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Protein

Glycerol-3-phosphate acyltransferase 1, mitochondrial

Gene

GPAM

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.By similarity

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 4 (GPAT4), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (AGPAT4)
  3. Phosphatidate cytidylyltransferase, mitochondrial (TAMM41), Phosphatidate cytidylyltransferase 2 (CDS2), Phosphatidate cytidylyltransferase, mitochondrial (TAMM41), Phosphatidate cytidylyltransferase (CDS1)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferase 1, mitochondrial (EC:2.3.1.15)
Short name:
GPAT-1
Gene namesi
Name:GPAM
Synonyms:GPAT1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini? – 470Mitochondrial intermembraneSequence analysis
Transmembranei471 – 493HelicalSequence analysisAdd BLAST23
Topological domaini494 – 573CytoplasmicSequence analysisAdd BLAST80
Transmembranei574 – 592HelicalSequence analysisAdd BLAST19
Topological domaini593 – 825Mitochondrial intermembraneSequence analysisAdd BLAST233

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000245029? – 825Glycerol-3-phosphate acyltransferase 1, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei380PhosphoserineBy similarity1
Modified residuei685PhosphoserineBy similarity1
Modified residuei692PhosphoserineBy similarity1
Modified residuei777N6-acetyllysineBy similarity1
Modified residuei781N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5GJ77.
PRIDEiQ5GJ77.

Expressioni

Tissue specificityi

Highly expressed in adipose tissues and lung. Low expression in liver.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015811.

Structurei

3D structure databases

ProteinModelPortaliQ5GJ77.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi230 – 235HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Belongs to the GPAT/DAPAT family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3729. Eukaryota.
COG2937. LUCA.
HOGENOMiHOG000112780.
InParanoidiQ5GJ77.
KOiK00629.

Family and domain databases

InterProiView protein in InterPro
IPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
PANTHERiPTHR12563. PTHR12563. 1 hit.
PfamiView protein in Pfam
PF01553. Acyltransferase. 1 hit.
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiView protein in SMART
SM00563. PlsC. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5GJ77-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDESALTLGT IDVSYLPNSS EYSIGRCKHA TEEWGECGSR PTVFRSATLK
60 70 80 90 100
WKESLMSRKR PFVGRCCYSC TPQSWDKFFN PSIPSLGLRN VIYINETHTR
110 120 130 140 150
HRGWLARRLS YVLFIQERDV HKGMFATNVT ENVLNSSRVQ EAIAEVAGEL
160 170 180 190 200
NPDGSAQQQS KAVNKVKKKA RKILQEMVAT VSPAMIRLTG WVLLKLFNSF
210 220 230 240 250
FWNIQIHKGQ LEMVKAATET NLPLIFLPVH RSHIDYLLLT FILFCHNIKA
260 270 280 290 300
PYIASGNNLN IPIFSTLIHK LGGFFIRRRL DETPDGRKDI LYRALLHGHI
310 320 330 340 350
VELLRQQQFL EIFLEGTRSR SGKISCARAG LLSVVVDTLS TNTIPDILII
360 370 380 390 400
PGGISYDRII EGHYNGEQLG KPKKNESLWS IARGVIRMLR KNYGCVKTDF
410 420 430 440 450
AQPFSLKEYL ESQSQKPVSA PLSLEQALLP AILPSRPSGA ADEGTDMSIN
460 470 480 490 500
ESRNATDESR RRLIAHLAEH ILFTASKSCA IMSTHIVACL LLYRHRQGIG
510 520 530 540 550
LFTLVEDFFV MKEEVLARDF DLGFSGNSED VVMHAIQFLG NCITITHTSK
560 570 580 590 600
NDEFFITPST TIPSVFELNF YSNGVLHVFI MEAIIACSLY AVLKKRGPGG
610 620 630 640 650
PASPSLVSQE QLVHKAASLC YLLSNEGTIS LPCQTFYQIC HETVGRFIQY
660 670 680 690 700
GILIVAEQDD QEDISPGLAE QQWDKKLPEP LSWRSDEEDE DSDFGEEQRD
710 720 730 740 750
CYLKVSQSKE HQQFITFLQR LLGPLLEAYS SAAVFIHNFG GPVPEPEFLQ
760 770 780 790 800
KLHKYLITRT ERRVAVYAES ATYCLVKNAV KTFKDIGVFK ETKQKRVSGL
810 820
ELSNTFLPQC NRQKLLEYIL SLVVL
Length:825
Mass (Da):93,144
Last modified:March 1, 2005 - v1
Checksum:i01D294B9DE03CC04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY515690 mRNA. Translation: AAS79429.1.
AY945228, AY945226, AY945227 Genomic DNA. Translation: AAY24765.1.
RefSeqiNP_001012282.1. NM_001012282.1.
UniGeneiBt.29570.

Genome annotation databases

GeneIDi497202.
KEGGibta:497202.

Similar proteinsi

Entry informationi

Entry nameiGPAT1_BOVIN
AccessioniPrimary (citable) accession number: Q5GJ77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 1, 2005
Last modified: August 30, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families