Q5GJ77 (GPAT1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase 1, mitochondrial
Short name=GPAT-1
EC=2.3.1.15

Gene names

Name:	GPAM
Synonyms:	GPAT1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	825 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis By similarity.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Mitochondrion outer membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Highly expressed in adipose tissues and lung. Low expression in liver. Ref.1
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transit peptide Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 825		Glycerol-3-phosphate acyltransferase 1, mitochondrial		PRO_0000245029
Regions
	Topological domain	? – 470		Mitochondrial intermembrane Potential
	Transmembrane	471 – 493	23	Helical; Potential
	Topological domain	494 – 573	80	Cytoplasmic Potential
	Transmembrane	574 – 592	19	Helical; Potential
	Topological domain	593 – 825	233	Mitochondrial intermembrane Potential
	Motif	230 – 235	6	HXXXXD motif
Amino acid modifications
	Modified residue	111	1	Phosphotyrosine By similarity
	Modified residue	692	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5GJ77 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 01D294B9DE03CC04	FASTA	825	93,144
	10 20 30 40 50 60 MDESALTLGT IDVSYLPNSS EYSIGRCKHA TEEWGECGSR PTVFRSATLK WKESLMSRKR 70 80 90 100 110 120 PFVGRCCYSC TPQSWDKFFN PSIPSLGLRN VIYINETHTR HRGWLARRLS YVLFIQERDV 130 140 150 160 170 180 HKGMFATNVT ENVLNSSRVQ EAIAEVAGEL NPDGSAQQQS KAVNKVKKKA RKILQEMVAT 190 200 210 220 230 240 VSPAMIRLTG WVLLKLFNSF FWNIQIHKGQ LEMVKAATET NLPLIFLPVH RSHIDYLLLT 250 260 270 280 290 300 FILFCHNIKA PYIASGNNLN IPIFSTLIHK LGGFFIRRRL DETPDGRKDI LYRALLHGHI 310 320 330 340 350 360 VELLRQQQFL EIFLEGTRSR SGKISCARAG LLSVVVDTLS TNTIPDILII PGGISYDRII 370 380 390 400 410 420 EGHYNGEQLG KPKKNESLWS IARGVIRMLR KNYGCVKTDF AQPFSLKEYL ESQSQKPVSA 430 440 450 460 470 480 PLSLEQALLP AILPSRPSGA ADEGTDMSIN ESRNATDESR RRLIAHLAEH ILFTASKSCA 490 500 510 520 530 540 IMSTHIVACL LLYRHRQGIG LFTLVEDFFV MKEEVLARDF DLGFSGNSED VVMHAIQFLG 550 560 570 580 590 600 NCITITHTSK NDEFFITPST TIPSVFELNF YSNGVLHVFI MEAIIACSLY AVLKKRGPGG 610 620 630 640 650 660 PASPSLVSQE QLVHKAASLC YLLSNEGTIS LPCQTFYQIC HETVGRFIQY GILIVAEQDD 670 680 690 700 710 720 QEDISPGLAE QQWDKKLPEP LSWRSDEEDE DSDFGEEQRD CYLKVSQSKE HQQFITFLQR 730 740 750 760 770 780 LLGPLLEAYS SAAVFIHNFG GPVPEPEFLQ KLHKYLITRT ERRVAVYAES ATYCLVKNAV 790 800 810 820 KTFKDIGVFK ETKQKRVSGL ELSNTFLPQC NRQKLLEYIL SLVVL

References

[1]

"Genomic structure and an alternative transcript of bovine mitochondrial glycerol-3-phosphate acyltransferase gene (GPAM)." Roy R., Ordovas L., Taourit S., Zaragoza P., Eggen A., Rodellar C. Cytogenet. Genome Res. 112:82-89(2006) [PubMed: 16276094] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY515690 mRNA. Translation: AAS79429.1. AY945228, AY945226, AY945227 Genomic DNA. Translation: AAY24765.1.
IPI	IPI00703084.
RefSeq	NP_001012282.1. NM_001012282.1.
UniGene	Bt.29570.
3D structure databases
ProteinModelPortal	Q5GJ77.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5GJ77.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	497202.
KEGG	bta:497202.
Organism-specific databases
CTD	57678.
Phylogenomic databases
eggNOG	maNOG04795.
GeneTree	ENSGT00520000055570.
InParanoid	Q5GJ77.
OrthoDB	EOG4BZN1X.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00629.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPAT1_BOVIN

Accession

Primary (citable) accession number: Q5GJ77

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	March 1, 2005
Last modified:	November 16, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program