Reviewed, UniProtKB/Swiss-Prot Q5G5B9 (GPD_MAGGR)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+]
    EC=1.1.1.8

Gene names

ORF Names:

MGG_00067

Organism

Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) [Complete proteome]

Taxonomic identifier

148305 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Glycerol-3-phosphate dehydrogenase [NAD+]		PRO_0000138091
Regions
	Nucleotide binding	17 – 22	6	NAD By similarity
	Region	349 – 350	2	Substrate binding By similarity
Sites
	Active site	283	1	Proton acceptor By similarity
	Binding site	117	1	NAD By similarity
	Binding site	140	1	NAD; via amide nitrogen By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	173	1	NAD; via amide nitrogen By similarity
	Binding site	349	1	NAD By similarity
	Binding site	378	1	NAD By similarity
Experimental info
	Sequence conflict	254	1	G → E in AAW69311. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5G5B9-1 [UniParc]. Last modified June 12, 2007. Version 2. Checksum: AAFF086871BA1913	FASTA	433	46,756
	10 20 30 40 50 60 MASLGSYAKK HKVTIIGSGN WGSTIAKIVA ESTREHKDVF EEDVQMWVFE EKVTIPKDSP 70 80 90 100 110 120 YYESEEPQKL TEVINKHHEN VKYLPGIKLP SNIIANPSLT DAVRDSSVLV FNLPHEFLGK 130 140 150 160 170 180 VCQQLNGHIV PFARGISCIK GVDVSGSGIN LFCEVIGEKL GIYCGALSGA NVASQIAAEE 190 200 210 220 230 240 GVSETTIAYD PPPIDSSRAA TPRDRSPNYD STSANKLPDL TVTSADSNGK DDRGRRTKAK 250 260 270 280 290 300 LTPVPESYPP LDHGTLQILF DRPYFSVSMV SDVAGVSLSG ALKNIVALAA GFVDGKGWGS 310 320 330 340 350 360 NVQSAVIRVG LAEMLKFARE FFGESVDPFT ILLESAGVAD VITSCISGRN FRCASMAVKR 370 380 390 400 410 420 GVSVAEIEEK ELNGQKLQGT STAKEVNSLL KARGREGDYP LFTTVNEILE GKARVDDLPK 430 LVIRQKHTIE KSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Chen B.S., Li Y.Z., Peng Y.L., Dong H.T., Li D.B. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Y34.
[2]	"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed: 15846337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / FGSC 8958.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY850305 mRNA. Translation: AAW69311.1. CH476836 Genomic DNA. Translation: EDK03378.1.
RefSeq	XP_369177.1.
UniGene	Mgr.3617
3D structure databases
SMR	Q5G5B9. Positions 11-424.
ModBase	Search...
Genome annotation databases
GeneID	2675060.
KEGG	mgr:MGG_00067.
NMPDR	fig|242507.1.peg.1094.
Phylogenomic databases
OMA	LPHQFIG.
OrthoDB	EOG9J9PGK.
PhylomeDB	Q5G5B9.
Enzyme and pathway databases
BRENDA	1.1.1.8. 5953.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR016040. NAD(P)-bd_dom. IPR006168. NAD-dep_Gly3P_DH. IPR017751. NAD-dep_Gly3P_DH_euk. IPR011128. NAD-dep_Gly3P_DH_N. IPR006109. NAD_Gly3P_DH_C. [Graphical view]
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PRINTS	PR00077. GPDHDRGNASE.
TIGRFAMs	TIGR03376. glycerol3P_DH. 1 hit.
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPD_MAGGR

Accession

Primary (citable) accession number: Q5G5B9
Secondary accession number(s): A4RGF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	June 12, 2007
Last modified:	February 9, 2010
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)