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Protein

Phosphoenolpyruvate carboxylase 2

Gene

PPC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.

Cofactori

Mg2+By similarity

Enzyme regulationi

By light-reversible phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei172 – 1721By similarity
Active sitei599 – 5991By similarity

GO - Molecular functioni

  1. phosphoenolpyruvate carboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbon fixation Source: UniProtKB-KW
  2. photosynthesis Source: UniProtKB-KW
  3. tricarboxylic acid cycle Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbon dioxide fixation, Photosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciARA:GQT-2357-MONOMER.
BRENDAi4.1.1.31. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxylase 2 (EC:4.1.1.31)
Short name:
AtPPC2
Short name:
PEPC 2
Short name:
PEPCase 2
Gene namesi
Name:PPC2
Ordered Locus Names:At2g42600
ORF Names:F14N22.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G42600.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. chloroplast Source: TAIR
  3. cytosol Source: TAIR
  4. plasma membrane Source: TAIR
  5. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 963963Phosphoenolpyruvate carboxylase 2PRO_0000166658Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111PhosphoserineBy similarity
Modified residuei701 – 7011Phosphoserine1 Publication

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5GM68.
PRIDEiQ5GM68.

Expressioni

Tissue specificityi

Expressed in all plant organs, with higher levels in stems and leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ5GM68. baseline and differential.
GenevestigatoriQ5GM68.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi4197. 2 interactions.
STRINGi3702.AT2G42600.2-P.

Structurei

3D structure databases

ProteinModelPortaliQ5GM68.
SMRiQ5GM68. Positions 6-963.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PEPCase type 1 family.Curated

Phylogenomic databases

eggNOGiCOG2352.
HOGENOMiHOG000238648.
InParanoidiQ5GM68.
KOiK01595.
OMAiRANYEEN.
PhylomeDBiQ5GM68.

Family and domain databases

HAMAPiMF_00595. PEPcase_type1.
InterProiIPR021135. PEP_COase.
IPR018129. PEP_COase_AS.
IPR022805. PEP_COase_bac/pln-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00311. PEPcase. 1 hit.
[Graphical view]
PRINTSiPR00150. PEPCARBXLASE.
SUPFAMiSSF51621. SSF51621. 1 hit.
PROSITEiPS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5GM68-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAARNLEKMA SIDAQLRLLA PGKVSEDDKL IEYDALLLDR FLDILQDLHG
60 70 80 90 100
EDVREFVQEC YEVAADYDGN RNTEKLEELG NMLTSLDPGD SIVVTKSFSN
110 120 130 140 150
MLSLANLAEE VQIAYRRRIK KLKKGDFADE ASATTESDIE ETLKRLLQLN
160 170 180 190 200
KTPEEVFDAL KNQTVDLVLT AHPTQSVRRS LLQKFGRIRD CLTQLYAKDI
210 220 230 240 250
TPDDKQELDE ALQREIQAAF RTDEIRRTPP TPQDEMRAGM SYFHETIWKG
260 270 280 290 300
VPKFLRRVDT ALKNIGINER VPYNAPLIQF SSWMGGDRDG NPRVTPEVTR
310 320 330 340 350
DVCLLARMMA ANLYFSQIED LMFEMSMWRC NEELRVRAER QRCAKRDAKH
360 370 380 390 400
YIEFWKQIPA NEPYRAILGD VRDKLYNTRE RARQLLSSGV SDVPEDAVFT
410 420 430 440 450
SVDQFLEPLE LCYRSLCDCG DRPIADGSLL DFLRQVSTFG LALVKLDIRQ
460 470 480 490 500
ESERHSDVLD AITTHLGIGS YKEWSEDKRQ EWLLSELSGK RPLFGPDLPK
510 520 530 540 550
TEEVADVLDT FKVISELPSD SFGAYIISMA TAPSDVLAVE LLQRECGITD
560 570 580 590 600
PLRVVPLFEK LADLESAPAA VARLFSIEWY RNRINGKQEV MIGYSDSGKD
610 620 630 640 650
AGRLSAAWQL YKTQEELVKV AKEYGVKLTM FHGRGGTVGR GGGPTHLAIL
660 670 680 690 700
SQPPDTIHGQ LRVTVQGEVI EQSFGEEHLC FRTLQRFTAA TLEHGMHPPV
710 720 730 740 750
SPKPEWRVLM DEMAIIATEE YRSVVFKEPR FVEYFRLATP ELEYGRMNIG
760 770 780 790 800
SRPSKRKPSG GIESLRAIPW IFAWTQTRFH LPVWLGFGGA FKRVIQKDSK
810 820 830 840 850
NLNMLKEMYN QWPFFRVTID LVEMVFAKGD PGIAALYDRL LVSEELQPFG
860 870 880 890 900
EQLRVNYQET RRLLLQVAGH KDILEGDPYL RQRLQLRDPY ITTLNVCQAY
910 920 930 940 950
TLKQIRDPSF HVKVRPHLSK DYMESSPAAE LVKLNPKSEY APGLEDTVIL
960
TMKGIAAGMQ NTG
Length:963
Mass (Da):109,753
Last modified:August 30, 2005 - v2
Checksum:iD2F000BC8087D845
GO

