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Protein

Vascular endothelial growth factor receptor 2

Gene

kdr

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. Combinations of multiple VEGF receptors are required for development of different blood vessel types in the embryo. Involved in angiogenesis, specifically in VEGF-induced sprouting of new blood vessels. Particularly involved in artery formation. Does not appear to be required for hematopoiesis.2 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei877 – 8771ATPPROSITE-ProRule annotation
Active sitei1039 – 10391Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi849 – 8579ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Angiogenesis, Differentiation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DRE-194306. Neurophilin interactions with VEGF and VEGFR.
R-DRE-195399. VEGF binds to VEGFR leading to receptor dimerization.
R-DRE-216083. Integrin cell surface interactions.
R-DRE-3928663. EPHA-mediated growth cone collapse.
R-DRE-4420097. VEGFA-VEGFR2 Pathway.
R-DRE-5218921. VEGFR2 mediated cell proliferation.

Names & Taxonomyi

Protein namesi
Recommended name:
Vascular endothelial growth factor receptor 2 (EC:2.7.10.1)
Short name:
VEGFR-2
Alternative name(s):
Fetal liver kinase 1b
Short name:
FLK-1b
Kinase insert domain receptor
Kinase insert domain receptor-B
Protein-tyrosine kinase receptor flk-1b
Vascular endothelial growth factor receptor 2 homolog B
Short name:
VEGFR-2 homolog B
Gene namesi
Name:kdr
Synonyms:flk1b, kdrb
ORF Names:si:busm1-205d10.1, si:ch211-254j6.1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 20

Organism-specific databases

ZFINiZDB-GENE-041001-112. kdr.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 774752ExtracellularSequence analysisAdd
BLAST
Transmembranei775 – 79521HelicalSequence analysisAdd
BLAST
Topological domaini796 – 1357562CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Membrane, Nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2331049.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 13571335Vascular endothelial growth factor receptor 2PRO_0000249465Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence analysis
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence analysis
Disulfide bondi53 ↔ 104PROSITE-ProRule annotation
Glycosylationi66 – 661N-linked (GlcNAc...)Sequence analysis
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence analysis
Disulfide bondi153 ↔ 203PROSITE-ProRule annotation
Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence analysis
Glycosylationi209 – 2091N-linked (GlcNAc...)Sequence analysis
Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence analysis
Disulfide bondi248 ↔ 314PROSITE-ProRule annotation
Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence analysis
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence analysis
Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence analysis
Glycosylationi407 – 4071N-linked (GlcNAc...)Sequence analysis
Disulfide bondi457 ↔ 538PROSITE-ProRule annotation
Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence analysis
Glycosylationi560 – 5601N-linked (GlcNAc...)Sequence analysis
Disulfide bondi579 ↔ 651PROSITE-ProRule annotation
Glycosylationi621 – 6211N-linked (GlcNAc...)Sequence analysis
Glycosylationi631 – 6311N-linked (GlcNAc...)Sequence analysis
Glycosylationi640 – 6401N-linked (GlcNAc...)Sequence analysis
Glycosylationi681 – 6811N-linked (GlcNAc...)Sequence analysis
Glycosylationi688 – 6881N-linked (GlcNAc...)Sequence analysis
Disulfide bondi697 ↔ 746PROSITE-ProRule annotation
Glycosylationi713 – 7131N-linked (GlcNAc...)Sequence analysis
Modified residuei1065 – 10651Phosphotyrosine; by autocatalysisBy similarity
Modified residuei1070 – 10701Phosphotyrosine; by autocatalysisBy similarity
Modified residuei1186 – 11861Phosphotyrosine; by autocatalysisBy similarity
Modified residuei1222 – 12221Phosphotyrosine; by autocatalysisBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ5GIT4.
PRIDEiQ5GIT4.

