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Protein

Protein IMPACT

Gene

Impact

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Repressor
Biological processDifferentiation, Neurogenesis, Stress response, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein IMPACT
Alternative name(s):
Imprinted and ancient gene protein homolog
Gene namesi
Name:Impact
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi1591977. Impact.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003308531 – 317Protein IMPACTAdd BLAST317

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei295PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5GFD9.
PRIDEiQ5GFD9.

PTM databases

iPTMnetiQ5GFD9.
PhosphoSitePlusiQ5GFD9.

Expressioni

Gene expression databases

BgeeiENSRNOG00000045844.
GenevisibleiQ5GFD9. RN.

Interactioni

Subunit structurei

Interacts with GCN1; prevents the interaction of GCN1 with EIF2AK4/GCN2 and inhibits EIF2AK4/GCN2 kinase activity. Interaction with RPL39; this interaction occurs in a GCN1-independent manner. Associates with ribosomes; this interaction occurs in a GCN1-independent manner. Associates with actin; this interaction occurs in a GCN1-independent manner.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064200.

Structurei

3D structure databases

ProteinModelPortaliQ5GFD9.
SMRiQ5GFD9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 116RWDPROSITE-ProRule annotationAdd BLAST103

Sequence similaritiesi

Belongs to the IMPACT family.Curated

Phylogenomic databases

eggNOGiKOG3299. Eukaryota.
COG1739. LUCA.
GeneTreeiENSGT00390000017571.
HOVERGENiHBG108005.
InParanoidiQ5GFD9.
OMAiIFCIKIS.
OrthoDBiEOG091G0J0V.
PhylomeDBiQ5GFD9.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
3.30.230.30. 1 hit.
InterProiView protein in InterPro
IPR023582. Impact.
IPR001498. Impact_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR006575. RWD-domain.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR020569. UPF0029_Impact_CS.
PANTHERiPTHR16301. PTHR16301. 1 hit.
PfamiView protein in Pfam
PF05773. RWD. 1 hit.
PF01205. UPF0029. 1 hit.
SMARTiView protein in SMART
SM00591. RWD. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS50908. RWD. 1 hit.
PS00910. UPF0029. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5GFD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEVESGSNQ RQNEEIEAMA AIYGEEWCVI DEVAKIFCIR ITDDMDDPKW
60 70 80 90 100
TLCLQVMLPS EYPGTAPPVY QLNAPWLKGQ ERAELAKSLE EIYMKNIGES
110 120 130 140 150
ILYQWVEKIR DALIQKSQIT EPGPDEKKKT EEEEVEGEDD PILEHPPENP
160 170 180 190 200
VKTWDLKISE SAPEAEELPP IAHGAPITDR RSTFQAHLAP VVCIDQVKKV
210 220 230 240 250
LAKLYENKKI ASATHNIYAY RIYCEDKQTF LQDSEDDGET AAGGRLLHLM
260 270 280 290 300
EILNVKNVMV VVSRWYGGIL LGPDRFKHIN NCARNILVEK NFTNSPEESA
310
KSFGKKKVKK DKKKGDH
Length:317
Mass (Da):35,995
Last modified:March 1, 2005 - v1
Checksum:i7AA2ACF198271154
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY574213 mRNA. Translation: AAS80055.1.
RefSeqiNP_001012235.1. NM_001012235.1.
UniGeneiRn.190651.

Genome annotation databases

EnsembliENSRNOT00000073771; ENSRNOP00000064200; ENSRNOG00000045844.
GeneIDi497198.
KEGGirno:497198.

Similar proteinsi

Entry informationi

Entry nameiIMPCT_RAT
AccessioniPrimary (citable) accession number: Q5GFD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2005
Last modified: May 10, 2017
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families