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Protein

Vascular endothelial growth factor receptor 2

Gene

kdr

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. Combinations of multiple VEGF receptors are required for development of different blood vessel types in the embryo. Involved in angiogenesis, specifically in VEGF-induced sprouting of new blood vessels. Particularly involved in artery formation. Does not appear to be required for hematopoiesis.2 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei877ATPPROSITE-ProRule annotation1
Active sitei1039Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi849 – 857ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: ZFIN
  • blood vessel development Source: ZFIN
  • cell differentiation Source: UniProtKB-KW
  • cellular response to vascular endothelial growth factor stimulus Source: UniProtKB
  • vascular endothelial growth factor receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Angiogenesis, Differentiation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DRE-194306. Neurophilin interactions with VEGF and VEGFR.
R-DRE-195399. VEGF binds to VEGFR leading to receptor dimerization.
R-DRE-216083. Integrin cell surface interactions.
R-DRE-3928663. EPHA-mediated growth cone collapse.
R-DRE-4420097. VEGFA-VEGFR2 Pathway.
R-DRE-5218921. VEGFR2 mediated cell proliferation.

Names & Taxonomyi

Protein namesi
Recommended name:
Vascular endothelial growth factor receptor 2 (EC:2.7.10.1)
Short name:
VEGFR-2
Alternative name(s):
Fetal liver kinase 1b
Short name:
FLK-1b
Kinase insert domain receptor
Kinase insert domain receptor-B
Protein-tyrosine kinase receptor flk-1b
Vascular endothelial growth factor receptor 2 homolog B
Short name:
VEGFR-2 homolog B
Gene namesi
Name:kdr
Synonyms:flk1b, kdrb
ORF Names:si:busm1-205d10.1, si:ch211-254j6.1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 20

Organism-specific databases

ZFINiZDB-GENE-041001-112. kdr.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 774ExtracellularSequence analysisAdd BLAST752
Transmembranei775 – 795HelicalSequence analysisAdd BLAST21
Topological domaini796 – 1357CytoplasmicSequence analysisAdd BLAST562

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Membrane, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2331049.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000024946523 – 1357Vascular endothelial growth factor receptor 2Add BLAST1335

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...)Sequence analysis1
Glycosylationi44N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi53 ↔ 104PROSITE-ProRule annotation
Glycosylationi66N-linked (GlcNAc...)Sequence analysis1
Glycosylationi97N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi153 ↔ 203PROSITE-ProRule annotation
Glycosylationi161N-linked (GlcNAc...)Sequence analysis1
Glycosylationi209N-linked (GlcNAc...)Sequence analysis1
Glycosylationi247N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi248 ↔ 314PROSITE-ProRule annotation
Glycosylationi272N-linked (GlcNAc...)Sequence analysis1
Glycosylationi303N-linked (GlcNAc...)Sequence analysis1
Glycosylationi307N-linked (GlcNAc...)Sequence analysis1
Glycosylationi407N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi457 ↔ 538PROSITE-ProRule annotation
Glycosylationi501N-linked (GlcNAc...)Sequence analysis1
Glycosylationi560N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi579 ↔ 651PROSITE-ProRule annotation
Glycosylationi621N-linked (GlcNAc...)Sequence analysis1
Glycosylationi631N-linked (GlcNAc...)Sequence analysis1
Glycosylationi640N-linked (GlcNAc...)Sequence analysis1
Glycosylationi681N-linked (GlcNAc...)Sequence analysis1
Glycosylationi688N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi697 ↔ 746PROSITE-ProRule annotation
Glycosylationi713N-linked (GlcNAc...)Sequence analysis1
Modified residuei1065Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1070Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1186Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1222Phosphotyrosine; by autocatalysisBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ5GIT4.
PRIDEiQ5GIT4.