Expressioni

Tissue specificityi

First expressed in embryos between 5- and 7-somites in the bilateral stripes that contain the developing angioblasts, and then localized to the intermediate cell mass (ICM) and the developing vasculature. By 30 hpf, expressed in the major trunk, head and intersomitic vessels, persisting through 4 dpf when expression is seen in developing subintestinal veins and in the remaining vasculature.3 Publications

Developmental stagei

The first vegfr expressed during development.1 Publication

Gene expression databases

BgeeiENSDARG00000017321.

Interactioni

Subunit structurei

Interacts with isoform VEGF165 of vegfaa and, to a lesser extent, with isoform VEGF171 of vegfab.1 Publication

Protein-protein interaction databases

STRINGi7955.ENSDARP00000049203.

Chemistry

BindingDBiQ5GIT4.

Structurei

3D structure databases

ProteinModelPortaliQ5GIT4.
SMRiQ5GIT4. Positions 825-1181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 12089Ig-like C2-type 1Add
BLAST
Domaini120 – 222103Ig-like C2-type 2Add
BLAST
Domaini216 – 330115Ig-like C2-type 3Add
BLAST
Domaini335 – 42692Ig-like C2-type 4Add
BLAST
Domaini433 – 553121Ig-like C2-type 5Add
BLAST
Domaini556 – 667112Ig-like C2-type 6Add
BLAST
Domaini676 – 76287Ig-like C2-type 7Add
BLAST
Domaini843 – 1173331Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0200. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118923.
HOGENOMiHOG000037949.
HOVERGENiHBG053432.
InParanoidiQ5GIT4.
KOiK05098.
OMAiPSYMISY.
OrthoDBiEOG091G01TL.
PhylomeDBiQ5GIT4.
TreeFamiTF325768.

Family and domain databases

Gene3Di2.60.40.10. 7 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR001824. Tyr_kinase_rcpt_3_CS.
IPR009136. VEGFR2_rcpt.
IPR009137. VEGFR3_rcpt.
[Graphical view]
PANTHERiPTHR24416:SF45. PTHR24416:SF45. 3 hits.
PfamiPF07679. I-set. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR01835. VEGFRECEPTR3.
SMARTiSM00409. IG. 7 hits.
SM00408. IGc2. 4 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 8 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50835. IG_LIKE. 5 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5GIT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTSYALLL LDILLTFNVA KAIELRFVPD PPTLNITEKT IKINASDTLQ
60 70 80 90 100
ITCRGRQILE WSTPHNRTSS ETRLTISDCS GDGLFCSTLT LSKAVANETG
110 120 130 140 150
EYRCFYKSLP KEDGKTSVAV YVFIQDYRTP FVRIAQDYDV VFIREGEQVV
160 170 180 190 200
IPCLVSVEDL NVTLYTKYPV KELSTDGKEV IWDSRRGFIL PSRVVSYAGV
210 220 230 240 250
VYCQTTIRNE TFQSSPYIVA VVGYKIYDLT LSPQHERLTV GERLILNCTA
260 270 280 290 300
HTELNVGIDF QWTFPHEKRS VNGSMSTSRY KTSSNKKKLW NSLELSNTLT
310 320 330 340 350
VENVTLNDTG EYICTASSGQ MQKIAQASLI VYEKPFIALS DQLWQTVEAK
360 370 380 390 400
AGDAEAKILV KYYAYPEPAV RWYKNDQLIV LRDEYRMKFY RGVHLTIYGV
410 420 430 440 450
TEKDAGNYTV VMTNKITKEE QRRTFQLVVN DLPRIFEKDV SLDRDVHMYG
460 470 480 490 500
SSPTLTCTAS GGSSPVTIKW QWMPREDCPV RFLPKSDTRM AKCDKWREMS
510 520 530 540 550
NNTGKNPLIS QTSVDERTLK TISTLKIQKA VDHALYRCIA TNKMGQDQRV
560 570 580 590 600
IVFQVTRFLN LSVLPSSSPI EGQDVIMRCV ADRLLYYNLR WYRVANVANH
610 620 630 640 650
DPPPAAVPCD TLTLSHLHQP NVTVSGLQGT NVTLDMPIPN ATMMDQGLYA
660 670 680 690 700
CQVEIVGTNE KTCLLHNLRL RALEMSRIVT NLTDQRVNVS DSTTLVCEVS
710 720 730 740 750
GTPTPTIVWT KDNQTVMEGS GVILKRSNRV LTIQRVKKED SGLYICTACN
760 770 780 790 800
QQGCESSEAR ISVDGAEEKM NVELIMPIGA VVIAMFLWLL IVFVIRNRKR
810 820 830 840 850
PNDGDLKTGY LSIILDSDDM PMDEHCERLT YDASKWEFPR DRLKLGEPLG
860 870 880 890 900
RGAFGQVVEA TAYGIEKATT CTTVAVKMLK EGATSSEYRA LMSELKILIH
910 920 930 940 950
IGHHLNVVNL LGACTKQGGP LMVIVEYCKH GNLSSYLKSK RGEYSPYKKR
960 970 980 990 1000
TPRMPNRREV QQDEDPREGD LGLGTSTRLD ICTGTAVCTR TGEQTYKTLQ
1010 1020 1030 1040 1050
DEQESSDWDH LTMEDLISYS FQVAKGMEFL ASRKCIHRDL AARNILLSEN
1060 1070 1080 1090 1100
SVVKICDFGL ARDVYKDPDY VRKGDARLPL KWMAPETIFD RVYTTQSDVW
1110 1120 1130 1140 1150
SFGVLLWEIF SLGASPYPGV CIDESFCRRL KEGTRMRAPD YATPEIYQTM
1160 1170 1180 1190 1200
LDCWLDRPLD RPTFTQLVEH LGNLLQASAQ QDGKDYIPLT NGEMEEELVA
1210 1220 1230 1240 1250
PHLNVTSKRS FYAGNTEAQL HYDNAPPLGF PQQMNSSGVP VNMTGFVDIP
1260 1270 1280 1290 1300
LEHTTVMDGH VDCGVGLSRE QMKALDRQAQ RPLNFSPLLR CKSKESLASE
1310 1320 1330 1340 1350
SSNQTSGYQS GYHSDDAEAP IYANEEMILK RDIRKKPPLP KRNDKFSAEV

