Q5FYB1 (ARSI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arylsulfatase I
Short name=ASI
EC=3.1.6.-

Gene names

Name:

ARSI

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Displays arylsulfatase activity at neutral pH, when co-expressed with SUMF1; arylsulfatase activity is measured in the secretion medium of retinal cell line, but no activity is recorded when measured in cell extracts. Ref.2 Ref.3
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted. Endoplasmic reticulum. Note: Localized in the intracellular granular structures. Ref.3
Tissue specificity	Expressed in placenta, in embryonic stem cells, fetal eyes and lens. Ref.2 Ref.3
Post-translational modification	The oxidation of Cys-93 residue to 3-oxoalanine (also known as C(alpha)-formylglycine) by SUMF1/Sulfatase-modifying factor 1, seems critical for catalytic activity.
Sequence similarities	Belongs to the sulfatase family.
Caution	According to Ref.2, has no arylsulfatase activity.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycosphingolipid metabolic process Traceable author statement. Source: Reactome post-translational protein modification Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum lumen Traceable author statement. Source: Reactome extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	arylsulfatase activity Traceable author statement Ref.1. Source: HGNC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5FYB1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5FYB1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-143: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 569	546	Arylsulfatase I		PRO_0000042216
Regions
	Compositional bias	527 – 533	7	Poly-Glu
Sites
	Active site	149	1	By similarity
	Metal binding	55	1	Calcium By similarity
	Metal binding	56	1	Calcium By similarity
	Metal binding	93	1	Calcium; via 3-oxoalanine By similarity
	Metal binding	297	1	Calcium By similarity
	Metal binding	298	1	Calcium By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	239	1	Substrate By similarity
	Binding site	315	1	Substrate By similarity
Amino acid modifications
	Modified residue	93	1	3-oxoalanine (Cys)
	Glycosylation	276	1	N-linked (GlcNAc...) Potential
	Glycosylation	288	1	N-linked (GlcNAc...) Potential
	Glycosylation	466	1	N-linked (GlcNAc...) Potential
	Glycosylation	496	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 143	143	Missing in isoform 2.		VSP_036022
Experimental info
	Mutagenesis	93	1	C → S: No arylsulfatase activity in the media of retinal epithelium cell. Ref.3
	Sequence conflict	171	1	L → F in BAG53634. Ref.4
	Sequence conflict	211	1	L → P in BAG53634. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: D2F33EDD33ED211C	FASTA	569	64,030
	10 20 30 40 50 60 MHTLTGFSLV SLLSFGYLSW DWAKPSFVAD GPGEAGEQPS AAPPQPPHII FILTDDQGYH 70 80 90 100 110 120 DVGYHGSDIE TPTLDRLAAK GVKLENYYIQ PICTPSRSQL LTGRYQIHTG LQHSIIRPQQ 130 140 150 160 170 180 PNCLPLDQVT LPQKLQEAGY STHMVGKWHL GFYRKECLPT RRGFDTFLGS LTGNVDYYTY 190 200 210 220 230 240 DNCDGPGVCG FDLHEGENVA WGLSGQYSTM LYAQRASHIL ASHSPQRPLF LYVAFQAVHT 250 260 270 280 290 300 PLQSPREYLY RYRTMGNVAR RKYAAMVTCM DEAVRNITWA LKRYGFYNNS VIIFSSDNGG 310 320 330 340 350 360 QTFSGGSNWP LRGRKGTYWE GGVRGLGFVH SPLLKRKQRT SRALMHITDW YPTLVGLAGG 370 380 390 400 410 420 TTSAADGLDG YDVWPAISEG RASPRTEILH NIDPLYNHAQ HGSLEGGFGI WNTAVQAAIR 430 440 450 460 470 480 VGEWKLLTGD PGYGDWIPPQ TLATFPGSWW NLERMASVRQ AVWLFNISAD PYEREDLAGQ 490 500 510 520 530 540 RPDVVRTLLA RLAEYNRTAI PVRYPAENPR AHPDFNGGAW GPWASDEEEE EEEGRARSFS 550 560 RGRRKKKCKI CKLRSFFRKL NTRLMSQRI
