Q5FXM5 (ACBP_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-CoA-binding protein
Short name=ACBP

Cleaved into the following chain:

1. SDF-2

Gene names

Name:	acbA
ORF Names:	DDB_G0270658

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	84 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to acyl-CoA. Processed into the SDF-2 (spore differentiation factor 2) a peptide which triggers sporulation. SDF-2 appears to stimulate prestalk cells to release additional SDF-2 by acting through a signal transduction pathway that also involves dhkA, regA and PKA. Induces encapsulation of prespore cells in a dhkA-dependent manner. GABA induces the release of acbA from prespore cells and induces the exposure of tagC on the surface of prestalk cells where it can convert acbA to SDF-2. Glutamate acts as a competitive inhibitor and is also able to inhibit induction of sporulation by SDF-2. Ref.1 Ref.3 Ref.4
Subunit structure	Interacts with dhkA. Ref.4
Developmental stage	Expressed in vegetative cells but is rapidly turned over during early development before reaccumulating during late development in prespore cells but not in prestalk cells. Expression decreases in early development and reappears in late development. Highly expressed in prespore cells at the slug stage (at protein level). Ref.1
Disruption phenotype	Cells form fruiting bodies with a reduction of 90% of viable spores and without SDF-2 activity. Diazepam (Valium) can mimic SDF-2 in Dictyostelium bioassay. Ref.1
Sequence similarities	Belongs to the ACBP family. Contains 1 ACB (acyl-CoA-binding) domain.

Ontologies

Keywords
Biological process	Cytokinin signaling pathway Differentiation Sporulation Transport
Ligand	Lipid-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cytokinin mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of protein kinase activity Inferred from genetic interaction Ref.1. Source: dictyBase response to DNA damage stimulus Inferred from mutant phenotype. Source: dictyBase sporulation resulting in formation of a cellular spore Inferred from mutant phenotype Ref.1. Source: dictyBase transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from direct assay. Source: dictyBase
Molecular function	fatty-acyl-CoA binding Inferred from direct assay Ref.1. Source: dictyBase protein kinase binding Inferred from genetic interaction Ref.1. Source: dictyBase protein kinase inhibitor activity Inferred from genetic interaction Ref.1. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 84	84	Acyl-CoA-binding protein		PRO_0000328371
	Peptide	18 – 52	35	SDF-2 Potential		PRO_0000328372
Regions
	Domain	1 – 84	84	ACB

Sequences

	Sequence		Length	Mass (Da)
	Q5FXM5 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: D86989ADD83F4EFC	FASTA	84	9,431
	10 20 30 40 50 60 MTTFEEAAQK VKEFTKKPSN DELLSLYGLY KQGTDGDCNI SEPWAVQVEA KAKYNAWNAL 70 80 KGTSKEDAKA KYVALYEQLA TKYA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Peptide signaling during terminal differentiation of Dictyostelium." Anjard C., Loomis W.F. Proc. Natl. Acad. Sci. U.S.A. 102:7607-7611(2005) [PubMed: 15897458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE, SYNTHESIS OF 18-52; 18-31 AND 32-52, DISRUPTION PHENOTYPE. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Signal transduction pathways leading to spore differentiation in Dictyostelium discoideum." Anjard C., Zeng C., Loomis W.F., Nellen W. Dev. Biol. 193:146-155(1998) [PubMed: 9473320] [Abstract] Cited for: FUNCTION OF SDF-2.
[4]	"SDF-2 induction of terminal differentiation in Dictyostelium discoideum is mediated by the membrane-spanning sensor kinase DhkA." Wang N., Soderbom F., Anjard C., Shaulsky G., Loomis W.F. Mol. Cell. Biol. 19:4750-4756(1999) [PubMed: 10373524] [Abstract] Cited for: FUNCTION OF SDF-2, INTERACTION WITH DHKA.
[5]	"GABA induces terminal differentiation of Dictyostelium through a GABAB receptor." Anjard C., Loomis W.F. Development 133:2253-2261(2006) [PubMed: 16672332] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY878075 Genomic DNA. Translation: AAW70088.1. AAFI02000005 Genomic DNA. Translation: EAL72679.1.
RefSeq	XP_646321.1. XM_641229.1.
3D structure databases
HSSP	HSSP built from PDB template 1ST7 based on UniProtKB P31787.
ProteinModelPortal	Q5FXM5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5FXM5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231469; DDB0231469; DDB_G0270658.
GeneID	8617276.
GenomeReviews	Gene locus acbA in contig CM000150_GR.
KEGG	ddi:DDB_G0270658.
Organism-specific databases
dictyBase	DDB_G0270658. acbA.
Phylogenomic databases
eggNOG	KOG0817.
GeneTree	EPrGT00050000003783.
HOGENOM	HBG715417.
OMA	PTNDEFL.
PhylomeDB	Q5FXM5.
ProtClustDB	CLSZ2729302.
Family and domain databases
InterPro	IPR022408. Acyl-CoA-binding_prot_CS. IPR000582. Acyl-CoA-binding_protein. IPR014352. FERM/acyl-CoA-bd_prot_3-hlx. [Graphical view]
Gene3D	G3DSA:1.20.80.10. ACBP. 1 hit.
Pfam	PF00887. ACBP. 1 hit. [Graphical view]
PRINTS	PR00689. ACOABINDINGP.
SUPFAM	SSF47027. ACBP. 1 hit.
PROSITE	PS00880. ACB_1. 1 hit. PS51228. ACB_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACBP_DICDI

Accession

Primary (citable) accession number: Q5FXM5
Secondary accession number(s): Q55D10

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	March 1, 2005
Last modified:	September 21, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)