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Protein

Serine/threonine-protein phosphatase PGAM5, mitochondrial

Gene

pgam5

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Displays phosphatase activity for serine/threonine residues. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics (By similarity). May be a central mediator for programmed necrosis (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

  1. phosphoprotein phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. programmed cell death Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Necrosis

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PGAM5, mitochondrial (EC:3.1.3.16)
Alternative name(s):
Phosphoglycerate mutase family member 5
Gene namesi
Name:pgam5
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6256307. pgam5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2418HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial outer membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Serine/threonine-protein phosphatase PGAM5, mitochondrialPRO_0000288786Add
BLAST

Post-translational modificationi

Phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5FWM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG105576.
KOiK15637.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5FWM4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYLRRALIAG GSAAAAILGV VAAGKSKGGS DSEILSVAPP ATGQWDRNWD
60 70 80 90 100
RREPISMVNL SKINAETGEE ELQLHLNKHK PKATRHIFLI RHSQYKLDGK
110 120 130 140 150
TDFDRVLTPL GREQADLTGQ RLASLGHKYN HIVYSTMTRA KETTEIISKY
160 170 180 190 200
LPDVNKSSSD LLREGAPIRP EPQVCHWKPD FVYYEDGPRI EAAFRHFIHR
210 220 230 240 250
ADPKQEEDSY EILICHANVI RYVVCRALQF PPEAWLRISL NNGSITYLVI
260 270
RPNGNVSIRM LGDSGFMPAE KISRT
Length:275
Mass (Da):30,986
Last modified:March 1, 2005 - v1
Checksum:i79FC0F89F205D48E
GO

Sequence cautioni

The sequence AAH41756.1 differs from that shown. Reason: Frameshift at position 253. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC041756 mRNA. Translation: AAH41756.1. Frameshift.
BC089282 mRNA. Translation: AAH89282.1.
RefSeqiNP_001082456.1. NM_001088987.1.
UniGeneiXl.15183.

Genome annotation databases

GeneIDi398484.
KEGGixla:398484.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC041756 mRNA. Translation: AAH41756.1. Frameshift.
BC089282 mRNA. Translation: AAH89282.1.
RefSeqiNP_001082456.1. NM_001088987.1.
UniGeneiXl.15183.

3D structure databases

ProteinModelPortaliQ5FWM4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi398484.
KEGGixla:398484.

Organism-specific databases

CTDi192111.
XenbaseiXB-GENE-6256307. pgam5.

Phylogenomic databases

HOVERGENiHBG105576.
KOiK15637.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Egg and Embryo.

Entry informationi

Entry nameiPGAM5_XENLA
AccessioniPrimary (citable) accession number: Q5FWM4
Secondary accession number(s): Q8AVL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2005
Last modified: January 7, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.