Expressioni

Tissue specificityi

First expressed in embryos between 5- and 7-somites in the bilateral stripes that contain the developing angioblasts, and then localized to the intermediate cell mass (ICM) and the developing vasculature. By 30 hpf, expressed in the major trunk, head and intersomitic vessels, persisting through 4 dpf when expression is seen in developing subintestinal veins and in the remaining vasculature.3 Publications

Developmental stagei

The first vegfr expressed during development.1 Publication

Gene expression databases

BgeeiENSDARG00000017321.

Interactioni

Subunit structurei

Interacts with isoform VEGF165 of vegfaa and, to a lesser extent, with isoform VEGF171 of vegfab.1 Publication

Protein-protein interaction databases

STRINGi7955.ENSDARP00000049203.

Chemistry databases

BindingDBiQ5GIT4.

Structurei

3D structure databases

ProteinModelPortaliQ5GIT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 120Ig-like C2-type 1Add BLAST89
Domaini120 – 222Ig-like C2-type 2Add BLAST103
Domaini216 – 330Ig-like C2-type 3Add BLAST115
Domaini335 – 426Ig-like C2-type 4Add BLAST92
Domaini433 – 553Ig-like C2-type 5Add BLAST121
Domaini556 – 667Ig-like C2-type 6Add BLAST112
Domaini676 – 762Ig-like C2-type 7Add BLAST87
Domaini843 – 1173Protein kinasePROSITE-ProRule annotationAdd BLAST331

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0200. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118923.
HOGENOMiHOG000037949.
HOVERGENiHBG053432.
InParanoidiQ5GIT4.
KOiK05098.
OMAiPSYMISY.
OrthoDBiEOG091G01TL.
PhylomeDBiQ5GIT4.
TreeFamiTF325768.

Family and domain databases

Gene3Di2.60.40.10. 7 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR001824. Tyr_kinase_rcpt_3_CS.
IPR009136. VEGFR2_rcpt.
IPR009137. VEGFR3_rcpt.
[Graphical view]
PANTHERiPTHR24416:SF45. PTHR24416:SF45. 3 hits.
PfamiPF07679. I-set. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR01835. VEGFRECEPTR3.
SMARTiSM00409. IG. 7 hits.
SM00408. IGc2. 4 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 8 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50835. IG_LIKE. 5 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5GIT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTSYALLL LDILLTFNVA KAIELRFVPD PPTLNITEKT IKINASDTLQ
60 70 80 90 100
ITCRGRQILE WSTPHNRTSS ETRLTISDCS GDGLFCSTLT LSKAVANETG
110 120 130 140 150
EYRCFYKSLP KEDGKTSVAV YVFIQDYRTP FVRIAQDYDV VFIREGEQVV
160 170 180 190 200
IPCLVSVEDL NVTLYTKYPV KELSTDGKEV IWDSRRGFIL PSRVVSYAGV
210 220 230 240 250
VYCQTTIRNE TFQSSPYIVA VVGYKIYDLT LSPQHERLTV GERLILNCTA
260 270 280 290 300
HTELNVGIDF QWTFPHEKRS VNGSMSTSRY KTSSNKKKLW NSLELSNTLT
310 320 330 340 350
VENVTLNDTG EYICTASSGQ MQKIAQASLI VYEKPFIALS DQLWQTVEAK
360 370 380 390 400
AGDAEAKILV KYYAYPEPAV RWYKNDQLIV LRDEYRMKFY RGVHLTIYGV
410 420 430 440 450
TEKDAGNYTV VMTNKITKEE QRRTFQLVVN DLPRIFEKDV SLDRDVHMYG
460 470 480 490 500
SSPTLTCTAS GGSSPVTIKW QWMPREDCPV RFLPKSDTRM AKCDKWREMS
510 520 530 540 550
NNTGKNPLIS QTSVDERTLK TISTLKIQKA VDHALYRCIA TNKMGQDQRV
560 570 580 590 600
IVFQVTRFLN LSVLPSSSPI EGQDVIMRCV ADRLLYYNLR WYRVANVANH
610 620 630 640 650
DPPPAAVPCD TLTLSHLHQP NVTVSGLQGT NVTLDMPIPN ATMMDQGLYA
660 670 680 690 700
CQVEIVGTNE KTCLLHNLRL RALEMSRIVT NLTDQRVNVS DSTTLVCEVS
710 720 730 740 750
GTPTPTIVWT KDNQTVMEGS GVILKRSNRV LTIQRVKKED SGLYICTACN
760 770 780 790 800
QQGCESSEAR ISVDGAEEKM NVELIMPIGA VVIAMFLWLL IVFVIRNRKR
810 820 830 840 850
PNDGDLKTGY LSIILDSDDM PMDEHCERLT YDASKWEFPR DRLKLGEPLG
860 870 880 890 900
RGAFGQVVEA TAYGIEKATT CTTVAVKMLK EGATSSEYRA LMSELKILIH
910 920 930 940 950
IGHHLNVVNL LGACTKQGGP LMVIVEYCKH GNLSSYLKSK RGEYSPYKKR
960 970 980 990 1000
TPRMPNRREV QQDEDPREGD LGLGTSTRLD ICTGTAVCTR TGEQTYKTLQ
1010 1020 1030 1040 1050
DEQESSDWDH LTMEDLISYS FQVAKGMEFL ASRKCIHRDL AARNILLSEN
1060 1070 1080 1090 1100
SVVKICDFGL ARDVYKDPDY VRKGDARLPL KWMAPETIFD RVYTTQSDVW
1110 1120 1130 1140 1150
SFGVLLWEIF SLGASPYPGV CIDESFCRRL KEGTRMRAPD YATPEIYQTM
1160 1170 1180 1190 1200
LDCWLDRPLD RPTFTQLVEH LGNLLQASAQ QDGKDYIPLT NGEMEEELVA
1210 1220 1230 1240 1250
PHLNVTSKRS FYAGNTEAQL HYDNAPPLGF PQQMNSSGVP VNMTGFVDIP
1260 1270 1280 1290 1300
LEHTTVMDGH VDCGVGLSRE QMKALDRQAQ RPLNFSPLLR CKSKESLASE
1310 1320 1330 1340 1350
SSNQTSGYQS GYHSDDAEAP IYANEEMILK RDIRKKPPLP KRNDKFSAEV

