Reviewed, UniProtKB/Swiss-Prot Q5FVP8 (DGAT2_RAT)

Names and origin

Protein names

Recommended name:
    Diacylglycerol O-acyltransferase 2
    EC=2.3.1.20
Alternative name(s):
    Diglyceride acyltransferase 2

Gene names

Name:

Dgat2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation By similarity.
Catalytic activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Enzyme regulation	Inhibited by niacin By similarity.
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the diacylglycerol acyltransferase family.

Ontologies

Keywords
Biological process	Glycerol metabolism Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from direct assay. Source: RGD
Molecular function	diacylglycerol O-acyltransferase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Diacylglycerol O-acyltransferase 2		PRO_0000249047
Regions
	Transmembrane	70 – 90	21	Potential
	Transmembrane	92 – 112	21	Potential
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5FVP8-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 04DCBB134206F78D	FASTA	388	43,795
	10 20 30 40 50 60 MKTLIAAYSG VLRGERRAEA ARSENKNKGS ALSREGSGRW GTGSSILSAL QDIFSVTWLN 70 80 90 100 110 120 RSKVEKHLQV ISVLQWVLSF LVLGVACSVI LMYTFCTDCW LIAALYFTWL AFDWNTPKKG 130 140 150 160 170 180 GRRSQWVRNW AVWRYFRDYF PIQLVKTHNL LTTRNYIFGY HPHGIMGLGA FCNFSTEATE 190 200 210 220 230 240 VSKKFPGIRP YLATLAGNFR MPVLREYLMS GGICPVNRDT IDYLLSKNGS GNAIVIVVGG 250 260 270 280 290 300 AAESLSSMPG KNAVTLRNRK GFVKLALRHG ADLVPTYSFG ENEVYKQVIF EEGSWGRWVQ 310 320 330 340 350 360 KKFQKYIGFA PCIFHGRGLF SSDTWGLVPY SKPITTVVGE PITVPKLEHP TQKDIDLYHT 370 380 MYMEALVKLF DNHKTKFGLP ETEVLEVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[2]	"Sequencing of a partial rat DGAT2 cDNA." Waterman I.J., Zammit V.A., Price N.T. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 177-327. Strain: Wistar. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC089846 mRNA. Translation: AAH89846.1. AJ487787 mRNA. Translation: CAD32178.1.
IPI	IPI00213007.
RefSeq	NP_001012345.1.
UniGene	Rn.9523
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5FVP8.
Genome annotation databases
Ensembl	ENSRNOT00000022557; ENSRNOP00000022557; ENSRNOG00000016573; Rattus norvegicus. [Genome view]
GeneID	252900.
KEGG	rno:252900.
NMPDR	fig|10116.3.peg.2381.
UCSC	NM_001012345. rat.
Organism-specific databases
CTD	252900.
RGD	620329. Dgat2.
Phylogenomic databases
eggNOG	roNOG04766.
HOVERGEN	Q5FVP8.
InParanoid	Q5FVP8.
OMA	HGRGFFS.
OrthoDB	EOG9VX4R4.
PhylomeDB	Q5FVP8.
Enzyme and pathway databases
BRENDA	2.3.1.20. 248.
Gene expression databases
ArrayExpress	Q5FVP8.
Genevestigator	Q5FVP8.
GermOnline	ENSRNOG00000016573. Rattus norvegicus.
Family and domain databases
InterPro	IPR007130. DAGAT. [Graphical view]
PANTHER	PTHR12317. DAGAT. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	624031.

Entry information

Entry name

DGAT2_RAT

Accession

Primary (citable) accession number: Q5FVP8
Secondary accession number(s): Q8K4Y4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 1, 2005
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)