Reviewed, UniProtKB/Swiss-Prot Q5FVM4 (NONO_RAT)

Names and origin

Protein names

Recommended name:
    Non-POU domain-containing octamer-binding protein
      Short name=NonO protein

Gene names

Name:

Nono

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. Nono is involved in transcriptional regulation. The SFPQ-NONO-NR5A1/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription By similarity.
Subunit structure	Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO) and two 100 kDa (SFPQ) subunits. NONO is a component of spliceosome and U5.4/6 snRNP complexes. Interacts with PSPC1 and SNRPA/U1A. Part of complex consisting of SFPQ, NONO and MATR3. Part of a complex consisting of SFPQ, NONO and NR5A1/SF-1. Part of a complex consisting of SFPQ, NONO and TOP1. Interacts with SPI1 By similarity. Interacts with RNF43 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Contains 2 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair Transcription Transcription regulation mRNA processing mRNA splicing
Cellular component	Nucleus
Domain	Coiled coil Repeat
Ligand	DNA-binding RNA-binding
Molecular function	Activator
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Non-POU domain-containing octamer-binding protein		PRO_0000081686
Regions
	Domain	80 – 147	68	RRM 1
	Domain	154 – 235	82	RRM 2
	Region	60 – 379	320	DBHS By similarity
	Coiled coil	274 – 378	105	Potential
Amino acid modifications
	Modified residue	5	1	N6-acetyllysine By similarity
	Modified residue	11	1	N6-acetyllysine By similarity
	Modified residue	101	1	N6-acetyllysine By similarity
	Modified residue	152	1	Phosphoserine By similarity
	Modified residue	203	1	N6-acetyllysine By similarity
	Modified residue	433	1	Phosphothreonine By similarity
	Modified residue	455	1	Phosphothreonine By similarity
	Modified residue	472	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5FVM4-1 [UniParc]. Last modified July 5, 2005. Version 3. Checksum: 47521721B46E5597	FASTA	476	54,925
	10 20 30 40 50 60 MQSNKTFNLE KQNHTPRKHH QHHHQQHHQQ QQQQQQQQQQ QPPPPIPANG QQASSQNEGL 70 80 90 100 110 120 TIDLKNFRKP GEKTFTQRSR LFVGNLPPDI TEEEMRKLFE KYGKAGEVFI HKDKGFGFIR 130 140 150 160 170 180 LETRTLAEIA KVELDNMPLR GKQLRVRFAC HSASLTVRNL PQYVSNELLE EAFSVFGQVE 190 200 210 220 230 240 RAVVIVDDRG RPSGKGIVEF SGKPAARKAL DRCSEGSFLL TTFPRPVTVE PMDQLDDEEG 250 260 270 280 290 300 LPEKLVIKNQ QFHKEREQPP RFAQPGSFEY EYAMRWKALI EMEKQQQDQV DRNIKEAREK 310 320 330 340 350 360 LEMEMEAARH EHQVMLMRQD LMRRQEELRR MEELHNQEVQ KRKQLELRQE EERRRREEEM 370 380 390 400 410 420 RRQQEEMMRR QQEGFKGTFP DAREQEIRMG QMAMGGAMGI NNRGAMPPAP VPPGTPAPPG 430 440 450 460 470 PAAMMPDGTL GLTPPTTERF GQAATMEGIG AIGGTPPAFN RPAPGAEFAP NKRRRY

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC089880 mRNA. Translation: AAH89880.1.
IPI	IPI00205912.
RefSeq	NP_001012356.1.
UniGene	Rn.8381
3D structure databases
SMR	Q5FVM4. Positions 72-156, 77-211.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5FVM4.
PTM databases
PhosphoSite	Q5FVM4.
Proteomic databases
PRIDE	Q5FVM4.
Genome annotation databases
Ensembl	ENSRNOT00000004911; ENSRNOP00000004911; ENSRNOG00000003689; Rattus norvegicus. [Genome view]
GeneID	317259.
KEGG	rno:317259.
UCSC	NM_001012356. rat.
Organism-specific databases
CTD	317259.
RGD	1549738. Nono.
Phylogenomic databases
eggNOG	roNOG12392.
HOVERGEN	Q5FVM4.
InParanoid	Q5FVM4.
OMA	KQNHAPR.
OrthoDB	EOG941SXM.
Gene expression databases
ArrayExpress	Q5FVM4.
Genevestigator	Q5FVM4.
GermOnline	ENSRNOG00000003689. Rattus norvegicus.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR012975. NOPS. IPR000504. RRM_RNP1. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.
Pfam	PF08075. NOPS. 1 hit. PF00076. RRM_1. 2 hits. [Graphical view]
SMART	SM00360. RRM. 2 hits. [Graphical view]
PROSITE	PS50102. RRM. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	671515.

Entry information

Entry name

NONO_RAT

Accession

Primary (citable) accession number: Q5FVM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2005
Last modified:	January 19, 2010
This is version 48 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)