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Protein

Serine/threonine-protein kinase TNNI3K

Gene

Tnni3k

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May play a role in cardiac physiology.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei489ATPPROSITE-ProRule annotation1
Active sitei587Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi468 – 476ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • bundle of His cell to Purkinje myocyte communication Source: MGI
  • protein phosphorylation Source: MGI
  • regulation of cardiac conduction Source: BHF-UCL
  • regulation of cardiac muscle contraction Source: MGI
  • regulation of heart rate Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase TNNI3K (EC:2.7.11.1)
Alternative name(s):
Cardiac ankyrin repeat kinase
TNNI3-interacting kinase
Gene namesi
Name:Tnni3kImported
Synonyms:Cark
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2443276. Tnni3k.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Expressed at lower levels in the cytoplasm.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00000867582 – 834Serine/threonine-protein kinase TNNI3KAdd BLAST833

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ5GIG6.
PRIDEiQ5GIG6.

PTM databases

iPTMnetiQ5GIG6.
PhosphoSitePlusiQ5GIG6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040086.
CleanExiMM_TNNI3K.
GenevisibleiQ5GIG6. MM.

Interactioni

Subunit structurei

Interacts with TNNI3, ACTC, ACTA1, MYBPC3, AIP, BABP3 and HADHB.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070561.

Structurei

3D structure databases

ProteinModelPortaliQ5GIG6.
SMRiQ5GIG6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati66 – 96ANK 1Add BLAST31
Repeati100 – 129ANK 2Add BLAST30
Repeati133 – 162ANK 3Add BLAST30
Repeati166 – 195ANK 4Add BLAST30
Repeati199 – 229ANK 5Add BLAST31
Repeati233 – 262ANK 6Add BLAST30
Repeati268 – 297ANK 7Add BLAST30
Repeati303 – 334ANK 8Add BLAST32
Repeati338 – 367ANK 9Add BLAST30
Repeati380 – 409ANK 10Add BLAST30
Domaini462 – 722Protein kinasePROSITE-ProRule annotationAdd BLAST261

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili21 – 49Sequence analysisAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi732 – 745Poly-SerAdd BLAST14

Sequence similaritiesi

Contains 10 ANK repeats.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IT5N. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00860000133794.
HOGENOMiHOG000234421.
HOVERGENiHBG079513.
InParanoidiQ5GIG6.
KOiK17535.
OMAiSDPMSTM.
OrthoDBiEOG091G01QL.
TreeFamiTF317710.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 3 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 10 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 6 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5GIG6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNYKSRPTQ TCSDEWKKKV SESYAIIIER LEDDLQIKEN EFQELRHIFG
60 70 80 90 100
SDEAFSEVSL NYRTERGLSL LHLCCACGGN KSHIRALMLK GLRPSRLTRN
110 120 130 140 150
GFPALHLAVY KDSLELITSL LHSGADVQQA GYGGLTALHI AAIAGHPEAV
160 170 180 190 200
EVLLQHGANV NVQDAVFFTP LHIAAYYGHE QVTSVLLKFG ADVNVSGEVG
210 220 230 240 250
DRPLHLASAK GFFNIVKLLV EGNKADVNAQ DNEDHVPLHF CSRFGHHNIV
260 270 280 290 300
SYLLQSDLEV QPHVINIYGD TPLHLACYNG NFEVAKEIVH VTGTESLTKE
310 320 330 340 350
NIFSETAFHS ACTYGKNIDL VKFLLDQNAV NINHRGRDGH TGLHSACYHG
360 370 380 390 400
HIRLVQFLLD NGADMNLVAC DPSRSSGEKD EQTCLMWAYE KGHDAIVTLL
410 420 430 440 450
KHYKRPQDEL PCNEYSQPGG DGSYVSVPSP LGKIKSMTKE KADVLLLRAE
460 470 480 490 500
LPSRFHLQLS EIEFHEIIGS GSFGKVYKGR CRNKIVAIKR YRANTYCSKS
510 520 530 540 550
DVDMFCREVS ILCQLNHPCV VQFVGACLDD PSQFAIVTQY ISGGSLFSLL
560 570 580 590 600
HEQKRILDLQ SKLIIAVDVA KGMEYLHSLT QPIIHRDLNS HNILLYEDGH
610 620 630 640 650
AVVADFGESR FLQSLDEDNM TKQPGNLRWM APEVFTQCTR YTIKADVFSY
660 670 680 690 700
ALCLWELLTG EIPFAHLKPA AAAADMAYHH IRPPIGYSIP KPISSLLMRG
710 720 730 740 750
WNACPEGRPE FSEVVRKLEE CLCNVELMSP ASSNSSGSLS PSSSSDCLLS
760 770 780 790 800
RGGPGRSHVA ALRSRFELEY ALNARSYTGW PQSVGTHTNP GLSLEEMNRG
810 820 830
AQYSAVDKYG YVSDPMSPMH LHSRRNSGSF EDGN
Length:834
Mass (Da):92,575
Last modified:July 27, 2011 - v4
Checksum:iAC8581BFA7B05216
GO
Isoform 2 (identifier: Q5GIG6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     670-675: AAAAAD → GKTRVL
     676-834: Missing.

