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Q5FX52 (PLCZ1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase C-zeta-1
Phospholipase C-zeta-1
Short name=PLC-zeta-1
Gene names
Name:Plcz1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length645 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. Ref.2 UniProtKB Q86YW0 UniProtKB Q8K4D7

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. UniProtKB P10688

Cofactor

Calcium By similarity. UniProtKB Q8K4D7

Subunit structure

Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro By similarity. UniProtKB Q8K4D7

Subcellular location

Nucleus By similarity. Cytoplasmperinuclear region By similarity. Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis By similarity. UniProtKB Q8K4D7

Domain

The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity By similarity. UniProtKB Q8K4D7

The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization By similarity. UniProtKB Q8K4D7

Sequence similarities

Contains 1 C2 domain.

Contains 1 EF-hand domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6456451-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
PRO_0000347248

Regions

Domain42 – 7736EF-hand
Domain162 – 306145PI-PLC X-box
Domain385 – 501117PI-PLC Y-box
Domain506 – 608103C2

Sites

Active site1771 By similarity UniProtKB P10688
Active site2221 By similarity UniProtKB P10688

Sequences

Sequence LengthMass (Da)Tools
Q5FX52 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: 73767CFE3AF62B8D

FASTA64574,315
        10         20         30         40         50         60 
MESHYELAEA RWFMSKIQDY FRGGKISAGI THKLLEKLDF PCHFAHVKRI FKENDRHNQG 

        70         80         90        100        110        120 
RITTEDFRTI YRCIVHREEI VEIFNTYTEN RKILPEDSLI EFLTQEQYEM EMDESSSVEI 

       130        140        150        160        170        180 
IQKYEPIAEV KNERQMSIEG FARYMFSSEC LLFKETCNTV YQDMNKPLND YYISSSHNTY 

       190        200        210        220        230        240 
LISDQILGPS DIWGYISALV KGCRCLEIDC WDGAQNEPIV YHGYTLTSKL LFKTVIQAIN 

       250        260        270        280        290        300 
KYAFVTSDYP VVLSLENHCS PGQQEVMTDI LQSTFGDFLL SDILDEFPDS LPSPEALKFK 

       310        320        330        340        350        360 
ILVKNKKVGT LSETRERLGT DKRGIALDLE EEIYENEDED SGKEPETWDD FLSRVKEEQE 

       370        380        390        400        410        420 
ADPSTLSGIA DAKKKIRKLR VALALSDLVI YTKAEKFRNF QYSRVYQQFN ETTSMGESRA 

       430        440        450        460        470        480 
RKLSKLRAHE FIFHTAAFIT RVYPKFTRAD SSNFNPQEFW NVGCQMVALN FQTPGLPMDL 

       490        500        510        520        530        540 
QNGKFLDNGG SGYVLKPDFL RDTTLGFNPN EPEGDGHPVT LTIRLISGIQ LPVNVPSNTS 

       550        560        570        580        590        600 
DIIVIIEVYG VPNDHMKQQS RAVKNNAFSP RWNETFTFLI QVPELALIRF VVETQGFLSG 

       610        620        630        640 
NELLGQYTLP VLCMNKGYRR VPLFSKSGAN LEPSSLFIYV WYYRE 

« Hide

References

[1]Saunders C.M.
Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Testis.
[2]"Difference in Ca2+ oscillation-inducing activity and nuclear translocation ability of PLCZ1, an egg-activating sperm factor candidate, between mouse, rat, human, and medaka fish."
Ito M., Shikano T., Oda S., Horiguchi T., Tanimoto S., Awaji T., Mitani H., Miyazaki S.
Biol. Reprod. 78:1081-1090(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY885259 mRNA. Translation: AAW66659.1.
RefSeqNP_001012234.1. NM_001012234.1.
XP_006237659.1. XM_006237597.1.
XP_006237660.1. XM_006237598.1.
UniGeneRn.123184.

3D structure databases

ProteinModelPortalQ5FX52.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000011376.

PTM databases

PhosphoSiteQ5FX52.

Proteomic databases

PRIDEQ5FX52.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000011376; ENSRNOP00000011376; ENSRNOG00000008514.
GeneID497197.
KEGGrno:497197.
UCSCRGD:1359567. rat.

Organism-specific databases

CTD89869.
RGD1359567. Plcz1.

Phylogenomic databases

eggNOGNOG149692.
GeneTreeENSGT00730000110266.
HOGENOMHOG000006871.
HOVERGENHBG053610.
InParanoidQ5FX52.
KOK05861.
OMAMPEKDDF.
OrthoDBEOG7V49XT.
PhylomeDBQ5FX52.
TreeFamTF313216.

Gene expression databases

GenevestigatorQ5FX52.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
PfamPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio697710.
PROQ5FX52.

Entry information

Entry namePLCZ1_RAT
AccessionPrimary (citable) accession number: Q5FX52
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families