Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q5FYB1

- ARSI_HUMAN

UniProt

Q5FYB1 - ARSI_HUMAN

Protein

Arylsulfatase I

Gene

ARSI

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Displays arylsulfatase activity at neutral pH, when co-expressed with SUMF1; arylsulfatase activity is measured in the secretion medium of retinal cell line, but no activity is recorded when measured in cell extracts.2 Publications

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi55 – 551CalciumBy similarity
    Metal bindingi56 – 561CalciumBy similarity
    Metal bindingi93 – 931Calcium; via 3-oxoalanineBy similarity
    Binding sitei147 – 1471SubstrateBy similarity
    Active sitei149 – 1491By similarity
    Binding sitei239 – 2391SubstrateBy similarity
    Metal bindingi297 – 2971CalciumBy similarity
    Metal bindingi298 – 2981CalciumBy similarity
    Binding sitei315 – 3151SubstrateBy similarity

    GO - Molecular functioni

    1. arylsulfatase activity Source: HGNC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular protein metabolic process Source: Reactome
    2. glycosphingolipid metabolic process Source: Reactome
    3. post-translational protein modification Source: Reactome
    4. small molecule metabolic process Source: Reactome
    5. sphingolipid metabolic process Source: Reactome

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_116105. Glycosphingolipid metabolism.
    REACT_121036. The activation of arylsulfatases.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arylsulfatase I (EC:3.1.6.-)
    Short name:
    ASI
    Gene namesi
    Name:ARSI
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:32521. ARSI.

    Subcellular locationi

    Secreted 1 Publication. Endoplasmic reticulum 1 Publication
    Note: Localized in the intracellular granular structures.

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: Reactome
    2. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi93 – 931C → S: No arylsulfatase activity in the media of retinal epithelium cell. 1 Publication

    Organism-specific databases

    PharmGKBiPA143485309.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 569546Arylsulfatase IPRO_0000042216Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei93 – 9313-oxoalanine (Cys)
    Glycosylationi276 – 2761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi466 – 4661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi496 – 4961N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The oxidation of Cys-93 residue to 3-oxoalanine (also known as C(alpha)-formylglycine) by SUMF1/Sulfatase-modifying factor 1, seems critical for catalytic activity.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ5FYB1.
    PRIDEiQ5FYB1.

    PTM databases

    PhosphoSiteiQ5FYB1.

    Expressioni

    Tissue specificityi

    Expressed in placenta, in embryonic stem cells, fetal eyes and lens.2 Publications

    Gene expression databases

    ArrayExpressiQ5FYB1.
    BgeeiQ5FYB1.
    CleanExiHS_ARSI.
    GenevestigatoriQ5FYB1.

    Organism-specific databases

    HPAiHPA038386.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000333395.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5FYB1.
    SMRiQ5FYB1. Positions 45-523.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi527 – 5337Poly-Glu

    Sequence similaritiesi

    Belongs to the sulfatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3119.
    HOGENOMiHOG000135354.
    HOVERGENiHBG004282.
    InParanoidiQ5FYB1.
    KOiK12375.
    OMAiQRASHIL.
    OrthoDBiEOG7MKW5Q.
    PhylomeDBiQ5FYB1.
    TreeFamiTF314186.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR000917. Sulfatase.
    IPR024607. Sulfatase_CS.
    [Graphical view]
    PfamiPF00884. Sulfatase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00523. SULFATASE_1. 1 hit.
    PS00149. SULFATASE_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5FYB1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MHTLTGFSLV SLLSFGYLSW DWAKPSFVAD GPGEAGEQPS AAPPQPPHII    50
    FILTDDQGYH DVGYHGSDIE TPTLDRLAAK GVKLENYYIQ PICTPSRSQL 100
    LTGRYQIHTG LQHSIIRPQQ PNCLPLDQVT LPQKLQEAGY STHMVGKWHL 150
    GFYRKECLPT RRGFDTFLGS LTGNVDYYTY DNCDGPGVCG FDLHEGENVA 200
    WGLSGQYSTM LYAQRASHIL ASHSPQRPLF LYVAFQAVHT PLQSPREYLY 250
    RYRTMGNVAR RKYAAMVTCM DEAVRNITWA LKRYGFYNNS VIIFSSDNGG 300
    QTFSGGSNWP LRGRKGTYWE GGVRGLGFVH SPLLKRKQRT SRALMHITDW 350
    YPTLVGLAGG TTSAADGLDG YDVWPAISEG RASPRTEILH NIDPLYNHAQ 400
    HGSLEGGFGI WNTAVQAAIR VGEWKLLTGD PGYGDWIPPQ TLATFPGSWW 450
    NLERMASVRQ AVWLFNISAD PYEREDLAGQ RPDVVRTLLA RLAEYNRTAI 500
    PVRYPAENPR AHPDFNGGAW GPWASDEEEE EEEGRARSFS RGRRKKKCKI 550
    CKLRSFFRKL NTRLMSQRI 569
    Length:569
    Mass (Da):64,030
    Last modified:March 1, 2005 - v1
    Checksum:iD2F33EDD33ED211C
    GO
    Isoform 2 (identifier: Q5FYB1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-143: Missing.

