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Protein

Major urinary protein 20

Gene

Mup20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Male pheromone which stimulates female sexual attraction to male urinary scent and promotes a strong learned attraction to the airborne urinary odor of an individual male. Promotes male aggressive behavior. Binds most of the male pheromone, 2-sec-butyl-4,5-dihydrothiazole, in urine.3 Publications

GO - Molecular functioni

  • insulin-activated receptor activity Source: UniProtKB
  • mating pheromone activity Source: MGI
  • pheromone binding Source: MGI
  • small molecule binding Source: InterPro
  • transporter activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Pheromone

Keywords - Ligandi

Pheromone-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Major urinary protein 20Imported
Alternative name(s):
Darcin1 Publication
Major urinary protein 24Imported
Gene namesi
Name:Mup20Imported
Synonyms:Mup24Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:3651981. Mup20.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • extracellular region Source: MGI
  • extracellular space Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei478. Mus m 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919By similarityAdd
BLAST
Chaini20 – 181162Major urinary protein 20By similarityPRO_0000398791Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi83 ↔ 176By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ5FW60.
PaxDbiQ5FW60.
PRIDEiQ5FW60.

PTM databases

iPTMnetiQ5FW60.
PhosphoSiteiQ5FW60.

Expressioni

Tissue specificityi

Detected in urine of males but absent from female urine (at protein level).2 Publications

Gene expression databases

BgeeiQ5FW60.

Interactioni

GO - Molecular functioni

  • mating pheromone activity Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000073667.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi31 – 344Combined sources
Beta strandi35 – 373Combined sources
Beta strandi40 – 467Combined sources
Turni48 – 514Combined sources
Beta strandi60 – 667Combined sources
Beta strandi68 – 769Combined sources
Beta strandi80 – 823Combined sources
Beta strandi88 – 925Combined sources
Beta strandi98 – 11013Combined sources
Beta strandi116 – 12813Combined sources
Beta strandi131 – 14414Combined sources
Helixi147 – 15711Combined sources
Helixi158 – 1603Combined sources
Turni164 – 1663Combined sources
Helixi170 – 1723Combined sources
Turni177 – 1793Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L9CNMR-A20-181[»]
ProteinModelPortaliQ5FW60.
SMRiQ5FW60. Positions 20-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the calycin superfamily. Lipocalin family.Sequence analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J5XW. Eukaryota.
ENOG411154J. LUCA.
GeneTreeiENSGT00530000063356.
HOGENOMiHOG000231458.
HOVERGENiHBG000215.
InParanoidiQ5FW60.
OMAiHILETDY.
OrthoDBiEOG79PJQS.
PhylomeDBiQ5FW60.
TreeFamiTF338197.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR002345. Lipocalin.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
IPR002971. Maj_urinary.
[Graphical view]
PfamiPF00061. Lipocalin. 1 hit.
[Graphical view]
PRINTSiPR00179. LIPOCALIN.
PR01221. MAJORURINARY.
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiPS00213. LIPOCALIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5FW60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLVLLLCL GLTLVCVHAE EASSMERNFN VEKINGEWYT IMLATDKREK
60 70 80 90 100
IEEHGSMRVF VEYIHVLENS LALKFHIIIN EECSEIFLVA DKTEKAGEYS
110 120 130 140 150
VTYDGSNTFT ILKTDYDNYI MIHLINKKDG ETFQLMELYG REPDLSSDIK
160 170 180
EKFAQLSEEH GIVRENIIDL TNANRCLEAR E
Length:181
Mass (Da):20,930
Last modified:March 1, 2005 - v1
Checksum:iCBAF1D33E1B03074
GO

Mass spectrometryi

Molecular mass is 18894±2 Da from positions 20 - 181. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU882234 mRNA. Translation: ACF70718.1.
BX088584 Genomic DNA. No translation available.
CT990635 Genomic DNA. Translation: CAP58483.1.
CT990636 Genomic DNA. Translation: CAQ11567.1.
BC089613 mRNA. Translation: AAH89613.1.
BC092096 mRNA. Translation: AAH92096.1.
BK006677 Genomic DNA. Translation: DAA06315.1.
CCDSiCCDS18233.1.
RefSeqiNP_001012323.1. NM_001012323.1.
XP_006538108.1. XM_006538045.1.
UniGeneiMm.460005.