Show »
Length:675
Mass (Da):75,386
Checksum:i772B85A471A60D38
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti659T → I in AAS98608 (Ref. 1) Curated1
Sequence conflicti659T → I in AAS98609 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_051886670 – 675AAAAAD → GKTRVL in isoform 2. 1 Publication6
Alternative sequenceiVSP_051887676 – 834Missing in isoform 2. 1 PublicationAdd BLAST159

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY526095 mRNA. Translation: AAS98608.1.
AY526096 mRNA. Translation: AAS98609.1.
AC125097 Genomic DNA. No translation available.
AC124174 Genomic DNA. No translation available.
AC144762 Genomic DNA. No translation available.
BC139368 mRNA. Translation: AAI39369.1.
BC139394 mRNA. Translation: AAI39395.1.
CCDSiCCDS17928.1. [Q5GIG6-1]
RefSeqiNP_796040.3. NM_177066.5. [Q5GIG6-1]
UniGeneiMm.210428.

Genome annotation databases

EnsembliENSMUST00000064076; ENSMUSP00000070561; ENSMUSG00000040086. [Q5GIG6-1]
ENSMUST00000143410; ENSMUSP00000122478; ENSMUSG00000040086. [Q5GIG6-2]
GeneIDi435766.
KEGGimmu:435766.
UCSCiuc008rus.2. mouse. [Q5GIG6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY526095 mRNA. Translation: AAS98608.1.
AY526096 mRNA. Translation: AAS98609.1.
AC125097 Genomic DNA. No translation available.
AC124174 Genomic DNA. No translation available.
AC144762 Genomic DNA. No translation available.
BC139368 mRNA. Translation: AAI39369.1.
BC139394 mRNA. Translation: AAI39395.1.
CCDSiCCDS17928.1. [Q5GIG6-1]
RefSeqiNP_796040.3. NM_177066.5. [Q5GIG6-1]
UniGeneiMm.210428.

3D structure databases

ProteinModelPortaliQ5GIG6.
SMRiQ5GIG6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070561.

PTM databases

iPTMnetiQ5GIG6.
PhosphoSitePlusiQ5GIG6.

Proteomic databases

PaxDbiQ5GIG6.
PRIDEiQ5GIG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064076; ENSMUSP00000070561; ENSMUSG00000040086. [Q5GIG6-1]
ENSMUST00000143410; ENSMUSP00000122478; ENSMUSG00000040086. [Q5GIG6-2]
GeneIDi435766.
KEGGimmu:435766.
UCSCiuc008rus.2. mouse. [Q5GIG6-1]

Organism-specific databases

CTDi51086.
MGIiMGI:2443276. Tnni3k.

Phylogenomic databases

eggNOGiENOG410IT5N. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00860000133794.
HOGENOMiHOG000234421.
HOVERGENiHBG079513.
InParanoidiQ5GIG6.
KOiK17535.
OMAiSDPMSTM.
OrthoDBiEOG091G01QL.
TreeFamiTF317710.

Miscellaneous databases

PROiQ5GIG6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040086.
CleanExiMM_TNNI3K.
GenevisibleiQ5GIG6. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 3 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 10 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 6 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTNI3K_MOUSE
AccessioniPrimary (citable) accession number: Q5GIG6
Secondary accession number(s): B2RTJ7, Q5GIG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 113 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.