ID   L_TAVCV                 Reviewed;        1928 AA.
AC   Q5GA85;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   16-JUN-2009, entry version 21.
DE   RecName: Full=Large structural protein;
DE            Short=Protein L;
DE   AltName: Full=Transcriptase;
DE   AltName: Full=Replicase;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=mRNA (guanine-N(7)-)-methyltransferase;
DE              EC=2.1.1.56;
DE   Includes:
DE     RecName: Full=mRNA guanylyltransferase;
DE              EC=2.7.7.-;
GN   Name=L;
OS   Taro vein chlorosis virus (TAVCV).
OC   Viruses; ssRNA negative-strand viruses; Mononegavirales;
OC   Rhabdoviridae; Nucleorhabdovirus; unclassified Nucleorhabdovirus.
OX   NCBI_TaxID=303300;
OH   NCBI_TaxID=4460; Colocasia esculenta (Elephant's ear) (Taro).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=15659770; DOI=10.1099/vir.0.80591-0;
RA   Revill P., Trinh X., Dale J., Harding R.;
RT   "Taro vein chlorosis virus: characterization and variability of a new
RT   nucleorhabdovirus.";
RL   J. Gen. Virol. 86:491-499(2005).
CC   -!- FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl
CC       transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A)
CC       synthetase activities. The viral mRNA guanylyl transferase
CC       displays a different biochemical reaction than the cellular
CC       enzyme. The template is composed of the viral RNA tightly
CC       encapsidated by the nucleoprotein (N). Functions either as
CC       transcriptase or as replicase. The transcriptase synthesizes
CC       subsequently five subgenomic RNAs, assuring their capping and
CC       polyadenylation by a stuttering mechanism. The replicase mode is
CC       dependent on intracellular N protein concentration. In this mode,
CC       the polymerase replicates the whole viral genome without
CC       recognizing the transcriptional signals (By similarity).
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-
CC       adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
CC   -!- SUBUNIT: Interacts with the P protein (By similarity).
CC   -!- SUBCELLULAR LOCATION: Virion (Potential). Host cytoplasm (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the rhabdoviridae protein L family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AY674964; AAV92087.1; -; Genomic_RNA.
DR   RefSeq; YP_224083.1; -.
DR   GeneID; 5076497; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006370; P:mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR014023; RNA_pol_cat.
DR   InterPro; IPR001016; RNA_pol_L_viral.
DR   Pfam; PF00946; Paramyx_RNA_pol; 1.
DR   PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Methyltransferase; mRNA capping;
KW   mRNA processing; Multifunctional enzyme; Nucleotide-binding;
KW   Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase;
KW   S-adenosyl-L-methionine; Transferase; Virion.
FT   CHAIN         1   1928       Large structural protein.
FT                                /FTId=PRO_0000297841.
FT   DOMAIN      596    782       RdRp catalytic.
SQ   SEQUENCE   1928 AA;  217363 MW;  042E4E6FBC396429 CRC64;
     MDSDYPDLDP EALTVLDSIR EGIQDEEEED NNDKILSGTG DYHLKSALRT LDDMTRHPIF
     NKEYQKAVRH FGISPTMMMT PTAVLKLTVS QTKINKAVGF LFGDILVRLD SLPWAVDCYD
     SIQAEIKTMH SHMMIFATPS WVEDVHNKVS SLVEYDHDAT LIWATVITLK NYLPAWREKG
     ASLLDWRSVQ YDPESEYLVM KVDRDFIIYV GSDICVMEIG KQTLWAPVPY ILNGADKVAE
     RYNVKYYCAL CDELDIPDRI SLEKLNQIIE VGDDCLQALG NKGYDIIGSY EALLAGIIQA
     RDNPQVIPDR ELLQRTTLND PGNTIGVTFL KRWDALMEDL NPEQIACAHG LYRIWGHPAV
     DILGGINKMR EVASIVKLPS SKILTDIGRQ FKEMFFTSYH SVHKHYPKHL IREYKSDSYI
     HECLKDNRTL NSKVLSYHFP DWDSVALEKN FEVPYSWNLV HNLKDKAISP SRSELYETLS
     TRNSIFGASN RRGILKSLTM ETVQLRAFLQ DVNDKGLPDN DKIIGVYPKE RELKIKARLF
     SLMSFKLRLY FVSTEALLGD KILKYFPQIT MSLDMLSMIK KMFRVSGQTT RGDDSVTVIF
     NLDFVKWNLQ MRKIICSPVF TQLGALFGMP NLFDITHDLF RESVIYLCSG EGDLRGDPVF
     GVAPDGVWSW TGDESGKEGL RQKGWTILTV VTIMLIAKRH HVDVSLMGGG DNQVLGITIG
     GMVRDSVGEL TQDSCKLAQC TIKRFTQDLI TTFGDLGLPL KASETWVSDS LFMYNKHMFY
     KGMPLRSPLK AVSRIFPLAN DSIMTLDNMI NNISSGVKAA CMKERHGIPL VFIKTMAYRR
     VAELSLIMHP LTVCFKKPEL PDHGIVARSG KKLKIPVTSK NLRQYFSLCT LGSSTMGHPG
     TLHLPDIIMR GFPDPLTSHL SFISEMRRYI VDPGLASVVD KLSHLSTSPT TEYAKLVEDP
     TSINHDAPTH GLNEIRQMSR DFLMSTTLAT NPHLKSLFSL LDRGTEKDFY DALCSAQELD
     VKVLHEIAGA TLYGYTNGIA SRIDQTGTVR ALNENIDVLR RLALAETRYI GYLMARDTRE
     HDLKPSSCSR ITAQQYRDLS WRKPILGVTV PHPMEMCQIM SSTETIYHDA VVCWSDRVSG
     SEIYQSMGQG KIYQGSYTKE RFKATDIAAA YGNEDILVKA VRLQKLINWR YDEGSNFAKI
     ISLTLEAITD ANTEGFHRSK EEIKGEFDHR RGVTGDISGG IPNFLVTPTS HFSSTTSSWV
     SHSRGGKNEN IHFQSVLINL LYRAMVYRGS VPGLPEMIWY SKEKCSDCIT EIKDPDPIKT
     TPSLHTLPSA KGNPFAYIES VNVKLDYHHQ IEITKGMEEE YLINSLWDGQ NVSGEEESGL
     LLYLMLIGSR QISESFILLM RERINAATAL QYMLNRVILA RKLGLDSQFP IRSTSCVNLL
     LGTDDNILSC RDRFNIELLS GSWESGVSCD MSVLYRDDLL TASELHVNVY LQNVPLQLAL
     SRAASSTQLQ SCLECQAIVL DRPSQRELMR YLHWCCPYHT ANAPPRILRI HSEKLIKGIE
     LRTDNPLLVP YVCNPVTLER VEKVPVMVDS WELPVHMHST WDSILPQLYL TLKSLLSQVT
     ISSLIVDDDI TLINLAASVM LDLRRELPVY INVKGFSGTE INNKFDNLKL LPPNIRSSVS
     VYHENRSLIE ASAAVWLPEP SSVESVGADW LVLWGDTWRM GAGVPGHVLV TESKLSTGMA
     WVLHRDPLAS QSVLELCGAV QIWEKKALDW DMREGHCVPI QMNRTLACSR LGSRGVRWTM
     WSTAAGLDKV TRILRSKLLS LSGNPGSSYH WRKGCQKILK AYVLSLYAHC VDNMLEASGR
     LVGVSVHGSL SGIVPICDMH SSDRIQRAQY LFLKERCQGG PFILRNRLER RINLLSPVHD
     LLDGPSQS
//