RYSAPPV
Length:1,357
Mass (Da):153,042
Last modified:October 14, 2008 - v2
Checksum:i43B97B484DBEA38F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17F → V in AAI63563 (PubMed:23594743).Curated1
Sequence conflicti72T → S in AAI63563 (PubMed:23594743).Curated1
Sequence conflicti109L → P in AAY89336 (PubMed:17698971).Curated1
Sequence conflicti254L → R in AAY89336 (PubMed:17698971).Curated1
Sequence conflicti262W → C in AAI63563 (PubMed:23594743).Curated1
Sequence conflicti287K → R in AAS92270 (Ref. 1) Curated1
Sequence conflicti384E → G in AAY89336 (PubMed:17698971).Curated1
Sequence conflicti459A → G in AAY89336 (PubMed:17698971).Curated1
Sequence conflicti488 – 489TR → PS in AAY89336 (PubMed:17698971).Curated2
Sequence conflicti488 – 489TR → PS in AAI63563 (PubMed:23594743).Curated2
Sequence conflicti553F → S in AAS92270 (Ref. 1) Curated1
Sequence conflicti958R → K in AAS92270 (Ref. 1) Curated1
Sequence conflicti996Y → S in AAY89336 (PubMed:17698971).Curated1
Sequence conflicti996Y → S in AAI63563 (PubMed:23594743).Curated1
Sequence conflicti996Y → S in AAV93319 (PubMed:16617120).Curated1
Sequence conflicti997K → T in AAY89336 (PubMed:17698971).Curated1
Sequence conflicti997K → T in AAI63563 (PubMed:23594743).Curated1
Sequence conflicti1100W → R in AAS92270 (Ref. 1) Curated1
Sequence conflicti1223D → G in AAY89336 (PubMed:17698971).Curated1
Sequence conflicti1234M → I in AAY89336 (PubMed:17698971).Curated1
Sequence conflicti1234M → I in AAI63563 (PubMed:23594743).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY523999 mRNA. Translation: AAS92270.1.
DQ026829 mRNA. Translation: AAY89336.1.
BX088688, BX511058 Genomic DNA. Translation: CAM14201.1.
BX248242 Genomic DNA. Translation: CAI11526.1.
BX511058, BX088688 Genomic DNA. Translation: CAH68950.2.
BC163563 mRNA. Translation: AAI63563.1.
AY833405 mRNA. Translation: AAV93319.1.
RefSeqiNP_001019824.2. NM_001024653.2.
UniGeneiDr.91385.

