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Q5FX52

- PLCZ1_RAT

UniProt

Q5FX52 - PLCZ1_RAT

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Gene

Plcz1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.By similarity1 PublicationCurated

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

    Cofactori

    Calcium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei177 – 1771By similarityPROSITE-ProRule annotation
    Active sitei222 – 2221By similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. calcium ion transport Source: Ensembl
    2. egg activation Source: Ensembl
    3. intracellular signal transduction Source: InterPro
    4. lipid catabolic process Source: UniProtKB-KW
    5. multicellular organismal development Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Hydrolase, Transducer

    Keywords - Biological processi

    Fertilization, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    ReactomeiREACT_198661. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-zeta-1
    Phospholipase C-zeta-1By similarity
    Short name:
    PLC-zeta-1By similarity
    Gene namesi
    Name:Plcz1Imported
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi1359567. Plcz1.

    Subcellular locationi

    Nucleus By similarity. Cytoplasmperinuclear region By similarity
    Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis By similarity.By similarity

    GO - Cellular componenti

    1. cytosol Source: InterPro
    2. nucleus Source: UniProtKB-SubCell
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6456451-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1PRO_0000347248Add
    BLAST

    Proteomic databases

    PRIDEiQ5FX52.

    PTM databases

    PhosphoSiteiQ5FX52.

    Expressioni

    Gene expression databases

    GenevestigatoriQ5FX52.

    Interactioni

    Subunit structurei

    Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000011376.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5FX52.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 7736EF-handPROSITE-ProRule annotationAdd
    BLAST
    Domaini162 – 306145PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini385 – 501117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini506 – 608103C2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity.By similarity
    The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization.By similarity

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 EF-hand domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    GeneTreeiENSGT00730000110266.
    HOGENOMiHOG000006871.
    HOVERGENiHBG053610.
    InParanoidiQ5FX52.
    KOiK05861.
    OMAiMPEKDDF.
    OrthoDBiEOG7V49XT.
    PhylomeDBiQ5FX52.
    TreeFamiTF313216.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR028395. PLC-zeta1.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF29. PTHR10336:SF29. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5FX52-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESHYELAEA RWFMSKIQDY FRGGKISAGI THKLLEKLDF PCHFAHVKRI    50
    FKENDRHNQG RITTEDFRTI YRCIVHREEI VEIFNTYTEN RKILPEDSLI 100
    EFLTQEQYEM EMDESSSVEI IQKYEPIAEV KNERQMSIEG FARYMFSSEC 150
    LLFKETCNTV YQDMNKPLND YYISSSHNTY LISDQILGPS DIWGYISALV 200
    KGCRCLEIDC WDGAQNEPIV YHGYTLTSKL LFKTVIQAIN KYAFVTSDYP 250
    VVLSLENHCS PGQQEVMTDI LQSTFGDFLL SDILDEFPDS LPSPEALKFK 300
    ILVKNKKVGT LSETRERLGT DKRGIALDLE EEIYENEDED SGKEPETWDD 350
    FLSRVKEEQE ADPSTLSGIA DAKKKIRKLR VALALSDLVI YTKAEKFRNF 400
    QYSRVYQQFN ETTSMGESRA RKLSKLRAHE FIFHTAAFIT RVYPKFTRAD 450
    SSNFNPQEFW NVGCQMVALN FQTPGLPMDL QNGKFLDNGG SGYVLKPDFL 500
    RDTTLGFNPN EPEGDGHPVT LTIRLISGIQ LPVNVPSNTS DIIVIIEVYG 550
    VPNDHMKQQS RAVKNNAFSP RWNETFTFLI QVPELALIRF VVETQGFLSG 600
    NELLGQYTLP VLCMNKGYRR VPLFSKSGAN LEPSSLFIYV WYYRE 645
    Length:645
    Mass (Da):74,315
    Last modified:March 1, 2005 - v1
    Checksum:i73767CFE3AF62B8D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY885259 mRNA. Translation: AAW66659.1.
    RefSeqiNP_001012234.1. NM_001012234.1.
    XP_006237659.1. XM_006237597.1.
    XP_006237660.1. XM_006237598.1.
    UniGeneiRn.123184.

    Genome annotation databases

    EnsembliENSRNOT00000011376; ENSRNOP00000011376; ENSRNOG00000008514.
    GeneIDi497197.
    KEGGirno:497197.
    UCSCiRGD:1359567. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY885259 mRNA. Translation: AAW66659.1 .
    RefSeqi NP_001012234.1. NM_001012234.1.
    XP_006237659.1. XM_006237597.1.
    XP_006237660.1. XM_006237598.1.
    UniGenei Rn.123184.

    3D structure databases

    ProteinModelPortali Q5FX52.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000011376.

    PTM databases

    PhosphoSitei Q5FX52.

    Proteomic databases

    PRIDEi Q5FX52.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000011376 ; ENSRNOP00000011376 ; ENSRNOG00000008514 .
    GeneIDi 497197.
    KEGGi rno:497197.
    UCSCi RGD:1359567. rat.

    Organism-specific databases

    CTDi 89869.
    RGDi 1359567. Plcz1.

    Phylogenomic databases

    eggNOGi NOG149692.
    GeneTreei ENSGT00730000110266.
    HOGENOMi HOG000006871.
    HOVERGENi HBG053610.
    InParanoidi Q5FX52.
    KOi K05861.
    OMAi MPEKDDF.
    OrthoDBi EOG7V49XT.
    PhylomeDBi Q5FX52.
    TreeFami TF313216.

    Enzyme and pathway databases

    Reactomei REACT_198661. Synthesis of IP3 and IP4 in the cytosol.

    Miscellaneous databases

    NextBioi 697710.
    PROi Q5FX52.

    Gene expression databases

    Genevestigatori Q5FX52.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR028395. PLC-zeta1.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF29. PTHR10336:SF29. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Saunders C.M.
      Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-DawleyImported.
      Tissue: TestisImported.
    2. "Difference in Ca2+ oscillation-inducing activity and nuclear translocation ability of PLCZ1, an egg-activating sperm factor candidate, between mouse, rat, human, and medaka fish."
      Ito M., Shikano T., Oda S., Horiguchi T., Tanimoto S., Awaji T., Mitani H., Miyazaki S.
      Biol. Reprod. 78:1081-1090(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPLCZ1_RAT
    AccessioniPrimary (citable) accession number: Q5FX52
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3