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Q5FVM4 (NONO_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Non-POU domain-containing octamer-binding protein
Short name=NonO protein
Gene names
Name:Nono
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. Nono is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription By similarity.

Subunit structure

Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO) and two 100 kDa (SFPQ) subunits. NONO is a component of spliceosome and U5.4/6 snRNP complexes. Interacts with PSPC1 and SNRPA/U1A. Part of complex consisting of SFPQ, NONO and MATR3. Part of a complex consisting of SFPQ, NONO and NR5A1. Part of a complex consisting of SFPQ, NONO and TOP1. Interacts with SPI1 By similarity. Interacts with RNF43 By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Contains 2 RRM (RNA recognition motif) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 476476Non-POU domain-containing octamer-binding protein
PRO_0000081686

Regions

Domain80 – 14768RRM 1
Domain154 – 23582RRM 2
Region60 – 379320DBHS By similarity
Coiled coil274 – 378105 Potential

Amino acid modifications

Modified residue51N6-acetyllysine By similarity
Modified residue111N6-acetyllysine By similarity
Modified residue1011N6-acetyllysine By similarity
Modified residue1521Phosphoserine By similarity
Modified residue2031N6-acetyllysine By similarity
Modified residue4331Phosphothreonine By similarity
Modified residue4551Phosphothreonine By similarity
Modified residue4721N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5FVM4 [UniParc].

Last modified July 5, 2005. Version 3.
Checksum: 47521721B46E5597

FASTA47654,925
        10         20         30         40         50         60 
MQSNKTFNLE KQNHTPRKHH QHHHQQHHQQ QQQQQQQQQQ QPPPPIPANG QQASSQNEGL 

        70         80         90        100        110        120 
TIDLKNFRKP GEKTFTQRSR LFVGNLPPDI TEEEMRKLFE KYGKAGEVFI HKDKGFGFIR 

       130        140        150        160        170        180 
LETRTLAEIA KVELDNMPLR GKQLRVRFAC HSASLTVRNL PQYVSNELLE EAFSVFGQVE 

       190        200        210        220        230        240 
RAVVIVDDRG RPSGKGIVEF SGKPAARKAL DRCSEGSFLL TTFPRPVTVE PMDQLDDEEG 

       250        260        270        280        290        300 
LPEKLVIKNQ QFHKEREQPP RFAQPGSFEY EYAMRWKALI EMEKQQQDQV DRNIKEAREK 

       310        320        330        340        350        360 
LEMEMEAARH EHQVMLMRQD LMRRQEELRR MEELHNQEVQ KRKQLELRQE EERRRREEEM 

       370        380        390        400        410        420 
RRQQEEMMRR QQEGFKGTFP DAREQEIRMG QMAMGGAMGI NNRGAMPPAP VPPGTPAPPG 

       430        440        450        460        470 
PAAMMPDGTL GLTPPTTERF GQAATMEGIG AIGGTPPAFN RPAPGAEFAP NKRRRY

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Spleen.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC089880 mRNA. Translation: AAH89880.1.
IPIIPI00205912.
RefSeqNP_001012356.1. NM_001012356.1.
UniGeneRn.8381.

3D structure databases

ProteinModelPortalQ5FVM4.
SMRQ5FVM4. Positions 72-156.
ModBaseSearch...

Protein-protein interaction databases

IntActQ5FVM4. 1 interaction.
STRINGQ5FVM4.

PTM databases

PhosphoSiteQ5FVM4.

Proteomic databases

PRIDEQ5FVM4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000004911; ENSRNOP00000004911; ENSRNOG00000003689.
GeneID317259.
KEGGrno:317259.
UCSCNM_001012356. rat.

Organism-specific databases

CTD4841.
RGD1549738. Nono.

Phylogenomic databases

eggNOGroNOG12392.
GeneTreeENSGT00390000005004.
HOVERGENHBG009801.
InParanoidQ5FVM4.
OMANQQYHKE.
OrthoDBEOG4ZCT50.

Gene expression databases

ArrayExpressQ5FVM4.
GenevestigatorQ5FVM4.
GermOnlineENSRNOG00000003689. Rattus norvegicus.

Family and domain databases

InterProIPR012975. NOPS.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
Gene3DG3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.
KOK13214.
PfamPF08075. NOPS. 1 hit.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTSM00360. RRM. 2 hits.
[Graphical view]
PROSITEPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio671515.

Entry information

Entry nameNONO_RAT
AccessionPrimary (citable) accession number: Q5FVM4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2005
Last modified: November 16, 2011
This is version 62 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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