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Protein

Serine/threonine-protein kinase TNNI3K

Gene

Tnni3k

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May play a role in cardiac physiology.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei489 – 4891ATPPROSITE-ProRule annotation
Active sitei587 – 5871Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi468 – 4769ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • bundle of His cell to Purkinje myocyte communication Source: MGI
  • protein phosphorylation Source: MGI
  • regulation of cardiac conduction Source: BHF-UCL
  • regulation of cardiac muscle contraction Source: MGI
  • regulation of heart rate Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase TNNI3K (EC:2.7.11.1)
Alternative name(s):
Cardiac ankyrin repeat kinase
TNNI3-interacting kinase
Gene namesi
Name:Tnni3kImported
Synonyms:Cark
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2443276. Tnni3k.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Expressed at lower levels in the cytoplasm.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 834833Serine/threonine-protein kinase TNNI3KPRO_0000086758Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ5GIG6.
PRIDEiQ5GIG6.

PTM databases

iPTMnetiQ5GIG6.
PhosphoSiteiQ5GIG6.

Expressioni

Gene expression databases

BgeeiQ5GIG6.
CleanExiMM_TNNI3K.
GenevisibleiQ5GIG6. MM.

Interactioni

Subunit structurei

Interacts with TNNI3, ACTC, ACTA1, MYBPC3, AIP, BABP3 and HADHB.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070561.

Structurei

3D structure databases

ProteinModelPortaliQ5GIG6.
SMRiQ5GIG6. Positions 27-719.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati66 – 9631ANK 1Add
BLAST
Repeati100 – 12930ANK 2Add
BLAST
Repeati133 – 16230ANK 3Add
BLAST
Repeati166 – 19530ANK 4Add
BLAST
Repeati199 – 22931ANK 5Add
BLAST
Repeati233 – 26230ANK 6Add
BLAST
Repeati268 – 29730ANK 7Add
BLAST
Repeati303 – 33432ANK 8Add
BLAST
Repeati338 – 36730ANK 9Add
BLAST
Repeati380 – 40930ANK 10Add
BLAST
Domaini462 – 722261Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili21 – 4929Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi732 – 74514Poly-SerAdd
BLAST

Sequence similaritiesi

Contains 10 ANK repeats.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IT5N. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00840000129859.
HOGENOMiHOG000234421.
HOVERGENiHBG079513.
InParanoidiQ5GIG6.
KOiK17535.
OMAiAKGFFNI.
OrthoDBiEOG7XDBF3.
TreeFamiTF317710.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 3 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 10 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 6 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5GIG6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNYKSRPTQ TCSDEWKKKV SESYAIIIER LEDDLQIKEN EFQELRHIFG
60 70 80 90 100
SDEAFSEVSL NYRTERGLSL LHLCCACGGN KSHIRALMLK GLRPSRLTRN
110 120 130 140 150
GFPALHLAVY KDSLELITSL LHSGADVQQA GYGGLTALHI AAIAGHPEAV
160 170 180 190 200
EVLLQHGANV NVQDAVFFTP LHIAAYYGHE QVTSVLLKFG ADVNVSGEVG
210 220 230 240 250
DRPLHLASAK GFFNIVKLLV EGNKADVNAQ DNEDHVPLHF CSRFGHHNIV
260 270 280 290 300
SYLLQSDLEV QPHVINIYGD TPLHLACYNG NFEVAKEIVH VTGTESLTKE
310 320 330 340 350
NIFSETAFHS ACTYGKNIDL VKFLLDQNAV NINHRGRDGH TGLHSACYHG
360 370 380 390 400
HIRLVQFLLD NGADMNLVAC DPSRSSGEKD EQTCLMWAYE KGHDAIVTLL
410 420 430 440 450
KHYKRPQDEL PCNEYSQPGG DGSYVSVPSP LGKIKSMTKE KADVLLLRAE
460 470 480 490 500
LPSRFHLQLS EIEFHEIIGS GSFGKVYKGR CRNKIVAIKR YRANTYCSKS
510 520 530 540 550
DVDMFCREVS ILCQLNHPCV VQFVGACLDD PSQFAIVTQY ISGGSLFSLL
560 570 580 590 600
HEQKRILDLQ SKLIIAVDVA KGMEYLHSLT QPIIHRDLNS HNILLYEDGH
610 620 630 640 650
AVVADFGESR FLQSLDEDNM TKQPGNLRWM APEVFTQCTR YTIKADVFSY
660 670 680 690 700
ALCLWELLTG EIPFAHLKPA AAAADMAYHH IRPPIGYSIP KPISSLLMRG
710 720 730 740 750
WNACPEGRPE FSEVVRKLEE CLCNVELMSP ASSNSSGSLS PSSSSDCLLS
760 770 780 790 800
RGGPGRSHVA ALRSRFELEY ALNARSYTGW PQSVGTHTNP GLSLEEMNRG
810 820 830
AQYSAVDKYG YVSDPMSPMH LHSRRNSGSF EDGN
Length:834
Mass (Da):92,575
Last modified:July 27, 2011 - v4
Checksum:iAC8581BFA7B05216
GO
Isoform 2 (identifier: Q5GIG6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     670-675: AAAAAD → GKTRVL
     676-834: Missing.

