Reviewed, UniProtKB/Swiss-Prot Q5FWE3 (PRRT3_HUMAN)

Names and origin

Protein names

Recommended name:
    Proline-rich transmembrane protein 3

Gene names

Name:	PRRT3
ORF Names:	UNQ5823/PRO19642

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	981 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5FWE3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5FWE3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-384: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q5FWE3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     391-421: DEAEEWPGRPQSHPPAPPVQAPSTSRRGLIR → GESMAGVWGEGVLCLVTENLCWGCMGAEGGE
     422-981: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.4
	Chain	28 – 981	954	Proline-rich transmembrane protein 3		PRO_0000251978
Regions
	Topological domain	28 – 474	447	Extracellular Potential
	Transmembrane	475 – 495	21	Potential
	Topological domain	496 – 501	6	Cytoplasmic Potential
	Transmembrane	502 – 522	21	Potential
	Topological domain	523 – 542	20	Extracellular Potential
	Transmembrane	543 – 563	21	Potential
	Topological domain	564 – 570	7	Cytoplasmic Potential
	Transmembrane	571 – 591	21	Potential
	Topological domain	592 – 598	7	Extracellular Potential
	Transmembrane	599 – 619	21	Potential
	Topological domain	620 – 638	19	Cytoplasmic Potential
	Transmembrane	639 – 659	21	Potential
	Topological domain	660 – 679	20	Extracellular Potential
	Transmembrane	680 – 700	21	Potential
	Topological domain	701 – 981	281	Cytoplasmic Potential
	Compositional bias	247 – 462	216	Pro-rich
Amino acid modifications
	Modified residue	808	1	Phosphoserine By similarity
	Modified residue	813	1	Phosphoserine By similarity
	Modified residue	815	1	Phosphoserine By similarity
	Modified residue	854	1	Phosphoserine By similarity
	Modified residue	874	1	Phosphoserine By similarity
	Glycosylation	379	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 384	384	Missing in isoform 2.		VSP_020845
	Alternative sequence	391 – 421	31	DEAEE…RGLIR → GESMAGVWGEGVLCLVTENL CWGCMGAEGGE in isoform 3.		VSP_020846
	Alternative sequence	422 – 981	560	Missing in isoform 3.		VSP_020847
	Natural variant	138	1	Q → E: dbSNP rs279601.		VAR_027745
	Natural variant	213	1	S → P: dbSNP rs55847610. Ref.3		VAR_061695
	Natural variant	233	1	L → F: dbSNP rs55847233. Ref.3		VAR_061696
	Natural variant	334	1	R → G: dbSNP rs59465469. Ref.3		VAR_061697
	Natural variant	860	1	L → I: dbSNP rs2279794.		VAR_027746
Experimental info
	Sequence conflict	410	1	Q → K in AAH40508. Ref.2
	Sequence conflict	519	1	A → T in AAH40508. Ref.2
	Sequence conflict	819	1	A → T in AAH40508. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 13, 2007. Version 3. Checksum: 19632A8EA8CCEA1F	FASTA	981	102,197
	10 20 30 40 50 60 MASSPWGCVC GLLLLLLPLL GTGPALGRGF PRPLENSEIP MIPGAHPKGS VGSEPQAFDV 70 80 90 100 110 120 FPENPRADSH RNSDVRHAPA EEMPEKPVAS PLGPALYGPK AAQGAQRERL PVTDDLQMAQ 130 140 150 160 170 180 GPSSHGWTGP LDSQELLQQE AVAPHPVGHP HLTFIPTTPR RQLRVATVPP SLQHEGQEGQ 190 200 210 220 230 240 WPPRDEGLKA KTKSRVPPTS PSDHQGPPHT LVSHSGTVKR PVLEGQGGFE EHLQEAAQGP 250 260 270 280 290 300 HFTQQDPAAP DVGSVPPVEV VYSQEPGAQP DLALARSLPP AEELPVETPK RAGAEVSWEV 310 320 330 340 350 360 SSPGPPPKQA DLPDAKDSPG PQPTDPPASE APDRPSKPER AAMNGADPIS PQRVRGAVEA 370 380 390 400 410 420 PGTPKSLIPG PSDPGPAVNR TESPMGALQP DEAEEWPGRP QSHPPAPPVQ APSTSRRGLI 430 440 450 460 470 480 RVTTQRALGQ PPPPEPTASS MASAPASSPP ANATAPPLRW GPLRRVLSFS WELHVYGVGV 490 500 510 520 530 540 LFLLPALLAL AALAAAPAGP RLALVAAVLV LVASALRSAY MLTDPYGSQA RLGVRGGLVL 550 560 570 580 590 600 YNLPFPLLLT ALAALTLLGL GAGLPPPLQN PLLLGAVALV HGVGLLATDL LSTWSVLNLL 610 620 630 640 650 660 TQGLSCAWGA AVALGTLCLC RRRLLDGPRG WDASPGPRLL AVAGALGLLA SGLQLAAALW 670 680 690 700 710 720 LYPGPGRVGR FSWAWWGVHF WLRLLELTWA LALALAAVAA ARPRPPTEHA CWAKLMRLAC 730 740 750 760 770 780 PAPSGKSEVP ERPNNCYAGP SNVGAGSLDI SKSLIRNPAE SGQLATPSSG AWGSAASLGR 790 800 810 820 830 840 GPQGGPGLSR NGVGPAPSLS ELDLRPPSPI NLSRSIDAAL FREHLVRDSV FQRCGLRGLA 850 860 870 880 890 900 SPPPGGALRP RRGSHPKAEL DDAGSSLLRG RCRSLSDVRV RGPVPQHVVE APDGAAAAAS 910 920 930 940 950 960 GSSLDSFSRG SLKISWNPWR HGLSSVDSLP LDELPSTVQL LPAPTPAPDS TAARQGDGQG 970 980 EVQPRGKPGE SRSASSDTIE L