    Show »
    Length:426
    Mass (Da):48,253
    Checksum:i2A12FCB6D9DCFA32
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti171 – 1711L → F in BAG53634. (PubMed:14702039)Curated
    Sequence conflicti211 – 2111L → P in BAG53634. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 143143Missing in isoform 2. 1 PublicationVSP_036022Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY875937 mRNA. Translation: AAW66665.1.
    AB448735 mRNA. Translation: BAH11166.1.
    AK122641 mRNA. Translation: BAG53634.1.
    BC129995 mRNA. Translation: AAI29996.1.
    BC129996 mRNA. Translation: AAI29997.1.
    CCDSiCCDS34275.1. [Q5FYB1-1]
    RefSeqiNP_001012301.1. NM_001012301.2. [Q5FYB1-1]
    UniGeneiHs.591252.

    Genome annotation databases

    GeneIDi340075.
    KEGGihsa:340075.
    UCSCiuc003lrv.2. human. [Q5FYB1-1]

    Polymorphism databases

    DMDMi74722581.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY875937 mRNA. Translation: AAW66665.1 .
    AB448735 mRNA. Translation: BAH11166.1 .
    AK122641 mRNA. Translation: BAG53634.1 .
    BC129995 mRNA. Translation: AAI29996.1 .
    BC129996 mRNA. Translation: AAI29997.1 .
    CCDSi CCDS34275.1. [Q5FYB1-1 ]
    RefSeqi NP_001012301.1. NM_001012301.2. [Q5FYB1-1 ]
    UniGenei Hs.591252.

    3D structure databases

    ProteinModelPortali Q5FYB1.
    SMRi Q5FYB1. Positions 45-523.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000333395.

    PTM databases

    PhosphoSitei Q5FYB1.

    Polymorphism databases

    DMDMi 74722581.

    Proteomic databases

    PaxDbi Q5FYB1.
    PRIDEi Q5FYB1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 340075.
    KEGGi hsa:340075.
    UCSCi uc003lrv.2. human. [Q5FYB1-1 ]

    Organism-specific databases

    CTDi 340075.
    GeneCardsi GC05M149657.
    HGNCi HGNC:32521. ARSI.
    HPAi HPA038386.
    MIMi 610009. gene.
    neXtProti NX_Q5FYB1.
    PharmGKBi PA143485309.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG3119.
    HOGENOMi HOG000135354.
    HOVERGENi HBG004282.
    InParanoidi Q5FYB1.
    KOi K12375.
    OMAi QRASHIL.
    OrthoDBi EOG7MKW5Q.
    PhylomeDBi Q5FYB1.
    TreeFami TF314186.

    Enzyme and pathway databases

    Reactomei REACT_116105. Glycosphingolipid metabolism.
    REACT_121036. The activation of arylsulfatases.

    Miscellaneous databases

    GenomeRNAii 340075.
    NextBioi 97681.
    PROi Q5FYB1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q5FYB1.
    Bgeei Q5FYB1.
    CleanExi HS_ARSI.
    Genevestigatori Q5FYB1.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR000917. Sulfatase.
    IPR024607. Sulfatase_CS.
    [Graphical view ]
    Pfami PF00884. Sulfatase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00523. SULFATASE_1. 1 hit.
    PS00149. SULFATASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sulfatases and sulfatase modifying factors: an exclusive and promiscuous relationship."
      Sardiello M., Annunziata I., Roma G., Ballabio A.
      Hum. Mol. Genet. 14:3203-3217(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Molecular cloning and initial characterization of three novel human sulfatases."
      Obaya A.J.
      Gene 372:110-117(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION.
    3. "Characterization of the arylsulfatase I (ARSI) gene preferentially expressed in the human retinal pigment epithelium cell line ARPE-19."
      Oshikawa M., Usami R., Kato S.
      Mol. Vis. 15:482-494(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-93, OXIDATION AT CYS-93, TISSUE SPECIFICITY.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Lung.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

    Entry informationi

    Entry nameiARSI_HUMAN
    AccessioniPrimary (citable) accession number: Q5FYB1
    Secondary accession number(s): A1L3B0, B3KV22, B7XD03
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    According to PubMed:16500042, has no arylsulfatase activity.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3