Show »
Length:675
Mass (Da):75,386
Checksum:i772B85A471A60D38
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti659 – 6591T → I in AAS98608 (Ref. 1) Curated
Sequence conflicti659 – 6591T → I in AAS98609 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei670 – 6756AAAAAD → GKTRVL in isoform 2. 1 PublicationVSP_051886
Alternative sequencei676 – 834159Missing in isoform 2. 1 PublicationVSP_051887Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY526095 mRNA. Translation: AAS98608.1.
AY526096 mRNA. Translation: AAS98609.1.
AC125097 Genomic DNA. No translation available.
AC124174 Genomic DNA. No translation available.
AC144762 Genomic DNA. No translation available.
BC139368 mRNA. Translation: AAI39369.1.
BC139394 mRNA. Translation: AAI39395.1.
CCDSiCCDS17928.1. [Q5GIG6-1]
RefSeqiNP_796040.3. NM_177066.5. [Q5GIG6-1]
UniGeneiMm.210428.

Genome annotation databases

EnsembliENSMUST00000064076; ENSMUSP00000070561; ENSMUSG00000040086. [Q5GIG6-1]
ENSMUST00000143410; ENSMUSP00000122478; ENSMUSG00000040086. [Q5GIG6-2]
GeneIDi435766.
KEGGimmu:435766.
UCSCiuc008rus.2. mouse. [Q5GIG6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY526095 mRNA. Translation: AAS98608.1.
AY526096 mRNA. Translation: AAS98609.1.
AC125097 Genomic DNA. No translation available.
AC124174 Genomic DNA. No translation available.
AC144762 Genomic DNA. No translation available.
BC139368 mRNA. Translation: AAI39369.1.
BC139394 mRNA. Translation: AAI39395.1.
CCDSiCCDS17928.1. [Q5GIG6-1]
RefSeqiNP_796040.3. NM_177066.5. [Q5GIG6-1]
UniGeneiMm.210428.

3D structure databases

ProteinModelPortaliQ5GIG6.
SMRiQ5GIG6. Positions 27-719.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070561.

PTM databases

iPTMnetiQ5GIG6.
PhosphoSiteiQ5GIG6.

Proteomic databases

PaxDbiQ5GIG6.
PRIDEiQ5GIG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064076; ENSMUSP00000070561; ENSMUSG00000040086. [Q5GIG6-1]
ENSMUST00000143410; ENSMUSP00000122478; ENSMUSG00000040086. [Q5GIG6-2]
GeneIDi435766.
KEGGimmu:435766.
UCSCiuc008rus.2. mouse. [Q5GIG6-1]

Organism-specific databases

CTDi51086.
MGIiMGI:2443276. Tnni3k.

Phylogenomic databases

eggNOGiENOG410IT5N. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00840000129859.
HOGENOMiHOG000234421.
HOVERGENiHBG079513.
InParanoidiQ5GIG6.
KOiK17535.
OMAiAKGFFNI.
OrthoDBiEOG7XDBF3.
TreeFamiTF317710.

Miscellaneous databases

NextBioi410271.
PROiQ5GIG6.
SOURCEiSearch...

Gene expression databases

BgeeiQ5GIG6.
CleanExiMM_TNNI3K.
GenevisibleiQ5GIG6. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 3 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 10 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 6 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Chen C., Zhen Y., Liu Y., Xun L., Chen J., Zhang L., Hui R.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: KMImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiTNI3K_MOUSE
AccessioniPrimary (citable) accession number: Q5GIG6
Secondary accession number(s): B2RTJ7, Q5GIG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 108 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.