	Isoform 2 [UniParc]. Checksum: 2A12FCB6D9DCFA32	FASTA	426	48,253
	10 20 30 40 50 60 MVGKWHLGFY RKECLPTRRG FDTFLGSLTG NVDYYTYDNC DGPGVCGFDL HEGENVAWGL 70 80 90 100 110 120 SGQYSTMLYA QRASHILASH SPQRPLFLYV AFQAVHTPLQ SPREYLYRYR TMGNVARRKY 130 140 150 160 170 180 AAMVTCMDEA VRNITWALKR YGFYNNSVII FSSDNGGQTF SGGSNWPLRG RKGTYWEGGV 190 200 210 220 230 240 RGLGFVHSPL LKRKQRTSRA LMHITDWYPT LVGLAGGTTS AADGLDGYDV WPAISEGRAS 250 260 270 280 290 300 PRTEILHNID PLYNHAQHGS LEGGFGIWNT AVQAAIRVGE WKLLTGDPGY GDWIPPQTLA 310 320 330 340 350 360 TFPGSWWNLE RMASVRQAVW LFNISADPYE REDLAGQRPD VVRTLLARLA EYNRTAIPVR 370 380 390 400 410 420 YPAENPRAHP DFNGGAWGPW ASDEEEEEEE GRARSFSRGR RKKKCKICKL RSFFRKLNTR LMSQRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sulfatases and sulfatase modifying factors: an exclusive and promiscuous relationship." Sardiello M., Annunziata I., Roma G., Ballabio A. Hum. Mol. Genet. 14:3203-3217(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Molecular cloning and initial characterization of three novel human sulfatases." Obaya A.J. Gene 372:110-117(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION.
[3]	"Characterization of the arylsulfatase I (ARSI) gene preferentially expressed in the human retinal pigment epithelium cell line ARPE-19." Oshikawa M., Usami R., Kato S. Mol. Vis. 15:482-494(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-93, OXIDATION AT CYS-93, TISSUE SPECIFICITY.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY875937 mRNA. Translation: AAW66665.1. AB448735 mRNA. Translation: BAH11166.1. AK122641 mRNA. Translation: BAG53634.1. BC129995 mRNA. Translation: AAI29996.1. BC129996 mRNA. Translation: AAI29997.1.
IPI	IPI00257076. IPI00915442.
RefSeq	NP_001012301.1. NM_001012301.2.
UniGene	Hs.591252.
3D structure databases
ProteinModelPortal	Q5FYB1.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000333395.
PTM databases
PhosphoSite	Q5FYB1.
Polymorphism databases
DMDM	74722581.
Proteomic databases
PaxDb	Q5FYB1.
PRIDE	Q5FYB1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000328668; ENSP00000333395; ENSG00000183876. ENST00000515301; ENSP00000426879; ENSG00000183876.
GeneID	340075.
KEGG	hsa:340075.
UCSC	uc003lrv.2. human.
Organism-specific databases
CTD	340075.
GeneCards	GC05M149657.
HGNC	HGNC:32521. ARSI.
HPA	HPA038386.
MIM	610009. gene.
neXtProt	NX_Q5FYB1.
PharmGKB	PA143485309.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG3119.
HOGENOM	HOG000135354.
HOVERGEN	HBG004282.
InParanoid	Q5FYB1.
KO	K12375.
OMA	YHGSDIE.
OrthoDB	EOG4DFPN6.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_17015. Metabolism of proteins.
Gene expression databases
ArrayExpress	Q5FYB1.
Bgee	Q5FYB1.
CleanEx	HS_ARSI.
Genevestigator	Q5FYB1.
GermOnline	ENSG00000183876. Homo sapiens.
Family and domain databases
Gene3D	3.40.720.10. 1 hit.
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR017850. Alkaline_phosphatase_core. IPR000917. Sulfatase. IPR024607. Sulfatase_CS. [Graphical view]
Pfam	PF00884. Sulfatase. 1 hit. [Graphical view]
SUPFAM	SSF53649. Alkaline_phosphatase_core. 1 hit.
PROSITE	PS00523. SULFATASE_1. 1 hit. PS00149. SULFATASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	340075.
NextBio	97681.
SOURCE	Search...

Entry information

Entry name

ARSI_HUMAN

Accession

Primary (citable) accession number: Q5FYB1
Secondary accession number(s): A1L3B0, B3KV22, B7XD03

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	March 1, 2005
Last modified:	May 29, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.