Genome annotation databases

EnsembliENSMUST00000074018; ENSMUSP00000073667; ENSMUSG00000078672.
GeneIDi381530.
KEGGimmu:381530.
UCSCiuc008tbr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU882234 mRNA. Translation: ACF70718.1.
BX088584 Genomic DNA. No translation available.
CT990635 Genomic DNA. Translation: CAP58483.1.
CT990636 Genomic DNA. Translation: CAQ11567.1.
BC089613 mRNA. Translation: AAH89613.1.
BC092096 mRNA. Translation: AAH92096.1.
BK006677 Genomic DNA. Translation: DAA06315.1.
CCDSiCCDS18233.1.
RefSeqiNP_001012323.1. NM_001012323.1.
XP_006538108.1. XM_006538045.1.
UniGeneiMm.460005.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L9CNMR-A20-181[»]
ProteinModelPortaliQ5FW60.
SMRiQ5FW60. Positions 20-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000073667.

Protein family/group databases

Allergomei478. Mus m 1.

PTM databases

iPTMnetiQ5FW60.
PhosphoSiteiQ5FW60.

Proteomic databases

MaxQBiQ5FW60.
PaxDbiQ5FW60.
PRIDEiQ5FW60.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074018; ENSMUSP00000073667; ENSMUSG00000078672.
GeneIDi381530.
KEGGimmu:381530.
UCSCiuc008tbr.1. mouse.

Organism-specific databases

CTDi381530.
MGIiMGI:3651981. Mup20.

Phylogenomic databases

eggNOGiENOG410J5XW. Eukaryota.
ENOG411154J. LUCA.
GeneTreeiENSGT00530000063356.
HOGENOMiHOG000231458.
HOVERGENiHBG000215.
InParanoidiQ5FW60.
OMAiHILETDY.
OrthoDBiEOG79PJQS.
PhylomeDBiQ5FW60.
TreeFamiTF338197.

Miscellaneous databases

NextBioi402186.
PROiQ5FW60.
SOURCEiSearch...

Gene expression databases

BgeeiQ5FW60.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR002345. Lipocalin.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
IPR002971. Maj_urinary.
[Graphical view]
PfamiPF00061. Lipocalin. 1 hit.
[Graphical view]
PRINTSiPR00179. LIPOCALIN.
PR01221. MAJORURINARY.
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiPS00213. LIPOCALIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Species specificity in major urinary proteins by parallel evolution."
    Logan D.W., Marton T.F., Stowers L.
    PLoS ONE 3:E3280-E3280(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6JImported.
    Tissue: LiverImported and Submandibular glandImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/NImported.
    Tissue: LiverImported.
  4. "Structural and functional differences in isoforms of mouse major urinary proteins: a male-specific protein that preferentially binds a male pheromone."
    Armstrong S.D., Robertson D.H., Cheetham S.A., Hurst J.L., Beynon R.J.
    Biochem. J. 391:343-350(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 59-74 AND 129-141, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
    Strain: C57BL/6J1 Publication.
    Tissue: Urine1 Publication.
  5. "Identification of protein pheromones that promote aggressive behaviour."
    Chamero P., Marton T.F., Logan D.W., Flanagan K., Cruz J.R., Saghatelian A., Cravatt B.F., Stowers L.
    Nature 450:899-902(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Tissue: Urine1 Publication.
  6. "Darcin: a male pheromone that stimulates female memory and sexual attraction to an individual male's odour."
    Roberts S.A., Simpson D.M., Armstrong S.D., Davidson A.J., Robertson D.H., McLean L., Beynon R.J., Hurst J.L.
    BMC Biol. 8:75-75(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J1 Publication.
    Tissue: Urine1 Publication.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiMUP20_MOUSE
AccessioniPrimary (citable) accession number: Q5FW60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 1, 2005
Last modified: January 20, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.