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Protein

Glycoprotein endo-alpha-1,2-mannosidase

Gene

Manea

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of the terminal alpha-D-glucosyl-(1,3)-D-mannosyl unit from the GlcMan9(GlcNAc)2 oligosaccharide component of the glycoprotein produced in the Golgi membrane.1 Publication

GO - Molecular functioni

  • alpha-mannosidase activity Source: RGD
  • glycoprotein endo-alpha-1,2-mannosidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.130. 5301.
ReactomeiR-RNO-964739. N-glycan trimming and elongation in the cis-Golgi.

Protein family/group databases

CAZyiGH99. Glycoside Hydrolase Family 99.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycoprotein endo-alpha-1,2-mannosidase (EC:3.2.1.130)
Short name:
Endo-alpha mannosidase
Short name:
Endomannosidase
Short name:
rEndo
Alternative name(s):
Endo-alpha-D-mannosidase
Short name:
Enman
Gene namesi
Name:Manea
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi620327. Manea.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence analysis
Transmembranei10 – 3021Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini31 – 462432LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Glycoprotein endo-alpha-1,2-mannosidasePRO_0000282318Add
BLAST

Post-translational modificationi

Undergoes proteolytic cleavage in the C-terminal region.

Proteomic databases

PaxDbiQ5GF25.
PRIDEiQ5GF25.

Expressioni

Tissue specificityi

Highly expressed in the liver and kidney.1 Publication

Gene expression databases

GenevisibleiQ5GF25. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4566608.
STRINGi10116.ENSRNOP00000011576.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 462403CatalyticCuratedAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 99 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG22. Eukaryota.
ENOG410XT8Q. LUCA.
GeneTreeiENSGT00390000016054.
HOGENOMiHOG000223388.
HOVERGENiHBG103563.
InParanoidiQ5GF25.
KOiK15538.
OMAiIETHMKQ.
OrthoDBiEOG7NGQB6.
PhylomeDBiQ5GF25.
TreeFamiTF324051.

Family and domain databases

InterProiIPR026071. Glyco_Hydrolase_99.
[Graphical view]
PANTHERiPTHR13572. PTHR13572. 1 hit.
PfamiPF16317. Glyco_hydro_99. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5GF25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKFRRRTCI ILSLFIVFIF SLMMGLKMLW PNAASFGPPF GLDLLPELRP
60 70 80 90 100
PNTHLENKAD FQRSDRIDME TNTKDLKGAG VTVHPPRASE VNLEELPPLN
110 120 130 140 150
YFVHAFYYSW YGNPQFDGKY VHWNHPVLEH WDPRIAKNYP QGRHSPPDDI
160 170 180 190 200
GSSFYPELGS YSSRDPSVIE THMKQMRSAS IGVLALSWYP PDASDENGEA
210 220 230 240 250
TDYLVPTILD KAHKYNLKVT FHIEPYSNRD DQNMHQNVKY IIDKYGNHPA
260 270 280 290 300
FYRYKTRMGH SLPMFYIYDS YITKPKTWAN LLTPSGSQSV RGSPYDGLFI
310 320 330 340 350
ALLVEEKHKY DILQSGFDGI YTYFATNGFT YGSSHQNWNK LKSFCEKNNM
360 370 380 390 400
IFIPSVGPGY IDTSIRPWNT QNTRNRINGK YYEVGLSAAL QTQPSLISIT
410 420 430 440 450
SFNEWHEGTQ IEKAVPKRTA NTVYLDYRPH KPSLYLEITR KWSEKYSKER
460
MTYALDQQLP AS
Length:462
Mass (Da):53,416
Last modified:March 1, 2005 - v1
Checksum:i0D8F1C3EB29C0785
GO

Sequence cautioni

The sequence AAB86925.1 differs from that shown.Cloning artifact. The sequence differs from that shown at the N-terminus (1-54).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023657 mRNA. Translation: AAB86925.1. Sequence problems.
AY599499 mRNA. Translation: AAT44962.1.
RefSeqiNP_542963.2. NM_080785.2.
XP_006238022.1. XM_006237960.1.
XP_006238023.1. XM_006237961.2.
UniGeneiRn.10855.

Genome annotation databases

EnsembliENSRNOT00000011576; ENSRNOP00000011576; ENSRNOG00000008626.
GeneIDi140808.
KEGGirno:140808.
UCSCiRGD:620327. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023657 mRNA. Translation: AAB86925.1. Sequence problems.
AY599499 mRNA. Translation: AAT44962.1.
RefSeqiNP_542963.2. NM_080785.2.
XP_006238022.1. XM_006237960.1.
XP_006238023.1. XM_006237961.2.
UniGeneiRn.10855.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4566608.
STRINGi10116.ENSRNOP00000011576.

Protein family/group databases

CAZyiGH99. Glycoside Hydrolase Family 99.

Proteomic databases

PaxDbiQ5GF25.
PRIDEiQ5GF25.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011576; ENSRNOP00000011576; ENSRNOG00000008626.
GeneIDi140808.
KEGGirno:140808.
UCSCiRGD:620327. rat.

Organism-specific databases

CTDi79694.
RGDi620327. Manea.

Phylogenomic databases

eggNOGiENOG410IG22. Eukaryota.
ENOG410XT8Q. LUCA.
GeneTreeiENSGT00390000016054.
HOGENOMiHOG000223388.
HOVERGENiHBG103563.
InParanoidiQ5GF25.
KOiK15538.
OMAiIETHMKQ.
OrthoDBiEOG7NGQB6.
PhylomeDBiQ5GF25.
TreeFamiTF324051.

Enzyme and pathway databases

BRENDAi3.2.1.130. 5301.
ReactomeiR-RNO-964739. N-glycan trimming and elongation in the cis-Golgi.

Miscellaneous databases

NextBioi620723.
PROiQ5GF25.

Gene expression databases

GenevisibleiQ5GF25. RN.

Family and domain databases

InterProiIPR026071. Glyco_Hydrolase_99.
[Graphical view]
PANTHERiPTHR13572. PTHR13572. 1 hit.
PfamiPF16317. Glyco_hydro_99. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and expression of rat liver endo-alpha-mannosidase, an N-linked oligosaccharide processing enzyme."
    Spiro M.J., Bhoyroo V.D., Spiro R.G.
    J. Biol. Chem. 272:29356-29363(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 245-253; 277-291 AND 381-400, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, PTM, SUBCELLULAR LOCATION, TOPOLOGY.
    Strain: Sprague-Dawley.
    Tissue: Liver.

Entry informationi

Entry nameiMANEA_RAT
AccessioniPrimary (citable) accession number: Q5GF25
Secondary accession number(s): O35390
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 1, 2005
Last modified: November 11, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.