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Protein

Major urinary protein 20

Gene

Mup20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Male pheromone which stimulates female sexual attraction to male urinary scent and promotes a strong learned attraction to the airborne urinary odor of an individual male. Promotes male aggressive behavior. Binds most of the male pheromone, 2-sec-butyl-4,5-dihydrothiazole, in urine.3 Publications

GO - Molecular functioni

  • insulin-activated receptor activity Source: UniProtKB
  • mating pheromone activity Source: MGI
  • pheromone binding Source: MGI
  • small molecule binding Source: InterPro
  • transporter activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Pheromone

Keywords - Ligandi

Pheromone-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Major urinary protein 20Imported
Alternative name(s):
Darcin1 Publication
Major urinary protein 24Imported
Gene namesi
Name:Mup20Imported
Synonyms:Mup24Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:3651981. Mup20.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • extracellular region Source: MGI
  • extracellular space Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei478. Mus m 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000039879120 – 181Major urinary protein 20By similarityAdd BLAST162

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi83 ↔ 176By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ5FW60.
PaxDbiQ5FW60.
PeptideAtlasiQ5FW60.
PRIDEiQ5FW60.

PTM databases

iPTMnetiQ5FW60.
PhosphoSitePlusiQ5FW60.

Expressioni

Tissue specificityi

Detected in urine of males but absent from female urine (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000078672.

Interactioni

GO - Molecular functioni

  • mating pheromone activity Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000073667.

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 34Combined sources4
Beta strandi35 – 37Combined sources3
Beta strandi40 – 46Combined sources7
Turni48 – 51Combined sources4
Beta strandi60 – 66Combined sources7
Beta strandi68 – 76Combined sources9
Beta strandi80 – 82Combined sources3
Beta strandi88 – 92Combined sources5
Beta strandi98 – 110Combined sources13
Beta strandi116 – 128Combined sources13
Beta strandi131 – 144Combined sources14
Helixi147 – 157Combined sources11
Helixi158 – 160Combined sources3
Turni164 – 166Combined sources3
Helixi170 – 172Combined sources3
Turni177 – 179Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L9CNMR-A20-181[»]
ProteinModelPortaliQ5FW60.
SMRiQ5FW60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the calycin superfamily. Lipocalin family.Sequence analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J5XW. Eukaryota.
ENOG411154J. LUCA.
GeneTreeiENSGT00530000063356.
HOGENOMiHOG000231458.
HOVERGENiHBG000215.
InParanoidiQ5FW60.
OMAiNEECSEI.
OrthoDBiEOG091G0Q7G.
PhylomeDBiQ5FW60.
TreeFamiTF338197.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR002345. Lipocalin.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
IPR002971. Maj_urinary.
[Graphical view]
PfamiPF00061. Lipocalin. 1 hit.
[Graphical view]
PRINTSiPR00179. LIPOCALIN.
PR01221. MAJORURINARY.
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiPS00213. LIPOCALIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5FW60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLVLLLCL GLTLVCVHAE EASSMERNFN VEKINGEWYT IMLATDKREK
60 70 80 90 100
IEEHGSMRVF VEYIHVLENS LALKFHIIIN EECSEIFLVA DKTEKAGEYS
110 120 130 140 150
VTYDGSNTFT ILKTDYDNYI MIHLINKKDG ETFQLMELYG REPDLSSDIK
160 170 180
EKFAQLSEEH GIVRENIIDL TNANRCLEAR E
Length:181
Mass (Da):20,930
Last modified:March 1, 2005 - v1
Checksum:iCBAF1D33E1B03074
GO

Mass spectrometryi

Molecular mass is 18894±2 Da from positions 20 - 181. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU882234 mRNA. Translation: ACF70718.1.
BX088584 Genomic DNA. No translation available.
CT990635 Genomic DNA. Translation: CAP58483.1.
CT990636 Genomic DNA. Translation: CAQ11567.1.
BC089613 mRNA. Translation: AAH89613.1.
BC092096 mRNA. Translation: AAH92096.1.
BK006677 Genomic DNA. Translation: DAA06315.1.
CCDSiCCDS18233.1.
RefSeqiNP_001012323.1. NM_001012323.1.
XP_006538108.1. XM_006538045.2.
UniGeneiMm.460005.

Genome annotation databases

EnsembliENSMUST00000074018; ENSMUSP00000073667; ENSMUSG00000078672.
GeneIDi381530.
KEGGimmu:381530.
UCSCiuc008tbr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU882234 mRNA. Translation: ACF70718.1.
BX088584 Genomic DNA. No translation available.
CT990635 Genomic DNA. Translation: CAP58483.1.
CT990636 Genomic DNA. Translation: CAQ11567.1.
BC089613 mRNA. Translation: AAH89613.1.
BC092096 mRNA. Translation: AAH92096.1.
BK006677 Genomic DNA. Translation: DAA06315.1.
CCDSiCCDS18233.1.
RefSeqiNP_001012323.1. NM_001012323.1.
XP_006538108.1. XM_006538045.2.
UniGeneiMm.460005.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L9CNMR-A20-181[»]
ProteinModelPortaliQ5FW60.
SMRiQ5FW60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000073667.

Protein family/group databases

Allergomei478. Mus m 1.

PTM databases

iPTMnetiQ5FW60.
PhosphoSitePlusiQ5FW60.

Proteomic databases

MaxQBiQ5FW60.
PaxDbiQ5FW60.
PeptideAtlasiQ5FW60.
PRIDEiQ5FW60.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074018; ENSMUSP00000073667; ENSMUSG00000078672.
GeneIDi381530.
KEGGimmu:381530.
UCSCiuc008tbr.1. mouse.

Organism-specific databases

CTDi381530.
MGIiMGI:3651981. Mup20.

Phylogenomic databases

eggNOGiENOG410J5XW. Eukaryota.
ENOG411154J. LUCA.
GeneTreeiENSGT00530000063356.
HOGENOMiHOG000231458.
HOVERGENiHBG000215.
InParanoidiQ5FW60.
OMAiNEECSEI.
OrthoDBiEOG091G0Q7G.
PhylomeDBiQ5FW60.
TreeFamiTF338197.

Miscellaneous databases

PROiQ5FW60.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000078672.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR002345. Lipocalin.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
IPR002971. Maj_urinary.
[Graphical view]
PfamiPF00061. Lipocalin. 1 hit.
[Graphical view]
PRINTSiPR00179. LIPOCALIN.
PR01221. MAJORURINARY.
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiPS00213. LIPOCALIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMUP20_MOUSE
AccessioniPrimary (citable) accession number: Q5FW60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 1, 2005
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.