Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Gene

Plcz1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.By similarityCurated1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771PROSITE-ProRule annotationBy similarity
Active sitei222 – 2221PROSITE-ProRule annotationBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. calcium ion transport Source: Ensembl
  2. egg activation Source: Ensembl
  3. intracellular signal transduction Source: InterPro
  4. lipid catabolic process Source: UniProtKB-KW
  5. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Transducer

Keywords - Biological processi

Fertilization, Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_337837. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-zeta-1
Phospholipase C-zeta-1By similarity
Short name:
PLC-zeta-1By similarity
Gene namesi
Name:Plcz1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi1359567. Plcz1.

Subcellular locationi

  1. Nucleus By similarity
  2. Cytoplasmperinuclear region By similarity

  3. Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis (By similarity).By similarity

GO - Cellular componenti

  1. cytosol Source: InterPro
  2. nucleus Source: UniProtKB-SubCell
  3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6456451-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1PRO_0000347248Add
BLAST

Proteomic databases

PRIDEiQ5FX52.

PTM databases

PhosphoSiteiQ5FX52.

Expressioni

Gene expression databases

GenevestigatoriQ5FX52.

Interactioni

Subunit structurei

Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011376.

Structurei

3D structure databases

ProteinModelPortaliQ5FX52.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 7736EF-handPROSITE-ProRule annotationAdd
BLAST
Domaini162 – 306145PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini385 – 501117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini506 – 608103C2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity.By similarity
The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization.By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiQ5FX52.
KOiK05861.
OMAiCIVHREE.
OrthoDBiEOG7V49XT.
PhylomeDBiQ5FX52.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FX52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESHYELAEA RWFMSKIQDY FRGGKISAGI THKLLEKLDF PCHFAHVKRI
60 70 80 90 100
FKENDRHNQG RITTEDFRTI YRCIVHREEI VEIFNTYTEN RKILPEDSLI
110 120 130 140 150
EFLTQEQYEM EMDESSSVEI IQKYEPIAEV KNERQMSIEG FARYMFSSEC
160 170 180 190 200
LLFKETCNTV YQDMNKPLND YYISSSHNTY LISDQILGPS DIWGYISALV
210 220 230 240 250
KGCRCLEIDC WDGAQNEPIV YHGYTLTSKL LFKTVIQAIN KYAFVTSDYP
260 270 280 290 300
VVLSLENHCS PGQQEVMTDI LQSTFGDFLL SDILDEFPDS LPSPEALKFK
310 320 330 340 350
ILVKNKKVGT LSETRERLGT DKRGIALDLE EEIYENEDED SGKEPETWDD
360 370 380 390 400
FLSRVKEEQE ADPSTLSGIA DAKKKIRKLR VALALSDLVI YTKAEKFRNF
410 420 430 440 450
QYSRVYQQFN ETTSMGESRA RKLSKLRAHE FIFHTAAFIT RVYPKFTRAD
460 470 480 490 500
SSNFNPQEFW NVGCQMVALN FQTPGLPMDL QNGKFLDNGG SGYVLKPDFL
510 520 530 540 550
RDTTLGFNPN EPEGDGHPVT LTIRLISGIQ LPVNVPSNTS DIIVIIEVYG
560 570 580 590 600
VPNDHMKQQS RAVKNNAFSP RWNETFTFLI QVPELALIRF VVETQGFLSG
610 620 630 640
NELLGQYTLP VLCMNKGYRR VPLFSKSGAN LEPSSLFIYV WYYRE
Length:645
Mass (Da):74,315
Last modified:March 1, 2005 - v1
Checksum:i73767CFE3AF62B8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY885259 mRNA. Translation: AAW66659.1.
RefSeqiNP_001012234.1. NM_001012234.1.
XP_006237659.1. XM_006237597.2.
UniGeneiRn.123184.

Genome annotation databases

EnsembliENSRNOT00000011376; ENSRNOP00000011376; ENSRNOG00000008514.
GeneIDi497197.
KEGGirno:497197.
UCSCiRGD:1359567. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY885259 mRNA. Translation: AAW66659.1.
RefSeqiNP_001012234.1. NM_001012234.1.
XP_006237659.1. XM_006237597.2.
UniGeneiRn.123184.

3D structure databases

ProteinModelPortaliQ5FX52.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011376.

PTM databases

PhosphoSiteiQ5FX52.

Proteomic databases

PRIDEiQ5FX52.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011376; ENSRNOP00000011376; ENSRNOG00000008514.
GeneIDi497197.
KEGGirno:497197.
UCSCiRGD:1359567. rat.

Organism-specific databases

CTDi89869.
RGDi1359567. Plcz1.

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiQ5FX52.
KOiK05861.
OMAiCIVHREE.
OrthoDBiEOG7V49XT.
PhylomeDBiQ5FX52.
TreeFamiTF313216.

Enzyme and pathway databases

ReactomeiREACT_337837. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

NextBioi697710.
PROiQ5FX52.

Gene expression databases

GenevestigatoriQ5FX52.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Saunders C.M.
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-DawleyImported.
    Tissue: TestisImported.
  2. "Difference in Ca2+ oscillation-inducing activity and nuclear translocation ability of PLCZ1, an egg-activating sperm factor candidate, between mouse, rat, human, and medaka fish."
    Ito M., Shikano T., Oda S., Horiguchi T., Tanimoto S., Awaji T., Mitani H., Miyazaki S.
    Biol. Reprod. 78:1081-1090(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPLCZ1_RAT
AccessioniPrimary (citable) accession number: Q5FX52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 1, 2005
Last modified: April 1, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.