Q5FWM4 (PGAM5_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PGAM5, mitochondrial
EC=3.1.3.16

Alternative name(s):
Phosphoglycerate mutase family member 5

Gene names

Name:

pgam5

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Displays phosphatase activity for serine/threonine residues. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics By similarity. May be a central mediator for programmed necrosis By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Domain	The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane By similarity.
Post-translational modification	Phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity By similarity.
Sequence similarities	Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
Sequence caution	The sequence AAH41756.1 differs from that shown. Reason: Frameshift at position 253.

Ontologies

Keywords
Biological process	Necrosis
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphoprotein phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Serine/threonine-protein phosphatase PGAM5, mitochondrial		PRO_0000288786
Regions
	Transmembrane	7 – 24	18	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5FWM4 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 79FC0F89F205D48E	FASTA	275	30,986
	10 20 30 40 50 60 MYLRRALIAG GSAAAAILGV VAAGKSKGGS DSEILSVAPP ATGQWDRNWD RREPISMVNL 70 80 90 100 110 120 SKINAETGEE ELQLHLNKHK PKATRHIFLI RHSQYKLDGK TDFDRVLTPL GREQADLTGQ 130 140 150 160 170 180 RLASLGHKYN HIVYSTMTRA KETTEIISKY LPDVNKSSSD LLREGAPIRP EPQVCHWKPD 190 200 210 220 230 240 FVYYEDGPRI EAAFRHFIHR ADPKQEEDSY EILICHANVI RYVVCRALQF PPEAWLRISL 250 260 270 NNGSITYLVI RPNGNVSIRM LGDSGFMPAE KISRT

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Egg and Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC041756 mRNA. Translation: AAH41756.1. Frameshift. BC089282 mRNA. Translation: AAH89282.1.
RefSeq	NP_001082456.1. NM_001088987.1.
UniGene	Xl.15183.
3D structure databases
ProteinModelPortal	Q5FWM4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	398484.
KEGG	xla:398484.
Organism-specific databases
CTD	192111.
Xenbase	XB-GENE-6256307. pgam5.
Phylogenomic databases
HOVERGEN	HBG105576.
KO	K15637.
Family and domain databases
InterPro	IPR013078. His_Pase_superF_clade-1. [Graphical view]
Pfam	PF00300. His_Phos_1. 1 hit. [Graphical view]
SMART	SM00855. PGAM. 1 hit. [Graphical view]
PROSITE	PS00175. PG_MUTASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGAM5_XENLA

Accession

Primary (citable) accession number: Q5FWM4
Secondary accession number(s): Q8AVL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 1, 2005
Last modified:	February 6, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program