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Protein

Cell surface hyaluronidase

Gene

Tmem2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface hyaluronidase that mediates the initial cleavage of extracellular high-molecular-weight hyaluronan into intermediate-size hyaluronan of approximately 5 kDa fragments (By similarity). Acts as a regulator of angiogenesis and heart morphogenesis by mediating degradation of extracellular hyaluronan, thereby regulating VEGF signaling (By similarity). Is very specific to hyaluronan; not able to cleave chondroitin sulfate or dermatan sulfate (By similarity).By similarity

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.By similarity

Cofactori

Ca2+By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Glycosidase, Hydrolase
Biological processAngiogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Cell surface hyaluronidase1 Publication (EC:3.2.1.35By similarity)
Alternative name(s):
Transmembrane protein 21 Publication
Gene namesi
Name:Tmem21 PublicationImported
Synonyms:Kiaa14121 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1890373. Tmem2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 82CytoplasmicCuratedAdd BLAST82
Transmembranei83 – 103Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini104 – 1383ExtracellularCuratedAdd BLAST1280

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002899731 – 1383Cell surface hyaluronidaseAdd BLAST1383

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineBy similarity1
Modified residuei53PhosphoserineCombined sources1
Modified residuei63PhosphoserineBy similarity1
Glycosylationi282N-linked (GlcNAc...) asparagine; atypical1 Publication1
Glycosylationi292N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi914N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi1234N-linked (GlcNAc...) asparagine1 Publication1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ5FWI3.
PaxDbiQ5FWI3.
PeptideAtlasiQ5FWI3.
PRIDEiQ5FWI3.

PTM databases

iPTMnetiQ5FWI3.
PhosphoSitePlusiQ5FWI3.
SwissPalmiQ5FWI3.

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Developmental stagei

expressed ubiquitously at early stages of development. Expressed in the endocardial cells lining the ventricles and atria at 9.5 dpc.1 Publication

Gene expression databases

BgeeiENSMUSG00000024754.
CleanExiMM_TMEM2.
GenevisibleiQ5FWI3. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ5FWI3. 1 interactor.
MINTiMINT-4119989.
STRINGi10090.ENSMUSP00000025663.

Structurei

3D structure databases

ProteinModelPortaliQ5FWI3.
SMRiQ5FWI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini121 – 245G8PROSITE-ProRule annotationAdd BLAST125
Repeati669 – 691PbH1 1Add BLAST23
Repeati711 – 733PbH1 2Add BLAST23
Repeati791 – 812PbH1 3Add BLAST22

