Reviewed,
UniProtKB/Swiss-Prot Q5FYB1 (ARSI_HUMAN)
Last modified
November 24, 2009.
Version 45.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Arylsulfatase I Short name=ASI EC=3.1.6.- | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Complete proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 569 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Displays arylsulfatase activity at neutral pH, when co-expressed with SUMF1; arylsulfatase activity is measured in the secretion medium of retinal cell line, but no activity is recorded when measured in cell extracts. |
| Cofactor | Binds 1 calcium ion per subunit By similarity. |
| Subcellular location | Secreted. Endoplasmic reticulum. Note: Localized in the intracellular granular structures. |
| Tissue specificity | Expressed in placenta, in embryonic stem cells, fetal eyes and lens. |
| Post-translational modification | The oxidation of Cys-93 residue to 3-oxoalanine (also known as C(alpha)-formylglycine) by SUMF1/Sulfatase-modifying factor 1, seems critical for catalytic activity. |
| Sequence similarities | Belongs to the sulfatase family. |
| Caution | According to Ref.2, ARSI has no arylsulfatase activity. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Secreted |
| Coding sequence diversity | Alternative splicing |
| Domain | Signal |
| Ligand | Calcium Metal-binding |
| Molecular function | Hydrolase |
| PTM | Glycoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | metabolic process Inferred from electronic annotation. Source: InterPro |
| Cellular component | extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | arylsulfatase activity Ref.1 Traceable author statement. Source: HGNC calcium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q5FYB1-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q5FYB1-2) The sequence of this isoform differs from the canonical sequence as follows: 1-143: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 23 | 23 | Potential | ||||||
| Chain | 24 – 569 | 546 | Arylsulfatase I | PRO_0000042216 | |||||
Regions | |||||||||
| Compositional bias | 527 – 533 | 7 | Poly-Glu | ||||||
Sites | |||||||||
| Active site | 149 | 1 | By similarity | ||||||
| Metal binding | 55 | 1 | Calcium By similarity | ||||||
| Metal binding | 56 | 1 | Calcium By similarity | ||||||
| Metal binding | 93 | 1 | Calcium; via 3-oxoalanine By similarity | ||||||
| Metal binding | 297 | 1 | Calcium By similarity | ||||||
| Metal binding | 298 | 1 | Calcium By similarity | ||||||
| Binding site | 147 | 1 | Substrate By similarity | ||||||
| Binding site | 239 | 1 | Substrate By similarity | ||||||
| Binding site | 315 | 1 | Substrate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 93 | 1 | 3-oxoalanine (Cys) | ||||||
| Glycosylation | 276 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 288 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 466 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 496 | 1 | N-linked (GlcNAc...) Potential | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 143 | 143 | Missing in isoform 2. | VSP_036022 | |||||
Experimental info | |||||||||
| Mutagenesis | 93 | 1 | C → S: No arylsulfatase activity in the media of retinal epithelium cell. | ||||||
| Sequence conflict | 171 | 1 | L → F in BAG53634. Ref.4 | ||||||
| Sequence conflict | 211 | 1 | L → P in BAG53634. Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sulfatases and sulfatase modifying factors: an exclusive and promiscuous relationship." Sardiello M., Annunziata I., Roma G., Ballabio A. Hum. Mol. Genet. 14:3203-3217(2005) [PubMed: 16174644] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [2] | "Molecular cloning and initial characterization of three novel human sulfatases." Obaya A.J. Gene 372:110-117(2006) [PubMed: 16500042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION. |
| [3] | "Characterization of the arylsulfatase I (ARSI) gene preferentially expressed in the human retinal pigment epithelium cell line ARPE-19." Oshikawa M., Usami R., Kato S. Mol. Vis. 15:482-494(2009) [PubMed: 19262745] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-93, OXIDATION AT CYS-93, TISSUE SPECIFICITY. |
| [4] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). |
Cross-references
Sequence databases | |
|---|---|
| AY875937 mRNA. Translation: AAW66665.1. AB448735 mRNA. Translation: BAH11166.1. AK122641 mRNA. Translation: BAG53634.1. BC129995 mRNA. Translation: AAI29996.1. BC129996 mRNA. Translation: AAI29997.1. | |
| IPI | IPI00257076. IPI00915442. |
| RefSeq | NP_001012301.1. |
| UniGene | Hs.591252 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q5FYB1. |
Proteomic databases | |
| PRIDE | Q5FYB1. |
Genome annotation databases | |
| Ensembl | ENST00000328668; ENSP00000333395; ENSG00000183876; Homo sapiens. [Genome view] |
| GeneID | 340075. |
| KEGG | hsa:340075. |
| UCSC | uc003lrv.2. human. |
Organism-specific databases | |
| CTD | 340075. |
| GeneCards | GC05M149657. |
| H-InvDB | HIX0024852. |
| HGNC | HGNC:32521. ARSI. |
| MIM | 610009. gene. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q5FYB1. |
| HOVERGEN | Q5FYB1. |
| OMA | YDNCDGP |
| OrthoDB | EOG9DFS7C |
Gene expression databases | |
| ArrayExpress | Q5FYB1. |
| Bgee | Q5FYB1. |
| CleanEx | HS_ARSI. |
| Genevestigator | Q5FYB1. |
| GermOnline | ENSG00000183876. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR017849. Alkaline_Pase-like_a/b/a. IPR017850. Alkaline_phosphatase_core. IPR000917. Sulfatase. [Graphical view] |
| Gene3D | G3DSA:3.40.720.10. Alk_phosphtse. 1 hit. |
| Pfam | PF00884. Sulfatase. 1 hit. [Graphical view] |
| PROSITE | PS00523. SULFATASE_1. 1 hit. PS00149. SULFATASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 97681. |
| SOURCE | Search... |
Entry information
| Entry name | ARSI_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q5FYB1 Secondary accession number(s): A1L3B0, B3KV22, B7XD03 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 5 Human chromosome 5: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

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