Reviewed, UniProtKB/Swiss-Prot Q5FXM5 (ACBP_DICDI)

Names and origin

Protein names

Recommended name:
    Acyl-CoA-binding protein
      Short name=ACBP
Cleaved into the following chain:
    1- Recommended name:
            SDF-2

Gene names

Name:	acbA
ORF Names:	DDB_G0270658

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	84 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to acyl-CoA. Processed into the SDF-2 (spore differentiation factor 2), a cytokinin-like peptide which triggers sporulation. Ref.1
Developmental stage	Expressed in vegetative cells. Expression decreases in early development and reappears in late development. Highly expressed in prespore cells at the slug stag (at protein level). Ref.1
Disruption phenotype	Cells form fruiting bodies with a reduction of 90% of viable spores and without SDF-2 activity. Diazepam (Valium) can mimic SDF-2 in Dictyostelium bioassay. Ref.1
Sequence similarities	Belongs to the ACBP family. Contains 1 ACB (acyl-CoA-binding) domain.

Ontologies

Keywords
Biological process	Sporulation Transport
Ligand	Lipid-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of protein kinase activity Ref.1 Inferred from genetic interaction. Source: dictyBase response to DNA damage stimulus Inferred from mutant phenotype. Source: dictyBase sporulation resulting in formation of a cellular spore Ref.1 Inferred from mutant phenotype. Source: dictyBase transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Ref.1 Inferred from mutant phenotype. Source: dictyBase
Molecular function	acyl-CoA binding Ref.1 Inferred from direct assay. Source: dictyBase protein kinase binding Ref.1 Inferred from genetic interaction. Source: dictyBase protein kinase inhibitor activity Ref.1 Inferred from genetic interaction. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 84	84	Acyl-CoA-binding protein		PRO_0000328371
	Peptide	18 – 52	35	SDF-2 Potential		PRO_0000328372
	Peptide	18 – 31	14	SDF-2 Potential		PRO_0000328373
	Peptide	32 – 52	21	SDF-2 Potential		PRO_0000328374
Regions
	Domain	1 – 84	84	ACB

Sequences

	Sequence		Length	Mass (Da)
	Q5FXM5-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: D86989ADD83F4EFC	FASTA	84	9,431
	10 20 30 40 50 60 MTTFEEAAQK VKEFTKKPSN DELLSLYGLY KQGTDGDCNI SEPWAVQVEA KAKYNAWNAL 70 80 KGTSKEDAKA KYVALYEQLA TKYA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Peptide signaling during terminal differentiation of Dictyostelium." Anjard C., Loomis W.F. Proc. Natl. Acad. Sci. U.S.A. 102:7607-7611(2005) [PubMed: 15897458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY878075 Genomic DNA. Translation: AAW70088.1. AAFI02000005 Genomic DNA. Translation: EAL72679.1.
RefSeq	XP_646321.1.
3D structure databases
HSSP	HSSP built from PDB template 1ST7 based on UniProtKB P31787.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5FXM5.
Genome annotation databases
GeneID	3397750.
GenomeReviews	Gene locus acbA in contig CM000150_GR.
KEGG	ddi:DDB_0231469.
Organism-specific databases
dictyBase	DDB_G0270658. acbA.
Phylogenomic databases
HOGENOM	HBG715417.
OMA	ERPDNAT.
Family and domain databases
InterPro	IPR000582. Acyl-CoA-binding_protein. IPR014352. FERM/acyl-CoA_bd_prot_3-hlx. [Graphical view]
Gene3D	G3DSA:1.20.80.10. ACBP. 1 hit.
PANTHER	PTHR23310. ACBP. 1 hit.
Pfam	PF00887. ACBP. 1 hit. [Graphical view]
PRINTS	PR00689. ACOABINDINGP.
PROSITE	PS00880. ACB_1. 1 hit. PS51228. ACB_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACBP_DICDI

Accession

Primary (citable) accession number: Q5FXM5
Secondary accession number(s): Q55D10

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	March 1, 2005
Last modified:	December 15, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)