Sequence similaritiesi

Belongs to the TMEM2 family.Curated

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFCM. Eukaryota.
ENOG410XQ01. LUCA.
GeneTreeiENSGT00780000121902.
HOVERGENiHBG052198.
InParanoidiQ5FWI3.
KOiK22144.
OMAiQGGQNKY.
OrthoDBiEOG091G00JT.
PhylomeDBiQ5FWI3.
TreeFamiTF316575.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiView protein in InterPro
IPR019316. G8_domain.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
PfamiView protein in Pfam
PF10162. G8. 1 hit.
SMARTiView protein in SMART
SM01225. G8. 1 hit.
SUPFAMiSSF51126. SSF51126. 2 hits.
PROSITEiView protein in PROSITE
PS51484. G8. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5FWI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYAAGSRGHS PAFLQPQNGN GHRSPGYVPG KVVPLRPAPP PKNHASAKLT
60 70 80 90 100
SRSQDAPATF AFSPEEQRTP SESRKRKRHK NTFICFAITS FSFFVALAVI
110 120 130 140 150
LGISSKYAPD ENCPDQNPRL RNWDPGQDSA KHIVIKEGDL FRLTSDATVD
160 170 180 190 200
SIVIQDGGLL VFGDDKDGSK NITLRTRYIL IQDGGALHIG AEKCRYRSKA
210 220 230 240 250
TITLYGKSDE RESMPIFGKK FIGVEAGGTL ELHGAQRTSW TMLARTLHSS
260 270 280 290 300
GLPFGSYAFE KDFSRGLNVR VIDQDTARVL ENEKFDTHEY HNESRRLQEF
310 320 330 340 350
LRAQEPGRIV AIAVGDSAVK SLLQGTIQMI QDRLGSKLIQ GLGYRQAWAL
360 370 380 390 400
VGVIDGGSSS CNESVRNYEN HSTGGKALAQ GEFYTLDGQK FSVTAYSEWS
410 420 430 440 450
QGISLSGFRV DIADGVKLHL LDDVSTWEAG DRIVVASTDY SMYQAEELTL
460 470 480 490 500
LRCPECSRSQ VKVKEIPQYL HVGEIIDGID MRAEVGLLTR NIVIQGEMED
510 520 530 540 550
SCYAENHCQF FDYDTFGGHV MIEKNFTSVH LSYVELKHMG QQHMGRYPVH
560 570 580 590 600
FHLCGDVDSK GGYSQPASVD GLSVHHSFSR CITVHGTSGL LIKDTIGFDT
610 620 630 640 650
LGHCFFLEDG VEQRNILYHN LGLLTKPGTL LPTDRNSSMC TVMRDGVFGN
660 670 680 690 700
YVPVPTTDCM AVSTFWIAHP NNHLINNAAA GSQDAGIWYL FHKEPTGESS
710 720 730 740 750
GLQLLEKPEL TPLGIFYNNR VHSNFKAGLF VDKGVKTTNA SASDPREYLC
760 770 780 790 800
LDNSARFRPH QDADPEKPRV AAIIDRLIAF KNNDNGAWVR GGDIIVQNSA
810 820 830 840 850
FADNGKGLTF ASDGSFPSDE GSSQEVTESL FVGESRNYGF QGGQNKYMGT
860 870 880 890 900
GGIDQKPRTL PRNRTFPIRG FQIYDGPIHL TKSTFKKYVP TPDRYSSAIG
910 920 930 940 950
FLMKNSWQTT PRNNVSLVKF GPQVSLNVFF GKPGPWFEDC ELDGDKNSIF
960 970 980 990 1000
HDIDGSVTGY KDTYVGRMDN YLIRHPNCVN VTKWNAVICS GTYAQVYVQT
1010 1020 1030 1040 1050
WNTPNLSMII TRDEYPSHPM VLRGINQRAI SPQYQPVVML EKGYTIHWNG
1060 1070 1080 1090 1100
PAPRTTFLYL VNFNKDDWIR VGLCYPANTS FQVTVGFLQR QNGSLSRIED
1110 1120 1130 1140 1150
YEPARSMEEL QKKPSERKFY FDSGTGLLFL YLRAHSHRDG HSYCSSQGCE
1160 1170 1180 1190 1200
RVKIQAATDS KDISNCMAKA YPQYYKKPSA VKRMPAMLTG LCQGCGTHQM
1210 1220 1230 1240 1250
VFTSDPHKSY LPVRFQSPGK AEIQRGDPSI ISVNGTDFTF RSAGALLLIV
1260 1270 1280 1290 1300
DACSVPFRVK EKRMFLSADV SHMEEYFKAS IPPRSIVLLS TRGEIKQLNI
1310 1320 1330 1340 1350
SDSLAVLGLA KPAHLYSKGS VVFLGFSGNF APSWTKLFTS PDEQGLGVLE
1360 1370 1380
QFLPLQMEEY GCSRTGSVHR RDLDLLQQAL KVL
Length:1,383
Mass (Da):153,801
Last modified:March 1, 2005 - v1
Checksum:i7AB8F747A8FC659C
GO

Sequence cautioni

The sequence AAH19745 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165D → G in BAE29013 (PubMed:16141072).Curated1
Sequence conflicti1134A → S in AAF21349 (PubMed:10767548).Curated1
Sequence conflicti1161K → R in AAF21349 (PubMed:10767548).Curated1
Sequence conflicti1180A → G in AAF21349 (PubMed:10767548).Curated1
Sequence conflicti1245A → V in AAH19745 (PubMed:15489334).Curated1
Sequence conflicti1249I → V in AAH19745 (PubMed:15489334).Curated1
Sequence conflicti1306V → L in AAH19745 (PubMed:15489334).Curated1
Sequence conflicti1342D → N in AAH19745 (PubMed:15489334).Curated1
Sequence conflicti1382V → L in AAH76570 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC019745 mRNA. Translation: AAH19745.1. Different initiation.
BC076570 mRNA. Translation: AAH76570.1.
BC089353 mRNA. Translation: AAH89353.1.
AK149667 mRNA. Translation: BAE29013.1.
AK149803 mRNA. Translation: BAE29096.1.
AK129352 mRNA. Translation: BAC98162.1.
AF137031 mRNA. Translation: AAF21349.1.
CCDSiCCDS37934.1.
RefSeqiNP_001028931.1. NM_001033759.2.
NP_114386.3. NM_031997.4.
XP_006527549.1. XM_006527486.3.
XP_006527550.1. XM_006527487.3.
UniGeneiMm.329776.

Genome annotation databases

EnsembliENSMUST00000025663; ENSMUSP00000025663; ENSMUSG00000024754.
ENSMUST00000096194; ENSMUSP00000093908; ENSMUSG00000024754.
GeneIDi83921.
KEGGimmu:83921.
UCSCiuc008gzg.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiTMEM2_MOUSE
AccessioniPrimary (citable) accession number: Q5FWI3
Secondary accession number(s): Q3UE15
, Q3UE98, Q6DFZ0, Q6ZPR7, Q8VE53, Q9QY22
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: March 1, 2005
Last modified: November 22, 2017
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families