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Protein

Diacylglycerol O-acyltransferase 2

Gene

Dgat2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides. Functions also as an acyl-CoA retinol acyltransferase (ARAT) (By similarity).By similarity

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Acyl-CoA + retinol = CoA + retinyl ester.

Enzyme regulationi

Inhibited by niacin.By similarity

Pathwayi: triacylglycerol biosynthesis

This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.

GO - Molecular functioni

GO - Biological processi

  • cellular response to oleic acid Source: Ensembl
  • cellular triglyceride homeostasis Source: Ensembl
  • cholesterol homeostasis Source: Ensembl
  • diacylglycerol metabolic process Source: RGD
  • fat pad development Source: Ensembl
  • fatty acid homeostasis Source: Ensembl
  • glycerol metabolic process Source: UniProtKB-KW
  • lipid storage Source: Ensembl
  • long-chain fatty-acyl-CoA metabolic process Source: Ensembl
  • low-density lipoprotein particle clearance Source: Ensembl
  • negative regulation of fatty acid oxidation Source: RGD
  • positive regulation of gluconeogenesis Source: RGD
  • positive regulation of triglyceride biosynthetic process Source: RGD
  • regulation of cholesterol metabolic process Source: RGD
  • regulation of lipoprotein metabolic process Source: RGD
  • triglyceride biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-RNO-1482883. Acyl chain remodeling of DAG and TAG.
R-RNO-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00282.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol O-acyltransferase 2 (EC:2.3.1.20)
Alternative name(s):
Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76)
Short name:
ARAT
Short name:
Retinol O-fatty-acyltransferase
Diglyceride acyltransferase 2
Gene namesi
Name:Dgat2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620329. Dgat2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6969CytoplasmicSequence analysisAdd
BLAST
Transmembranei70 – 8819HelicalSequence analysisAdd
BLAST
Topological domaini89 – 924LumenalSequence analysis
Transmembranei93 – 11220HelicalSequence analysisAdd
BLAST
Topological domaini113 – 388276CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Diacylglycerol O-acyltransferase 2PRO_0000249047Add
BLAST

Proteomic databases

PaxDbiQ5FVP8.
PRIDEiQ5FVP8.

PTM databases

PhosphoSiteiQ5FVP8.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016573.
ExpressionAtlasiQ5FVP8. baseline and differential.
GenevisibleiQ5FVP8. RN.

Interactioni

Subunit structurei

Forms multimeric complexes consisting of several DGAT2 subunits.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022557.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0831. Eukaryota.
ENOG410XTG3. LUCA.
GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
HOVERGENiHBG065791.
InParanoidiQ5FVP8.
KOiK11160.
OMAiAILMYTF.
OrthoDBiEOG091G0BGQ.
PhylomeDBiQ5FVP8.
TreeFamiTF314707.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FVP8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLIAAYSG VLRGERRAEA ARSENKNKGS ALSREGSGRW GTGSSILSAL
60 70 80 90 100
QDIFSVTWLN RSKVEKHLQV ISVLQWVLSF LVLGVACSVI LMYTFCTDCW
110 120 130 140 150
LIAALYFTWL AFDWNTPKKG GRRSQWVRNW AVWRYFRDYF PIQLVKTHNL
160 170 180 190 200
LTTRNYIFGY HPHGIMGLGA FCNFSTEATE VSKKFPGIRP YLATLAGNFR
210 220 230 240 250
MPVLREYLMS GGICPVNRDT IDYLLSKNGS GNAIVIVVGG AAESLSSMPG
260 270 280 290 300
KNAVTLRNRK GFVKLALRHG ADLVPTYSFG ENEVYKQVIF EEGSWGRWVQ
310 320 330 340 350
KKFQKYIGFA PCIFHGRGLF SSDTWGLVPY SKPITTVVGE PITVPKLEHP
360 370 380
TQKDIDLYHT MYMEALVKLF DNHKTKFGLP ETEVLEVN
Length:388
Mass (Da):43,795
Last modified:March 1, 2005 - v1
Checksum:i04DCBB134206F78D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC089846 mRNA. Translation: AAH89846.1.
AJ487787 mRNA. Translation: CAD32178.1.
RefSeqiNP_001012345.1. NM_001012345.1.
UniGeneiRn.9523.

Genome annotation databases

EnsembliENSRNOT00000022557; ENSRNOP00000022557; ENSRNOG00000016573.
GeneIDi252900.
KEGGirno:252900.
UCSCiRGD:620329. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC089846 mRNA. Translation: AAH89846.1.
AJ487787 mRNA. Translation: CAD32178.1.
RefSeqiNP_001012345.1. NM_001012345.1.
UniGeneiRn.9523.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022557.

PTM databases

PhosphoSiteiQ5FVP8.

Proteomic databases

PaxDbiQ5FVP8.
PRIDEiQ5FVP8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022557; ENSRNOP00000022557; ENSRNOG00000016573.
GeneIDi252900.
KEGGirno:252900.
UCSCiRGD:620329. rat.

Organism-specific databases

CTDi84649.
RGDi620329. Dgat2.

Phylogenomic databases

eggNOGiKOG0831. Eukaryota.
ENOG410XTG3. LUCA.
GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
HOVERGENiHBG065791.
InParanoidiQ5FVP8.
KOiK11160.
OMAiAILMYTF.
OrthoDBiEOG091G0BGQ.
PhylomeDBiQ5FVP8.
TreeFamiTF314707.

Enzyme and pathway databases

UniPathwayiUPA00282.
ReactomeiR-RNO-1482883. Acyl chain remodeling of DAG and TAG.
R-RNO-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiQ5FVP8.

Gene expression databases

BgeeiENSRNOG00000016573.
ExpressionAtlasiQ5FVP8. baseline and differential.
GenevisibleiQ5FVP8. RN.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDGAT2_RAT
AccessioniPrimary (citable) accession number: Q5FVP8
Secondary accession number(s): Q8K4Y4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 1, 2005
Last modified: September 7, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.