Reviewed, UniProtKB/Swiss-Prot Q5GA85 (L_TAVCV)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Taro vein chlorosis virus (TAVCV)

Taxonomic identifier

303300 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Nucleorhabdovirus › unclassified Nucleorhabdovirus

Virus host

Colocasia esculenta (Elephant's ear) (Taro) [TaxID: 4460]

Protein attributes

Sequence length	1928 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	VirionPotential. CytoplasmBy similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW virion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1928	1928	Large structural protein		PRO_0000297841
Regions
	Domain	596 – 782	187	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q5GA85-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 042E4E6FBC396429	FASTA	1,928	217,363
	10 20 30 40 50 60 MDSDYPDLDP EALTVLDSIR EGIQDEEEED NNDKILSGTG DYHLKSALRT LDDMTRHPIF 70 80 90 100 110 120 NKEYQKAVRH FGISPTMMMT PTAVLKLTVS QTKINKAVGF LFGDILVRLD SLPWAVDCYD 130 140 150 160 170 180 SIQAEIKTMH SHMMIFATPS WVEDVHNKVS SLVEYDHDAT LIWATVITLK NYLPAWREKG 190 200 210 220 230 240 ASLLDWRSVQ YDPESEYLVM KVDRDFIIYV GSDICVMEIG KQTLWAPVPY ILNGADKVAE 250 260 270 280 290 300 RYNVKYYCAL CDELDIPDRI SLEKLNQIIE VGDDCLQALG NKGYDIIGSY EALLAGIIQA 310 320 330 340 350 360 RDNPQVIPDR ELLQRTTLND PGNTIGVTFL KRWDALMEDL NPEQIACAHG LYRIWGHPAV 370 380 390 400 410 420 DILGGINKMR EVASIVKLPS SKILTDIGRQ FKEMFFTSYH SVHKHYPKHL IREYKSDSYI 430 440 450 460 470 480 HECLKDNRTL NSKVLSYHFP DWDSVALEKN FEVPYSWNLV HNLKDKAISP SRSELYETLS 490 500 510 520 530 540 TRNSIFGASN RRGILKSLTM ETVQLRAFLQ DVNDKGLPDN DKIIGVYPKE RELKIKARLF 550 560 570 580 590 600 SLMSFKLRLY FVSTEALLGD KILKYFPQIT MSLDMLSMIK KMFRVSGQTT RGDDSVTVIF 610 620 630 640 650 660 NLDFVKWNLQ MRKIICSPVF TQLGALFGMP NLFDITHDLF RESVIYLCSG EGDLRGDPVF 670 680 690 700 710 720 GVAPDGVWSW TGDESGKEGL RQKGWTILTV VTIMLIAKRH HVDVSLMGGG DNQVLGITIG 730 740 750 760 770 780 GMVRDSVGEL TQDSCKLAQC TIKRFTQDLI TTFGDLGLPL KASETWVSDS LFMYNKHMFY 790 800 810 820 830 840 KGMPLRSPLK AVSRIFPLAN DSIMTLDNMI NNISSGVKAA CMKERHGIPL VFIKTMAYRR 850 860 870 880 890 900 VAELSLIMHP LTVCFKKPEL PDHGIVARSG KKLKIPVTSK NLRQYFSLCT LGSSTMGHPG 910 920 930 940 950 960 TLHLPDIIMR GFPDPLTSHL SFISEMRRYI VDPGLASVVD KLSHLSTSPT TEYAKLVEDP 970 980 990 1000 1010 1020 TSINHDAPTH GLNEIRQMSR DFLMSTTLAT NPHLKSLFSL LDRGTEKDFY DALCSAQELD 1030 1040 1050 1060 1070 1080 VKVLHEIAGA TLYGYTNGIA SRIDQTGTVR ALNENIDVLR RLALAETRYI GYLMARDTRE 1090 1100 1110 1120 1130 1140 HDLKPSSCSR ITAQQYRDLS WRKPILGVTV PHPMEMCQIM SSTETIYHDA VVCWSDRVSG 1150 1160 1170 1180 1190 1200 SEIYQSMGQG KIYQGSYTKE RFKATDIAAA YGNEDILVKA VRLQKLINWR YDEGSNFAKI 1210 1220 1230 1240 1250 1260 ISLTLEAITD ANTEGFHRSK EEIKGEFDHR RGVTGDISGG IPNFLVTPTS HFSSTTSSWV 1270 1280 1290 1300 1310 1320 SHSRGGKNEN IHFQSVLINL LYRAMVYRGS VPGLPEMIWY SKEKCSDCIT EIKDPDPIKT 1330 1340 1350 1360 1370 1380 TPSLHTLPSA KGNPFAYIES VNVKLDYHHQ IEITKGMEEE YLINSLWDGQ NVSGEEESGL 1390 1400 1410 1420 1430 1440 LLYLMLIGSR QISESFILLM RERINAATAL QYMLNRVILA RKLGLDSQFP IRSTSCVNLL 1450 1460 1470 1480 1490 1500 LGTDDNILSC RDRFNIELLS GSWESGVSCD MSVLYRDDLL TASELHVNVY LQNVPLQLAL 1510 1520 1530 1540 1550 1560 SRAASSTQLQ SCLECQAIVL DRPSQRELMR YLHWCCPYHT ANAPPRILRI HSEKLIKGIE 1570 1580 1590 1600 1610 1620 LRTDNPLLVP YVCNPVTLER VEKVPVMVDS WELPVHMHST WDSILPQLYL TLKSLLSQVT 1630 1640 1650 1660 1670 1680 ISSLIVDDDI TLINLAASVM LDLRRELPVY INVKGFSGTE INNKFDNLKL LPPNIRSSVS 1690 1700 1710 1720 1730 1740 VYHENRSLIE ASAAVWLPEP SSVESVGADW LVLWGDTWRM GAGVPGHVLV TESKLSTGMA 1750 1760 1770 1780 1790 1800 WVLHRDPLAS QSVLELCGAV QIWEKKALDW DMREGHCVPI QMNRTLACSR LGSRGVRWTM 1810 1820 1830 1840 1850 1860 WSTAAGLDKV TRILRSKLLS LSGNPGSSYH WRKGCQKILK AYVLSLYAHC VDNMLEASGR 1870 1880 1890 1900 1910 1920 LVGVSVHGSL SGIVPICDMH SSDRIQRAQY LFLKERCQGG PFILRNRLER RINLLSPVHD LLDGPSQS

References

[1]	"Taro vein chlorosis virus: characterization and variability of a new nucleorhabdovirus." Revill P., Trinh X., Dale J., Harding R. J. Gen. Virol. 86:491-499(2005) [PubMed: 15659770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY674964 Genomic RNA. Translation: AAV92087.1.
RefSeq	YP_224083.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5076497.
Family and domain databases
InterPro	IPR014023. RNA_pol_cat. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_TAVCV

Accession

Primary (citable) accession number: Q5GA85

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	March 1, 2005
Last modified:	November 25, 2008
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)