RYSAPPV
Length:1,357
Mass (Da):153,042
Last modified:October 14, 2008 - v2
Checksum:i43B97B484DBEA38F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171F → V in AAI63563 (PubMed:23594743).Curated
Sequence conflicti72 – 721T → S in AAI63563 (PubMed:23594743).Curated
Sequence conflicti109 – 1091L → P in AAY89336 (PubMed:17698971).Curated
Sequence conflicti254 – 2541L → R in AAY89336 (PubMed:17698971).Curated
Sequence conflicti262 – 2621W → C in AAI63563 (PubMed:23594743).Curated
Sequence conflicti287 – 2871K → R in AAS92270 (Ref. 1) Curated
Sequence conflicti384 – 3841E → G in AAY89336 (PubMed:17698971).Curated
Sequence conflicti459 – 4591A → G in AAY89336 (PubMed:17698971).Curated
Sequence conflicti488 – 4892TR → PS in AAY89336 (PubMed:17698971).Curated
Sequence conflicti488 – 4892TR → PS in AAI63563 (PubMed:23594743).Curated
Sequence conflicti553 – 5531F → S in AAS92270 (Ref. 1) Curated
Sequence conflicti958 – 9581R → K in AAS92270 (Ref. 1) Curated
Sequence conflicti996 – 9961Y → S in AAY89336 (PubMed:17698971).Curated
Sequence conflicti996 – 9961Y → S in AAI63563 (PubMed:23594743).Curated
Sequence conflicti996 – 9961Y → S in AAV93319 (PubMed:16617120).Curated
Sequence conflicti997 – 9971K → T in AAY89336 (PubMed:17698971).Curated
Sequence conflicti997 – 9971K → T in AAI63563 (PubMed:23594743).Curated
Sequence conflicti1100 – 11001W → R in AAS92270 (Ref. 1) Curated
Sequence conflicti1223 – 12231D → G in AAY89336 (PubMed:17698971).Curated
Sequence conflicti1234 – 12341M → I in AAY89336 (PubMed:17698971).Curated
Sequence conflicti1234 – 12341M → I in AAI63563 (PubMed:23594743).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY523999 mRNA. Translation: AAS92270.1.
DQ026829 mRNA. Translation: AAY89336.1.
BX088688, BX511058 Genomic DNA. Translation: CAM14201.1.
BX248242 Genomic DNA. Translation: CAI11526.1.
BX511058, BX088688 Genomic DNA. Translation: CAH68950.2.
BC163563 mRNA. Translation: AAI63563.1.
AY833405 mRNA. Translation: AAV93319.1.
RefSeqiNP_001019824.2. NM_001024653.2.
UniGeneiDr.91385.

