Reviewed, UniProtKB/Swiss-Prot Q5FX52 (PLCZ1_RAT)

Names and origin

Protein names

Recommended name:
    1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1
    EC=3.1.4.11
Alternative name(s):
    Phospholipase C-zeta-1
      Short name=PLC-zeta-1

Gene names

Name:

Plcz1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. Ref.2 UniProtKB Q86YW0 UniProtKB Q8K4D7
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. UniProtKB P10688
Cofactor	Calcium By similarity. UniProtKB Q8K4D7
Subunit structure	Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro By similarity. UniProtKB Q8K4D7
Subcellular location	Nucleus By similarity. Cytoplasm › perinuclear region By similarity. Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis By similarity. UniProtKB Q8K4D7
Domain	The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity By similarity. UniProtKB Q8K4D7 The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization By similarity. UniProtKB Q8K4D7
Sequence similarities	Contains 1 C2 domain. Contains 1 EF-hand domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.

Ontologies

Keywords
Biological process	Fertilization Lipid degradation
Cellular component	Cytoplasm Nucleus
Ligand	Calcium
Molecular function	Developmental protein Hydrolase Transducer
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoinositide phospholipase C activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1		PRO_0000347248
Regions
	Domain	42 – 77	36	EF-hand
	Domain	162 – 306	145	PI-PLC X-box
	Domain	385 – 501	117	PI-PLC Y-box
	Domain	506 – 608	103	C2
Sites
	Active site	177	1	By similarity UniProtKB P10688
	Active site	222	1	By similarity UniProtKB P10688

Sequences

	Sequence		Length	Mass (Da)
	Q5FX52-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 73767CFE3AF62B8D	FASTA	645	74,315
	10 20 30 40 50 60 MESHYELAEA RWFMSKIQDY FRGGKISAGI THKLLEKLDF PCHFAHVKRI FKENDRHNQG 70 80 90 100 110 120 RITTEDFRTI YRCIVHREEI VEIFNTYTEN RKILPEDSLI EFLTQEQYEM EMDESSSVEI 130 140 150 160 170 180 IQKYEPIAEV KNERQMSIEG FARYMFSSEC LLFKETCNTV YQDMNKPLND YYISSSHNTY 190 200 210 220 230 240 LISDQILGPS DIWGYISALV KGCRCLEIDC WDGAQNEPIV YHGYTLTSKL LFKTVIQAIN 250 260 270 280 290 300 KYAFVTSDYP VVLSLENHCS PGQQEVMTDI LQSTFGDFLL SDILDEFPDS LPSPEALKFK 310 320 330 340 350 360 ILVKNKKVGT LSETRERLGT DKRGIALDLE EEIYENEDED SGKEPETWDD FLSRVKEEQE 370 380 390 400 410 420 ADPSTLSGIA DAKKKIRKLR VALALSDLVI YTKAEKFRNF QYSRVYQQFN ETTSMGESRA 430 440 450 460 470 480 RKLSKLRAHE FIFHTAAFIT RVYPKFTRAD SSNFNPQEFW NVGCQMVALN FQTPGLPMDL 490 500 510 520 530 540 QNGKFLDNGG SGYVLKPDFL RDTTLGFNPN EPEGDGHPVT LTIRLISGIQ LPVNVPSNTS 550 560 570 580 590 600 DIIVIIEVYG VPNDHMKQQS RAVKNNAFSP RWNETFTFLI QVPELALIRF VVETQGFLSG 610 620 630 640 NELLGQYTLP VLCMNKGYRR VPLFSKSGAN LEPSSLFIYV WYYRE

References

[1]	Saunders C.M. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Testis.
[2]	"Difference in Ca2+ oscillation-inducing activity and nuclear translocation ability of PLCZ1, an egg-activating sperm factor candidate, between mouse, rat, human, and medaka fish." Ito M., Shikano T., Oda S., Horiguchi T., Tanimoto S., Awaji T., Mitani H., Miyazaki S. Biol. Reprod. 78:1081-1090(2008) [PubMed: 18322275] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY885259 mRNA. Translation: AAW66659.1.
IPI	IPI00197811.
RefSeq	NP_001012234.1.
UniGene	Rn.123184
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000011376; ENSRNOP00000011376; ENSRNOG00000008514; Rattus norvegicus. [Genome view]
GeneID	497197.
KEGG	rno:497197.
NMPDR	fig|10116.3.peg.22468.
Organism-specific databases
CTD	497197.
RGD	1359567. Plcz1.
Phylogenomic databases
HOVERGEN	Q5FX52.
OMA	QHSRLYQ.
Gene expression databases
Genevestigator	Q5FX52.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR011992. EF-Hand_type. IPR018247. EF_HAND_1_Ca_BS. IPR018249. EF_HAND_2. IPR015359. Phospholipase_C_EF-hand-like. IPR001192. Phospholipase_C_Pinositol-sp_C. IPR000909. Phospholipase_C_Pinositol-sp_X. IPR001711. Phospholipase_C_Pinositol-sp_Y. IPR017946. PLC-like_Pdiesterase_TIM-brl. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit. G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 1 hit.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PRINTS	PR00390. PHPHLIPASEC.
ProDom	PD001202. PI_PLC_Y. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
PROSITE	PS50004. C2. 1 hit. PS00018. EF_HAND_1. False negative. PS50222. EF_HAND_2. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	697710.

Entry information

Entry name

PLCZ1_RAT

Accession

Primary (citable) accession number: Q5FX52

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	March 1, 2005
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)