Genome annotation databases

EnsembliENSDART00000049204; ENSDARP00000049203; ENSDARG00000017321.
GeneIDi554230.
KEGGidre:554230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY523999 mRNA. Translation: AAS92270.1.
DQ026829 mRNA. Translation: AAY89336.1.
BX088688, BX511058 Genomic DNA. Translation: CAM14201.1.
BX248242 Genomic DNA. Translation: CAI11526.1.
BX511058, BX088688 Genomic DNA. Translation: CAH68950.2.
BC163563 mRNA. Translation: AAI63563.1.
AY833405 mRNA. Translation: AAV93319.1.
RefSeqiNP_001019824.2. NM_001024653.2.
UniGeneiDr.91385.

3D structure databases

ProteinModelPortaliQ5GIT4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000049203.

Chemistry databases

BindingDBiQ5GIT4.
ChEMBLiCHEMBL2331049.

Proteomic databases

PaxDbiQ5GIT4.
PRIDEiQ5GIT4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000049204; ENSDARP00000049203; ENSDARG00000017321.
GeneIDi554230.
KEGGidre:554230.

Organism-specific databases

CTDi3791.
ZFINiZDB-GENE-041001-112. kdr.

Phylogenomic databases

eggNOGiKOG0200. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118923.
HOGENOMiHOG000037949.
HOVERGENiHBG053432.
InParanoidiQ5GIT4.
KOiK05098.
OMAiPSYMISY.
OrthoDBiEOG091G01TL.
PhylomeDBiQ5GIT4.
TreeFamiTF325768.

Enzyme and pathway databases

ReactomeiR-DRE-194306. Neurophilin interactions with VEGF and VEGFR.
R-DRE-195399. VEGF binds to VEGFR leading to receptor dimerization.
R-DRE-216083. Integrin cell surface interactions.
R-DRE-3928663. EPHA-mediated growth cone collapse.
R-DRE-4420097. VEGFA-VEGFR2 Pathway.
R-DRE-5218921. VEGFR2 mediated cell proliferation.

Miscellaneous databases

PROiQ5GIT4.

Gene expression databases

BgeeiENSDARG00000017321.

Family and domain databases

Gene3Di2.60.40.10. 7 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR001824. Tyr_kinase_rcpt_3_CS.
IPR009136. VEGFR2_rcpt.
IPR009137. VEGFR3_rcpt.
[Graphical view]
PANTHERiPTHR24416:SF45. PTHR24416:SF45. 3 hits.
PfamiPF07679. I-set. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR01835. VEGFRECEPTR3.
SMARTiSM00409. IG. 7 hits.
SM00408. IGc2. 4 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 8 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50835. IG_LIKE. 5 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVGFR2_DANRE
AccessioniPrimary (citable) accession number: Q5GIT4
Secondary accession number(s): B3DJQ0
, Q1ANK7, Q5MD88, Q5RIP2, Q5TZ34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: October 14, 2008
Last modified: November 2, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

This entry should not be confused with kdrl (AC Q8AXB3), which used to be called kdr.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.