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Protein

Sentrin-specific protease 8

Gene

Senp8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.By similarity

Catalytic activityi

Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102By similarity1
Active sitei119By similarity1
Active sitei163NucleophileBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-5689603. UCH proteinases.

Names & Taxonomyi

Protein namesi
Recommended name:
Sentrin-specific protease 8 (EC:3.4.22.68)
Alternative name(s):
Deneddylase-1
NEDD8-specific protease 1
Sentrin/SUMO-specific protease SENP8
Gene namesi
Name:Senp8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1309955. Senp8.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001017291 – 217Sentrin-specific protease 8Add BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ5FVJ8.
PRIDEiQ5FVJ8.

PTM databases

PhosphoSitePlusiQ5FVJ8.

Expressioni

Gene expression databases

BgeeiENSRNOG00000011595.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062239.

Structurei

3D structure databases

ProteinModelPortaliQ5FVJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 174ProteaseAdd BLAST164

Sequence similaritiesi

Belongs to the peptidase C48 family.Curated

Phylogenomic databases

eggNOGiKOG3246. Eukaryota.
ENOG4111G37. LUCA.
GeneTreeiENSGT00390000014038.
HOGENOMiHOG000005740.
HOVERGENiHBG054214.
InParanoidiQ5FVJ8.
KOiK08597.
OMAiFFHYDSH.
OrthoDBiEOG091G0UXY.
PhylomeDBiQ5FVJ8.
TreeFamiTF351057.

Family and domain databases

InterProiIPR003653. Peptidase_C48_C.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5FVJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPVVLSYMD SLLRQSDVSL LDPPNWLNDH IIGFAFEYFA SSQFHDCSDH
60 70 80 90 100
VCFISPEVTQ FIKCTSSPAE IAMFLEPLDL PHKRVVFLAI NDNSNQAAGG
110 120 130 140 150
THWSLLVYLQ DKNSFFHYDS HSRSNSIHAK QVAEKLKAFL GSKGDKLVFV
160 170 180 190 200
EEKAPAQQNS YDCGMYVICN TEALCQNLFR RQPESPLQLL TPTYITKKRG
210
EWKDLIARLA KKNRSSY
Length:217
Mass (Da):24,728
Last modified:March 1, 2005 - v1
Checksum:iC03009DA53071864
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC089939 mRNA. Translation: AAH89939.1.
RefSeqiNP_001012355.1. NM_001012355.1.
UniGeneiRn.145873.

Genome annotation databases

EnsembliENSRNOT00000015415; ENSRNOP00000062239; ENSRNOG00000011595.
GeneIDi315723.
KEGGirno:315723.
UCSCiRGD:1309955. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC089939 mRNA. Translation: AAH89939.1.
RefSeqiNP_001012355.1. NM_001012355.1.
UniGeneiRn.145873.

3D structure databases

ProteinModelPortaliQ5FVJ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062239.

PTM databases

PhosphoSitePlusiQ5FVJ8.

Proteomic databases

PaxDbiQ5FVJ8.
PRIDEiQ5FVJ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015415; ENSRNOP00000062239; ENSRNOG00000011595.
GeneIDi315723.
KEGGirno:315723.
UCSCiRGD:1309955. rat.

Organism-specific databases

CTDi123228.
RGDi1309955. Senp8.

Phylogenomic databases

eggNOGiKOG3246. Eukaryota.
ENOG4111G37. LUCA.
GeneTreeiENSGT00390000014038.
HOGENOMiHOG000005740.
HOVERGENiHBG054214.
InParanoidiQ5FVJ8.
KOiK08597.
OMAiFFHYDSH.
OrthoDBiEOG091G0UXY.
PhylomeDBiQ5FVJ8.
TreeFamiTF351057.

Enzyme and pathway databases

ReactomeiR-RNO-5689603. UCH proteinases.

Miscellaneous databases

PROiQ5FVJ8.

Gene expression databases

BgeeiENSRNOG00000011595.

Family and domain databases

InterProiIPR003653. Peptidase_C48_C.
[Graphical view]
PfamiPF02902. Peptidase_C48. 1 hit.
[Graphical view]
PROSITEiPS50600. ULP_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSENP8_RAT
AccessioniPrimary (citable) accession number: Q5FVJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: March 1, 2005
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.