Genome annotation databases

EnsembliENSDART00000049204; ENSDARP00000049203; ENSDARG00000017321.
GeneIDi554230.
KEGGidre:554230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY523999 mRNA. Translation: AAS92270.1.
DQ026829 mRNA. Translation: AAY89336.1.
BX088688, BX511058 Genomic DNA. Translation: CAM14201.1.
BX248242 Genomic DNA. Translation: CAI11526.1.
BX511058, BX088688 Genomic DNA. Translation: CAH68950.2.
BC163563 mRNA. Translation: AAI63563.1.
AY833405 mRNA. Translation: AAV93319.1.
RefSeqiNP_001019824.2. NM_001024653.2.
UniGeneiDr.91385.

3D structure databases

ProteinModelPortaliQ5GIT4.
SMRiQ5GIT4. Positions 825-1181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000049203.

Chemistry

BindingDBiQ5GIT4.
ChEMBLiCHEMBL2331049.

Proteomic databases

PaxDbiQ5GIT4.
PRIDEiQ5GIT4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000049204; ENSDARP00000049203; ENSDARG00000017321.
GeneIDi554230.
KEGGidre:554230.

Organism-specific databases

CTDi3791.
ZFINiZDB-GENE-041001-112. kdr.

Phylogenomic databases

eggNOGiKOG0200. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118923.
HOGENOMiHOG000037949.
HOVERGENiHBG053432.
InParanoidiQ5GIT4.
KOiK05098.
OMAiPSYMISY.
OrthoDBiEOG091G01TL.
PhylomeDBiQ5GIT4.
TreeFamiTF325768.

Enzyme and pathway databases

ReactomeiR-DRE-194306. Neurophilin interactions with VEGF and VEGFR.
R-DRE-195399. VEGF binds to VEGFR leading to receptor dimerization.
R-DRE-216083. Integrin cell surface interactions.
R-DRE-3928663. EPHA-mediated growth cone collapse.
R-DRE-4420097. VEGFA-VEGFR2 Pathway.
R-DRE-5218921. VEGFR2 mediated cell proliferation.

Miscellaneous databases

PROiQ5GIT4.

Gene expression databases

BgeeiENSDARG00000017321.

Family and domain databases

Gene3Di2.60.40.10. 7 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR001824. Tyr_kinase_rcpt_3_CS.
IPR009136. VEGFR2_rcpt.
IPR009137. VEGFR3_rcpt.
[Graphical view]
PANTHERiPTHR24416:SF45. PTHR24416:SF45. 3 hits.
PfamiPF07679. I-set. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR01835. VEGFRECEPTR3.
SMARTiSM00409. IG. 7 hits.
SM00408. IGc2. 4 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 8 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50835. IG_LIKE. 5 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVGFR2_DANRE
AccessioniPrimary (citable) accession number: Q5GIT4
Secondary accession number(s): B3DJQ0
, Q1ANK7, Q5MD88, Q5RIP2, Q5TZ34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: October 14, 2008
Last modified: September 7, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

This entry should not be confused with kdrl (AC Q8AXB3), which used to be called kdr.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.