPTHLAIL 650
    SQPPDTIHGQ LRVTVQGEVI EQSFGEEHLC FRTLQRFTAA TLEHGMHPPV 700
    SPKPEWRVLM DEMAIIATEE YRSVVFKEPR FVEYFRLATP ELEYGRMNIG 750
    SRPSKRKPSG GIESLRAIPW IFAWTQTRFH LPVWLGFGGA FKRVIQKDSK 800
    NLNMLKEMYN QWPFFRVTID LVEMVFAKGD PGIAALYDRL LVSEELQPFG 850
    EQLRVNYQET RRLLLQVAGH KDILEGDPYL RQRLQLRDPY ITTLNVCQAY 900
    TLKQIRDPSF HVKVRPHLSK DYMESSPAAE LVKLNPKSEY APGLEDTVIL 950
    TMKGIAAGMQ NTG 963
    Length:963
    Mass (Da):109,753
    Last modified:August 30, 2005 - v2
    Checksum:iD2F000BC8087D845
    GO

    Sequence cautioni

    The sequence AAD22994.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti453 – 4531E → D in AAP43628. 1 PublicationCurated
    Sequence conflicti456 – 4561S → T in AAP43628. 1 PublicationCurated
    Sequence conflicti770 – 7701W → R in CAD58726. (PubMed:12805623)Curated
    Sequence conflicti799 – 7991S → N in AAP43628. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ532902 mRNA. Translation: CAD58726.1.
    AY210895 mRNA. Translation: AAP43628.1.
    AC007087 Genomic DNA. Translation: AAD22994.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC10145.1.
    CP002685 Genomic DNA. Translation: AEC10146.1.
    PIRiH84855.
    RefSeqiNP_850372.4. NM_180041.4.
    NP_850373.4. NM_180042.5.
    UniGeneiAt.14366.

    Genome annotation databases

    EnsemblPlantsiAT2G42600.1; AT2G42600.1; AT2G42600.
    AT2G42600.2; AT2G42600.2; AT2G42600.
    GeneIDi818860.
    KEGGiath:AT2G42600.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ532902 mRNA. Translation: CAD58726.1 .
    AY210895 mRNA. Translation: AAP43628.1 .
    AC007087 Genomic DNA. Translation: AAD22994.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC10145.1 .
    CP002685 Genomic DNA. Translation: AEC10146.1 .
    PIRi H84855.
    RefSeqi NP_850372.4. NM_180041.4.
    NP_850373.4. NM_180042.5.
    UniGenei At.14366.

    3D structure databases

    ProteinModelPortali Q5GM68.
    SMRi Q5GM68. Positions 6-963.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4197. 2 interactions.
    STRINGi 3702.AT2G42600.2-P.

    Proteomic databases

    PaxDbi Q5GM68.
    PRIDEi Q5GM68.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G42600.1 ; AT2G42600.1 ; AT2G42600 .
    AT2G42600.2 ; AT2G42600.2 ; AT2G42600 .
    GeneIDi 818860.
    KEGGi ath:AT2G42600.

    Organism-specific databases

    GeneFarmi 5061. 479.
    TAIRi AT2G42600.

    Phylogenomic databases

    eggNOGi COG2352.
    HOGENOMi HOG000238648.
    InParanoidi Q5GM68.
    KOi K01595.
    OMAi YNAPIIQ.
    PhylomeDBi Q5GM68.

    Enzyme and pathway databases

    BioCyci ARA:GQT-2357-MONOMER.
    BRENDAi 4.1.1.31. 399.

    Gene expression databases

    ArrayExpressi Q5GM68.
    Genevestigatori Q5GM68.

    Family and domain databases

    HAMAPi MF_00595. PEPcase_type1.
    InterProi IPR021135. PEP_COase.
    IPR018129. PEP_COase_AS.
    IPR022805. PEP_COase_bac/pln-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    Pfami PF00311. PEPcase. 1 hit.
    [Graphical view ]
    PRINTSi PR00150. PEPCARBXLASE.
    SUPFAMi SSF51621. SSF51621. 1 hit.
    PROSITEi PS00781. PEPCASE_1. 1 hit.
    PS00393. PEPCASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and expression analysis of a gene encoding a bacterial-type phosphoenolpyruvate carboxylase from Arabidopsis and rice."
      Sanchez R., Cejudo F.J.
      Plant Physiol. 132:949-957(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, NOMENCLATURE.
      Strain: cv. Columbia.
    2. "Cloning of Arabidopsis guard cell PEP carboxylase."
      Zanor M.I., Plesch G., Mueller-Roeber B.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-701, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Root.
    6. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
      Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
      Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCAPP2_ARATH
    AccessioniPrimary (citable) accession number: Q5GM68
    Secondary accession number(s): Q8GVE9, Q9SIN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3