	Isoform 2. Checksum: BC0A570C7E086F1C	FASTA	597	61,832
	10 20 30 40 50 60 MGALQPDEAE EWPGRPQSHP PAPPVQAPST SRRGLIRVTT QRALGQPPPP EPTASSMASA 70 80 90 100 110 120 PASSPPANAT APPLRWGPLR RVLSFSWELH VYGVGVLFLL PALLALAALA AAPAGPRLAL 130 140 150 160 170 180 VAAVLVLVAS ALRSAYMLTD PYGSQARLGV RGGLVLYNLP FPLLLTALAA LTLLGLGAGL 190 200 210 220 230 240 PPPLQNPLLL GAVALVHGVG LLATDLLSTW SVLNLLTQGL SCAWGAAVAL GTLCLCRRRL 250 260 270 280 290 300 LDGPRGWDAS PGPRLLAVAG ALGLLASGLQ LAAALWLYPG PGRVGRFSWA WWGVHFWLRL 310 320 330 340 350 360 LELTWALALA LAAVAAARPR PPTEHACWAK LMRLACPAPS GKSEVPERPN NCYAGPSNVG 370 380 390 400 410 420 AGSLDISKSL IRNPAESGQL ATPSSGAWGS AASLGRGPQG GPGLSRNGVG PAPSLSELDL 430 440 450 460 470 480 RPPSPINLSR SIDAALFREH LVRDSVFQRC GLRGLASPPP GGALRPRRGS HPKAELDDAG 490 500 510 520 530 540 SSLLRGRCRS LSDVRVRGPV PQHVVEAPDG AAAAASGSSL DSFSRGSLKI SWNPWRHGLS 550 560 570 580 590 SVDSLPLDEL PSTVQLLPAP TPAPDSTAAR QGDGQGEVQP RGKPGESRSA SSDTIEL
	Isoform 3. Checksum: E5657378FCBAE080	FASTA	421	44,108
	10 20 30 40 50 60 MASSPWGCVC GLLLLLLPLL GTGPALGRGF PRPLENSEIP MIPGAHPKGS VGSEPQAFDV 70 80 90 100 110 120 FPENPRADSH RNSDVRHAPA EEMPEKPVAS PLGPALYGPK AAQGAQRERL PVTDDLQMAQ 130 140 150 160 170 180 GPSSHGWTGP LDSQELLQQE AVAPHPVGHP HLTFIPTTPR RQLRVATVPP SLQHEGQEGQ 190 200 210 220 230 240 WPPRDEGLKA KTKSRVPPTS PSDHQGPPHT LVSHSGTVKR PVLEGQGGFE EHLQEAAQGP 250 260 270 280 290 300 HFTQQDPAAP DVGSVPPVEV VYSQEPGAQP DLALARSLPP AEELPVETPK RAGAEVSWEV 310 320 330 340 350 360 SSPGPPPKQA DLPDAKDSPG PQPTDPPASE APDRPSKPER AAMNGADPIS PQRVRGAVEA 370 380 390 400 410 420 PGTPKSLIPG PSDPGPAVNR TESPMGALQP GESMAGVWGE GVLCLVTENL CWGCMGAEGG E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Brain and Chondrosarcoma.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-667 (ISOFORM 1), VARIANTS PRO-213; PHE-233 AND GLY-334. Tissue: Brain.
[4]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-42.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY358158 mRNA. Translation: AAQ88525.1. BC040508 mRNA. Translation: AAH40508.1. BC089447 mRNA. Translation: AAH89447.1. BC111555 mRNA. Translation: AAI11556.1. AK090993 mRNA. Translation: BAC03564.1.
IPI	IPI00552905. IPI00788096. IPI00788160.
RefSeq	NP_997234.3.
UniGene	Hs.598147
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5FWE3.
PTM databases
PhosphoSite	Q5FWE3.
Proteomic databases
PRIDE	Q5FWE3.
Genome annotation databases
Ensembl	ENST00000295984; ENSP00000295984; ENSG00000163704; Homo sapiens. [Genome view] ENST00000412055; ENSP00000392511; ENSG00000163704; Homo sapiens. [Genome view]
GeneID	285368.
KEGG	hsa:285368.
UCSC	uc003bul.2. human. uc003bum.2. human.
Organism-specific databases
CTD	285368.
GeneCards	GC03M009963.
HGNC	HGNC:26591. PRRT3.
PharmGKB	PA142671133.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11598.
HOGENOM	HBG126606.
HOVERGEN	Q5FWE3.
InParanoid	Q5FWE3.
OMA	WDASPGP.
OrthoDB	EOG95QKZB.
Gene expression databases
ArrayExpress	Q5FWE3.
Bgee	Q5FWE3.
CleanEx	HS_PRRT3.
Genevestigator	Q5FWE3.
GermOnline	ENSG00000163704. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	95478.

Entry information

Entry name

PRRT3_HUMAN

Accession

Primary (citable) accession number: Q5FWE3
Secondary accession number(s): Q49AD0, Q6UXY6, Q8NBC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	November 13, 2007
Last modified:	January 19, 2010
This is version 41 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.