Sequence cautioni

The sequence AAD22994.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti453 – 4531E → D in AAP43628. 1 PublicationCurated
Sequence conflicti456 – 4561S → T in AAP43628. 1 PublicationCurated
Sequence conflicti770 – 7701W → R in CAD58726. (PubMed:12805623)Curated
Sequence conflicti799 – 7991S → N in AAP43628. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ532902 mRNA. Translation: CAD58726.1.
AY210895 mRNA. Translation: AAP43628.1.
AC007087 Genomic DNA. Translation: AAD22994.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10145.1.
CP002685 Genomic DNA. Translation: AEC10146.1.
PIRiH84855.
RefSeqiNP_850372.4. NM_180041.4.
NP_850373.4. NM_180042.5.
UniGeneiAt.14366.

Genome annotation databases

EnsemblPlantsiAT2G42600.1; AT2G42600.1; AT2G42600.
AT2G42600.2; AT2G42600.2; AT2G42600.
GeneIDi818860.
KEGGiath:AT2G42600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ532902 mRNA. Translation: CAD58726.1.
AY210895 mRNA. Translation: AAP43628.1.
AC007087 Genomic DNA. Translation: AAD22994.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10145.1.
CP002685 Genomic DNA. Translation: AEC10146.1.
PIRiH84855.
RefSeqiNP_850372.4. NM_180041.4.
NP_850373.4. NM_180042.5.
UniGeneiAt.14366.

3D structure databases

ProteinModelPortaliQ5GM68.
SMRiQ5GM68. Positions 6-963.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4197. 2 interactions.
STRINGi3702.AT2G42600.2-P.

Proteomic databases

PaxDbiQ5GM68.
PRIDEiQ5GM68.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G42600.1; AT2G42600.1; AT2G42600.
AT2G42600.2; AT2G42600.2; AT2G42600.
GeneIDi818860.
KEGGiath:AT2G42600.

Organism-specific databases

GeneFarmi5061. 479.
TAIRiAT2G42600.

Phylogenomic databases

eggNOGiCOG2352.
HOGENOMiHOG000238648.
InParanoidiQ5GM68.
KOiK01595.
OMAiRANYEEN.
PhylomeDBiQ5GM68.

Enzyme and pathway databases

BioCyciARA:GQT-2357-MONOMER.
BRENDAi4.1.1.31. 399.

Gene expression databases

ExpressionAtlasiQ5GM68. baseline and differential.
GenevestigatoriQ5GM68.

Family and domain databases

HAMAPiMF_00595. PEPcase_type1.
InterProiIPR021135. PEP_COase.
IPR018129. PEP_COase_AS.
IPR022805. PEP_COase_bac/pln-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00311. PEPcase. 1 hit.
[Graphical view]
PRINTSiPR00150. PEPCARBXLASE.
SUPFAMiSSF51621. SSF51621. 1 hit.
PROSITEiPS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and expression analysis of a gene encoding a bacterial-type phosphoenolpyruvate carboxylase from Arabidopsis and rice."
    Sanchez R., Cejudo F.J.
    Plant Physiol. 132:949-957(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "Cloning of Arabidopsis guard cell PEP carboxylase."
    Zanor M.I., Plesch G., Mueller-Roeber B.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-701, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Root.
  6. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
    Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
    Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCAPP2_ARATH
AccessioniPrimary (citable) accession number: Q5GM68
Secondary accession number(s): Q8GVE9, Q9SIN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: January 7, 2015
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.