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Q5FX52

- PLCZ1_RAT

UniProt

Q5FX52 - PLCZ1_RAT

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Protein
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
Gene
Plcz1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.By similarity1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

Cofactori

Calcium By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771 By similarityBy similarity
Active sitei222 – 2221 By similarityBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. calcium ion transport Source: Ensembl
  2. egg activation Source: Ensembl
  3. intracellular signal transduction Source: InterPro
  4. lipid catabolic process Source: UniProtKB-KW
  5. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Transducer

Keywords - Biological processi

Fertilization, Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_198661. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-zeta-1
Phospholipase C-zeta-1
Short name:
PLC-zeta-1
Gene namesi
Name:Plcz1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi1359567. Plcz1.

Subcellular locationi

Nucleus By similarity. Cytoplasmperinuclear region By similarity
Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis By similarity.By similarity

GO - Cellular componenti

  1. cytosol Source: InterPro
  2. nucleus Source: UniProtKB-SubCell
  3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6456451-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
PRO_0000347248Add
BLAST

Proteomic databases

PRIDEiQ5FX52.

PTM databases

PhosphoSiteiQ5FX52.

Expressioni

Gene expression databases

GenevestigatoriQ5FX52.

Interactioni

Subunit structurei

Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro By similarity.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011376.

Structurei

3D structure databases

ProteinModelPortaliQ5FX52.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 7736EF-hand
Add
BLAST
Domaini162 – 306145PI-PLC X-box
Add
BLAST
Domaini385 – 501117PI-PLC Y-box
Add
BLAST
Domaini506 – 608103C2
Add
BLAST

Domaini

The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity By similarity.By similarity
The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization By similarity.By similarity

Sequence similaritiesi

Contains 1 C2 domain.
Contains 1 EF-hand domain.

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00730000110266.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiQ5FX52.
KOiK05861.
OMAiMPEKDDF.
OrthoDBiEOG7V49XT.
PhylomeDBiQ5FX52.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FX52-1 [UniParc]FASTAAdd to Basket

« Hide

MESHYELAEA RWFMSKIQDY FRGGKISAGI THKLLEKLDF PCHFAHVKRI    50
FKENDRHNQG RITTEDFRTI YRCIVHREEI VEIFNTYTEN RKILPEDSLI 100
EFLTQEQYEM EMDESSSVEI IQKYEPIAEV KNERQMSIEG FARYMFSSEC 150
LLFKETCNTV YQDMNKPLND YYISSSHNTY LISDQILGPS DIWGYISALV 200
KGCRCLEIDC WDGAQNEPIV YHGYTLTSKL LFKTVIQAIN KYAFVTSDYP 250
VVLSLENHCS PGQQEVMTDI LQSTFGDFLL SDILDEFPDS LPSPEALKFK 300
ILVKNKKVGT LSETRERLGT DKRGIALDLE EEIYENEDED SGKEPETWDD 350
FLSRVKEEQE ADPSTLSGIA DAKKKIRKLR VALALSDLVI YTKAEKFRNF 400
QYSRVYQQFN ETTSMGESRA RKLSKLRAHE FIFHTAAFIT RVYPKFTRAD 450
SSNFNPQEFW NVGCQMVALN FQTPGLPMDL QNGKFLDNGG SGYVLKPDFL 500
RDTTLGFNPN EPEGDGHPVT LTIRLISGIQ LPVNVPSNTS DIIVIIEVYG 550
VPNDHMKQQS RAVKNNAFSP RWNETFTFLI QVPELALIRF VVETQGFLSG 600
NELLGQYTLP VLCMNKGYRR VPLFSKSGAN LEPSSLFIYV WYYRE 645
Length:645
Mass (Da):74,315
Last modified:March 1, 2005 - v1
Checksum:i73767CFE3AF62B8D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY885259 mRNA. Translation: AAW66659.1.
RefSeqiNP_001012234.1. NM_001012234.1.
XP_006237659.1. XM_006237597.1.
XP_006237660.1. XM_006237598.1.
UniGeneiRn.123184.

Genome annotation databases

EnsembliENSRNOT00000011376; ENSRNOP00000011376; ENSRNOG00000008514.
GeneIDi497197.
KEGGirno:497197.
UCSCiRGD:1359567. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY885259 mRNA. Translation: AAW66659.1 .
RefSeqi NP_001012234.1. NM_001012234.1.
XP_006237659.1. XM_006237597.1.
XP_006237660.1. XM_006237598.1.
UniGenei Rn.123184.

3D structure databases

ProteinModelPortali Q5FX52.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000011376.

PTM databases

PhosphoSitei Q5FX52.

Proteomic databases

PRIDEi Q5FX52.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000011376 ; ENSRNOP00000011376 ; ENSRNOG00000008514 .
GeneIDi 497197.
KEGGi rno:497197.
UCSCi RGD:1359567. rat.

Organism-specific databases

CTDi 89869.
RGDi 1359567. Plcz1.

Phylogenomic databases

eggNOGi NOG149692.
GeneTreei ENSGT00730000110266.
HOGENOMi HOG000006871.
HOVERGENi HBG053610.
InParanoidi Q5FX52.
KOi K05861.
OMAi MPEKDDF.
OrthoDBi EOG7V49XT.
PhylomeDBi Q5FX52.
TreeFami TF313216.

Enzyme and pathway databases

Reactomei REACT_198661. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

NextBioi 697710.
PROi Q5FX52.

Gene expression databases

Genevestigatori Q5FX52.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Saunders C.M.
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. "Difference in Ca2+ oscillation-inducing activity and nuclear translocation ability of PLCZ1, an egg-activating sperm factor candidate, between mouse, rat, human, and medaka fish."
    Ito M., Shikano T., Oda S., Horiguchi T., Tanimoto S., Awaji T., Mitani H., Miyazaki S.
    Biol. Reprod. 78:1081-1090(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPLCZ1_RAT
AccessioniPrimary (citable) accession number: Q5FX52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 1, 2005
Last modified: September 3, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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